Vigene Biosciences (Shanghai, China) constructed AAV9-shPPARα and negative shRNA (AAV9-shRNA). We purchased PPARα siRNA from Santa Cruz (SC-422360). Two weeks prior to AB surgery, randomly selected mice received an AAV9-shPPARα or AAV9-shRNA myocardial injection of 1 × 1011 vp (virion particles) per animal. Briefly, after the mice were anesthetized with 3% sodium pentobarbital (80 mg/kg, ip), the hearts of the mice were exposed and the pericardium was removed. We injected the following areas with a #29 syringe: the anterior wall, the side walls, and the apex of the left ventricle. A single needle (10 μl) was inserted at the apex of the heart, and the two needles were inserted at the anterior wall and the side wall. The total amount of each adenoviral vector injection (1 × 1011 vp) was 50 μl, and the injection interval was approximately 5 mm.
Aav9 shrna
Manufactured by Charles River Laboratories
Sourced in China
AAV9-shRNA is a laboratory product that contains adeno-associated virus serotype 9 (AAV9) carrying short hairpin RNA (shRNA). It is used as a research tool for gene silencing and knockdown experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using aav9 shrna
1
AAV9-Mediated Silencing of PPARα in Mouse Myocardium
2
AAV9-mediated gene delivery in DCM mice
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The AAV9-NEK6 and the control AAV9-NC were purchased from the Vigene Bioscience Company (Jinan, China). The AAV9-shHSP72 and the scramble RNA (AAV9-shRNA) were constructed by Vigene Bioscience (Shanghai, China). Ten weeks after the STZ injection, both the DCM mice and the sham mice were randomly assigned to receive either 60-80 μL AAV9-NEK6/ AAV9-shHSP72 (n = 12) or AAV9-NC/AAV9-shRNA (n = 12) at 5.0-6.5 × 10 13 GC/ml in sterile PBS at 37 °C by injection into the retroorbital venous plexus as described in a previous study [9] .
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