All experimental procedures were performed following an approved research protocol, which was reviewed and approved by the Institutional Animal Care and Use Committee of Sichuan Provincial People’s Hospital (2104NSF(09)). All methods were carried out in accordance with relevant guidelines and regulations. Mice were raised under a 12 h light-12 h dark cycle.
The Emc3 conditional knockout allele (Emc3fl/+) was described previously [19 (link)]. BC-specific Emc3 knockout mice were generated by mating Emc3fl/+ mice to transgenic Cre line Pcp2-Cre [20 (link)]. The F1 progeny Emc3fl/+; Pcp2-Cre mice were crossed to generate Emc3fl/fl mice (Emc3fl/fl; Pcp2-Cre, named Emc3 cKO). Emc3fl/fl, Emc3fl/+ or Emc3fl/+; Pcp2-Cre mice were used as controls. To monitor the efficiency of Cre-mediated deletion of the floxed exon, a tdTomato reporter was used (Jackson Laboratory Cat# JAX:007914).
Tissues were harvested after animals were euthanized by CO2 exposure, which was confirmed by decapitation. No animals were excluded from this study. The number of mice used in each test was listed inS2 Table .
The Emc3 conditional knockout allele (Emc3fl/+) was described previously [19 (link)]. BC-specific Emc3 knockout mice were generated by mating Emc3fl/+ mice to transgenic Cre line Pcp2-Cre [20 (link)]. The F1 progeny Emc3fl/+; Pcp2-Cre mice were crossed to generate Emc3fl/fl mice (Emc3fl/fl; Pcp2-Cre, named Emc3 cKO). Emc3fl/fl, Emc3fl/+ or Emc3fl/+; Pcp2-Cre mice were used as controls. To monitor the efficiency of Cre-mediated deletion of the floxed exon, a tdTomato reporter was used (Jackson Laboratory Cat# JAX:007914).
Tissues were harvested after animals were euthanized by CO2 exposure, which was confirmed by decapitation. No animals were excluded from this study. The number of mice used in each test was listed in