Gaspak anaerobe container system

Manufactured by BD

Sourced in United Kingdom

The GasPak anaerobe container system is a device designed to create and maintain an anaerobic environment for the cultivation of anaerobic microorganisms. It provides a controlled atmosphere for incubating microbiological samples.

Automatically generated - may contain errors

Lab products found in correlation

Dounce tissue grinder, by Merck Group (1 mentions) Lithium chloride (licl), by Merck Group (1 mentions) Vancomycin, by Merck Group (1 mentions) Sorbitol, by Merck Group (1 mentions) Sodium propionate, by Merck Group (1 mentions) M17 agar, by BD (1 mentions)

Close spelling variants of this product

BD GasPak EZ Anaerobe Container System Sachets GasPak EZ anaerobe container system GasPak system GasPaks

3 protocols using gaspak anaerobe container system

Isolation and Identification of Clostridium perfringens

Check if the same lab product or an alternative is used in the 5 most similar protocols

For isolation of C. perfringens, samples were inoculated in Difco^™ Cooked meat medium (Becton, Dickinson and Company, Sparks, MD, USA) and incubated anaerobically in 3.5 litre anaerobic jar (Oxoid Limited, Thermo Fisher Scientific Inc., UK) with GasPak^™ Anaerobe Container System (Becton, Dickinson and Company, Sparks, MD, USA) at 37°C for 24 h. Enriched samples were streaked on Sulfite Polymyxin Sulfadiazine agar plates (SPS HiVeg^™ Agar, Modified; HiMedia laboratories, Mumbai, India) and the plates were incubated anaerobically at 37°C for 24 h. After incubation suspected colonies were sub-cultured on the SPS agar plates until they were free from contaminating bacteria. The pure cultures of C. perfringens toxinotypes were lyophilized for future use in the laboratory using 0.25 M sucrose as a cryoprotectant.
Confirmation of the isolates was done by demonstration of the typical cellular morphology in Gram’s stained smear, standard biochemical tests [14 ] and detection of C. perfringens by species-specific polymerase chain reaction (PCR) using 16S rRNA gene primers [15 (link),16 ].

Nazki S., Wani S.A., Parveen R., Ahangar S.A., Kashoo Z.A., Hamid S., Dar Z.A., Dar T.A, & Dar P.A. (2017). Isolation, molecular characterization and prevalence of Clostridium perfringens in sheep and goats of Kashmir Himalayas, India. Veterinary World, 10(12), 1501-1507.

+ Open protocol

+ Expand

Isolation and Culture of Anaerobic Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols

Peritoneal fluid was obtained by peritoneal lavage (5 mL sterile PBS). Whole organs (i.e., spleen and liver) were harvested and homogenized under sterile conditions using a dounce tissue grinder (Sigma Ref: D8939-1SET). Heparanized blood was obtained by intracardial puncture. Serial dilutions were plated onto TrypticaseSoy Agar II with 5% Sheep Blood plates (Becton Dickinson Ref: 254053) and incubated (24 hr at 37°C) in air 5% CO₂ (aerobes) or in an air tight container equipped with the GasPak anaerobe container system (Becton Dickinson Ref 260678). Anaerobic conditions were confirmed in all experiments using BBL Dry Anaerobic Indicator Strips (Becton Dickinson Ref: 271051).

Weis S., Carlos A.R., Moita M.R., Singh S., Blankenhaus B., Cardoso S., Larsen R., Rebelo S., Schäuble S., Del Barrio L., Mithieux G., Rajas F., Lindig S., Bauer M, & Soares M.P. (2017). Metabolic Adaptation Establishes Disease Tolerance to Sepsis. Cell, 169(7), 1263-1275.e14.

+ Open protocol

+ Expand

Survival Assessment of Probiotic Bacteria

Check if the same lab product or an alternative is used in the 5 most similar protocols

For evaluating the survival of bacteria, grated cheese (12.5 g) was blended into 112.5 mL of peptone water (0.15% wt/vol). Serial dilutions were made and each type of bacteria was enumerated on appropriate agar as described below (Tharmaraj and Shah 2003; Dave and Shah 1996; Lapierre and others 1992) . Anaerobic conditions, where required, was maintained using anaerobic jars and GasPak anaerobe container system (Becton Dickinson and Co.). Gram staining was carried out and colony morphology was closely monitored to ensure the growth of a pure culture. Streptococcus thermophilus MS were enumerated on M17 agar (Becton Dickinson and Co.) and aerobically incubated at 37 °C for 24 h. Lactobacillus delbrueckii ssp. bulgaricus 859 were enumerated using pH-modified (pH 5.2) MRS agar plates and incubated anaerobically at 40 °C for 48 h. Lactobacillus acidophilus was enumerated on MRS agar supplemented with sorbitol (10% wt/vol; Sigma Aldrich, St. Louis, Mo., U.S.A.) and Lactobacillus casei on MRS agar supplemented with vancomycin (2 mL of 0.5 mg/mL vancomycin per liter of media; Sigma Aldrich), and both were incubated anaerobically at 37 °C for 48 h. Bifidobacterium longum was enumerated using MRS agar supplemented with sodium propionate (3% wt/vol; Sigma Aldrich) and lithium chloride (2% wt/vol; Sigma Aldrich) and the plates were incubated anaerobically at 40 °C for 36 to 48 h.

Gandhi A, & Shah N.P. (2016). Salt Reduction in a Model High-Salt Akawi Cheese: Effects on Bacterial Activity, pH, Moisture, Potential Bioactive Peptides, Amino Acids, and Growth of Human Colon Cells. Journal of food science, 81(4).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!