Zymosan a from

Manufactured by Merck Group

Sourced in United States

Zymosan A is a laboratory reagent derived from the cell wall of the yeast Saccharomyces cerevisiae. It is used as a tool for research in various fields, such as immunology and inflammation studies. Zymosan A is composed of a complex mixture of polysaccharides, proteins, and other components, which can be used to stimulate specific immune responses in experimental models.

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Lab products found in correlation

2 protocols using zymosan a from

Zymosan-Induced Inflammation Protocol

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WT mice were injected with zymosan A (from Saccharomyces cerevisiae, Sigma-Aldrich) at 100mg/kg, i.p. since the lower dose of zymosan have no significant effect on rectal temperature ⁴. In the experiments the mice received saline or RSL3 (40mg/kg) or IKE (imidazole ketone erastin, (50mg/kg) with /without DETA NONOate (120mg/kg), i.p. injection at 72 h after zymosan administration, and the peritoneal exudates were collected ⁵ at 5 h after the RSL3 and/or DETA NONOate administration.

Kapralov A.A., Yang Q., Dar H.H., Tyurina Y.Y., Anthonymuthu T.S., Kim R., St. Croix C.M., Mikulska-Ruminska K., Liu B., Shrivastava I.H., Tyurin V.A., Ting H.C., Wu Y.L., Gao Y., Shurin G.V., Artyukhova M.A., Ponomareva L.A., Timashev P.S., Domingues R.M., Stoyanovsky D.A., Greenberger J.S., Mallampalli R.K., Bahar I., Gabrilovich D.I., Bayir H, & Kagan V.E. (2020). Redox Lipid Reprogramming Commands Susceptibility of Macrophages and Microglia to Ferroptotic Death.. Nature chemical biology, 16(3), 278-290.

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Neutrophil Activation and Inflammation Assay

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Zymosan A from Saccharomyses cerevisiae, luminol (3-aminophthalhydrazide), N-formyl-methionyl-leucylphenylalanine, phorbol 12-myristate 13-acetate, Hanks Balance Salt Solution, phosphate buffer saline (PBS), ficoll, dimethyl sulfoxide, trypan blue, acetylsalicylic acid (purity 99%), ibuprofen (purity 99%) and indomethacin (purity 99%) were from Sigma (St Louis, MO, USA). Haematoxylin and xylene were from BDH, UK. Lymphoprep® was from Stemcell Technology, USA. MPO activity colorimetric assay kit was from Biovison, UK. Brain heart infusion, Muller-Hinton broth, and agar were from the Becton, Dickinson, and Company (Franklin Lakes, NJ).

Septama A.W., Jantan I., Panichayupakaranant P., Aluwi M.F.F.M., & Rahmi E.P. (2020). Immunosuppressive and antibacterial activities of dihydromorin and norartocarpetin isolated from Artocarpus heterophyllus heartwoods. Asian Pacific Journal of Tropical Biomedicine, 10(8), 361-361.

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