Mouse gla osteocalcin high sensitive eia kit

Manufactured by Takara Bio

Sourced in Japan, United States

The Mouse Gla-Osteocalcin High Sensitive EIA Kit is a laboratory equipment product designed for the quantitative determination of Gla-Osteocalcin in mouse serum or plasma samples. It utilizes an enzyme immunoassay (EIA) technique to measure the target analyte.

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15 protocols using mouse gla osteocalcin high sensitive eia kit

Measuring Metabolic Biomarkers in Mice

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Blood glucose was measured using a glucometer (Life Scan, Inc., Milpitas, CA, USA) immediately following decapitation. Serum corticosterone (ng/ml) and osteocalcin (ng/ml) were measured using a mouse Corticosterone ELISA kit obtained from Abcam (Cambridge, MA, USA) and a mouse Gla-osteocalcin High Sensitive EIA kit obtained from Clontech (Takara Bio Inc Shiga, Japan), respectively.

Keune J.A., Branscum A.J., Wong C.P., Iwaniec U.T, & Turner R.T. (2019). Effect of Leptin Deficiency on the Skeletal Response to Hindlimb Unloading in Adult Male Mice. Scientific Reports, 9, 9336.

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Serum Osteocalcin Measurement Protocol

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Serum osteocalcin was measured using mouse Gla-osteocalcin High Sensitive EIA kit from Clontech (Mountain View, CA).

Philbrick K.A., Wong C.P., Kahler-Quesada A.M., Olson D.A., Branscum A.J., Turner R.T, & Iwaniec U.T. (2018). Polyethylene particles inserted over calvarium induce cancellous bone loss in femur in female mice. Bone Reports, 9, 84-92.

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Serum Biomarkers for Skeletal Health

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Serum leptin was measured using Mouse Leptin Quantikine ELISA Kit (R&D Systems, Minneapolis, MN), serum osteocalcin was measured using Mouse Gla-Osteocalcin High Sensitive EIA Kit (Clontech, Mountain View, CA), and serum CTX-1 was measured using Mouse CTX-1 ELISA kit (Life Sciences Advanced Technologies, Petersburg, FL) according to the respective manufacturer's protocol.

Turner R.T., Philbrick K.A., Wong C.P., Gamboa A.R., Branscum A.J, & Iwaniec U.T. (2020). Effects of Propranolol on Bone, White Adipose Tissue, and Bone Marrow Adipose Tissue in Mice Housed at Room Temperature or Thermoneutral Temperature. Frontiers in Endocrinology, 11, 117.

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Measuring Serum Markers in Mice

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Serum leptin was measured using Mouse Leptin Quantikine ELISA Kit (R&D Systems, Minneapolis, MN), serum osteocalcin was measured using Mouse Gla-Osteocalcin High Sensitive EIA Kit (Clontech, Mountain View, CA), and serum Mouse C terminal telopeptides of type I collagen (CTX-1) was measured using ELISA kit (Life Sciences Advanced Technologies, St. Petersburg, FL) according to the respective manufacturer’s protocol. Intra-assay coefficient of variation (CV) for all ELISA assays were within the manufactures’ reported CVs of ≤ 5%.

Turner R.T., Nesser K.L., Philbrick K.A., Wong C.P., Olson D.A., Branscum A.J, & Iwaniec U.T. (2022). Leptin and environmental temperature as determinants of bone marrow adiposity in female mice. Frontiers in Endocrinology, 13, 959743.

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Quantifying Serum Biomarkers in Mice

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Serum leptin was measured using Mouse Leptin Quantikine ELISA Kit (R&D Systems), serum osteocalcin was measured using Mouse Gla-Osteocalcin High Sensitive EIA Kit (Clontech, Mountain View, CA, USA), and serum CTX-1 was measured using Mouse CTX-1 ELISA kit (Life Sciences Advanced Technologies, St. Petersburg, FL, USA) according to the respective manufacturer’s protocol.

Martin S.A., Philbrick K.A., Wong C.P., Olson D.A., Branscum A.J., Jump D.B., Marik C.K., DenHerder J.M., Sargent J.L., Turner R.T, & Iwaniec U.T. (2019). Thermoneutral housing attenuates premature cancellous bone loss in male C57BL/6J mice. Endocrine Connections, 8(11), 1455-1467.

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Serum Biomarkers of Bone Metabolism

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Serum leptin was measured using Mouse Leptin Quantikine ELISA Kit (R&D Systems, Minneapolis, MN), serum osteocalcin was measured using Mouse Gla-Osteocalcin High Sensitive EIA Kit (Clontech, Mountain View, CA), and serum CTx-1 was measured using Mouse C terminal telopeptides of type I collagen (CTx-1) ELISA kit (Life Sciences Advanced Technologies, St. Petersburg, FL) according to the respective manufacturer’s protocol. Intra-assay coefficient of variation (CV) for all ELISA assays were within the manufactures’ reported CVs of ≤ 5% for osteocalcin and leptin and ≤ 10% for CTx-1.

Philbrick K.A., Wong C.P., Branscum A.J., Turner R.T, & Iwaniec U.T. (2017). Leptin Stimulates Bone Formation in ob/ob Mice at Doses Having Minimal Impact on Energy Metabolism. The Journal of endocrinology, 232(3), 461-474.

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Osteocalcin and CTx Quantification

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Serum osteocalcin was measured using mouse Gla-osteocalcin High Sensitive EIA kit from Clontech (Mountain View, CA). Serum CTx was measured using mouse CTx ELISA kit from Life Sciences Advanced Technologies (Saint Petersburg, FL).

Turner R.T., Philbrick K.A., Wong C.P., Olson D.A., Branscum A.J, & Iwaniec U.T. (2014). Morbid Obesity Attenuates the Skeletal Abnormalities Associated with Leptin Deficiency in Mice. The Journal of endocrinology, 223(1), M1-M15.

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Serum Biomarkers in Mouse Studies

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Serum leptin was measured using Mouse Leptin Quantikine ELISA Kit (R&D Systems, Minneapolis, MN), serum osteocalcin was measured using Mouse Gla-Osteocalcin High Sensitive EIA Kit (Clontech, Mountain View, CA), and serum CTX-1 was measured using Mouse CTX-1 ELISA kit (Life Sciences Advanced Technologies, Petersburg, FL) according to the respective manufacturer’s protocol.

Iwaniec U.T., Philbrick K.A., Wong C.P., Gordon J.L., Kahler-Quesada A.M., Olson D.A., Branscum A.J., Sargent J.L., DeMambro V.E., Rosen C.J, & Turner R.T. (2016). Room Temperature Housing Results in Premature Cancellous Bone Loss in Growing Female Mice: Implications for the Mouse as a Preclinical Model for Age-Related Bone Loss. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA, 27(10), 3091-3101.

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Measurement of Bone and Hormonal Markers

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The serum level of total Ocn was examined using the Ocn EIA kit (BTI Biomedical Technologies, Inc., Stoughton. MA), that of carboxyleted Ocn by the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Takara Bio Inc., Shiga Japan), that of uncarboxyleted Ocn by the Mouse Glu-Osteocalcin High Sensitive EIA Kit (Takara Bio Inc.), that of P1NP by Rat/Mouse P1NP ELISA (Immunodiagnostic Systems, Boldon, UK), that of TRAP5b by the Mouse TRAP Assay (Immunodiagnostic Systems), and that of CTX1 by RatLaps (CTX-I) EIA (Immunodiagnostic Systems). Serum testosterone levels were assessed using Testosterone Rat/Mouse ELISA (Demeditec Diagnostics GmbH, Kiel, Germany).

Moriishi T., Ozasa R., Ishimoto T., Nakano T., Hasegawa T., Miyazaki T., Liu W., Fukuyama R., Wang Y., Komori H., Qin X., Amizuka N, & Komori T. (2020). Osteocalcin is necessary for the alignment of apatite crystallites, but not glucose metabolism, testosterone synthesis, or muscle mass. PLoS Genetics, 16(5), e1008586.

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Measuring Serum Osteocalcin and CTX-1

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Serum osteocalcin was measured using mouse Gla-Osteocalcin High Sensitive EIA Kit (TaKaRa Bio Inc., Mountain View, CA). Serum CTX-1 was measured using mouse CTX-1 ELISA kit (MyBiosource, San Diego, CA).

Deyhle RT J.r., Wong C.P., Martin S.A., McDougall M.Q., Olson D.A., Branscum A.J., Menn S.A., Iwaniec U.T., Hamby D.M, & Turner R.T. (2019). Maintenance of Near Normal Bone Mass and Architecture in Lethally Irradiated Female Mice following Adoptive Transfer with as few as 750 Purified Hematopoietic Stem Cells. Radiation research, 191(5), 413-427.

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