Cd123 pe

Manufactured by Thermo Fisher Scientific

Sourced in United States

CD123-PE is a fluorescent-labeled antibody product produced by Thermo Fisher Scientific. It is used for the detection and analysis of CD123-expressing cells in flow cytometry applications.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

CD123-PE-Cy7 (3 citations)

3 protocols using cd123 pe

Isolating Hematopoietic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mononuclear cells were isolated from 5 young (24–37 years old) donors and 4 aged (64–71 years old) donors with Ficoll-Paque (GE Healthcare), then enriched using CD34 MicroBeads on a Automacs Pro separator (Miltenyi, San Diego, CA). CD34+ cells were stained with lineage antibodies conjugated to PerCP-Cy™5.5: CD2 (Becton, Dickinson and Company, clone RPA-2.10), CD3 (Becton, Dickinson and Company, clone UCHT1), CD4 (Becton, Dickinson and Company, clone RPA-T4), CD7 (Becton, Dickinson and Company, clone M-T701), CD8 (Becton, Dickinson and Company, clone RPA-T8), CD10 (Becton, Dickinson and Company, clone HI10a), CD11b (Biolegend, clone ICRF44), CD14 (Becton, Dickinson and Company, clone M5E2), CD19 (eBioscience, clone HIB19), CD20 (eBioscience, clone 2H7), CD24 (Biolegend, clone ML5), CD56 (Becton, Dickinson and Company, clone B159), CD66b (Becton, Dickinson and Company, clone G10F5), Glycophorin A (Biolegend, clone HIR2) and CD90-biotin (eBioscience, clone 5E10). To isolate HSCe, lineage stained cells were stained with: Streptavidin APC-Cy7 (Becton, Dickinson and Company), CD123-PE (eBioscience, clone 6H6), CD34-APC (eBioscience, clone 4H11), CD38-PE-Cy7 (eBioscience, clone HIT2), CD45RA-FITC (Invitrogen, clone MEM-56). Cell sorting was performed on a BD FACSAria II with a 100 μm nozzle.

Adelman E.R., Huang H.T., Roisman A., Olsson A., Colaprico A., Qin T., Lindsley R.C., Bejar R., Salomonis N., Grimes H.L, & Figueroa M.E. (2019). Aging Human Hematopoietic Stem Cells Manifest Profound Epigenetic Reprogramming of Enhancers That May Predispose to Leukemia. Cancer discovery, 9(8), 1080-1101.

+ Open protocol

+ Expand

Multiparametric Analysis of Lung Leukocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

TKO-BLT-L mice were bled at 8- and 12-weeks post-surgery. Blood leukocytes were purified using RBC Lysis buffer (BioLegend, San Diego, CA, USA). Lung organoids were collected and digested using 60 mg/mL collagenase D (Roche, Basil, Switzerland) and 10 U/mL DNAse I for 1 h before passage through 70 mm filters. Leukocytes were then isolated using a 40%/70% Percoll gradient. Cells were incubated with anti-human Fc blocker (Thermo Fisher Scientific, Waltham, MA, USA) before antibody staining. Anti-mouse specific CD45, was used to exclude mouse leukocytes. Antibodies used included: CD3 V450, CD4 APC, CD8 APC-eFluor 780, CD14 PC7, CD16 FITC, CD19 PerCP-Cy5.5, CD45 V500, Live/Dead-PE-Texas Red, CD123 PE (eBioscience, San Diego, CA, USA), CD33 PE (Miltenyi, Bergisch Gladbach, Germany), CD56-AF700, CD11c-APC, HLA-DR and Lin-1 (CD3, CD14, CD16, CD19, CD20, CD56)-FITC (Biolegend). Data were acquired on a BD CytoFlex (Beckman Coulter, Brea, CA, USA) and analyzed with FlowJo Version 10.6 (BD Biosciences, Franklin Lakes, NJ, USA).

Di Y., Lew J., Goncin U., Radomska A., Rout S.S., Gray B.E., Machtaler S., Falzarano D, & Lavender K.J. (2022). SARS-CoV-2 Variant-Specific Infectivity and Immune Profiles Are Detectable in a Humanized Lung Mouse Model. Viruses, 14(10), 2272.

+ Open protocol

+ Expand

Isolation and Characterization of Plasmacytoid Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood obtained in heparin containing Vacutainer tubes (BD Bioscience) was processed within 1 hour of drawing. PBMCs were isolated by Ficoll Plaque Plus (GE Healthcare/Amersham Bioscience) density gradient centrifugation according to the manufacturer’s instructions. In several experiments, pDCs were depleted from PBMCs using a CD304 (BDCA-4/Neuropilin-1) Microbead Kit from Miltenyi Biotec (Germany) according to the manufacturer’s instructions. For assessment of the pDC frequency and the effectiveness of the depletion method, PBMCs were stained before and after the pDC depletion using the following antibodies: anti-HLA DR-Pacific Blue and CD11c-APC (BioLegend, San Diego, CA, USA), Lineage Cocktail 1 (Lin1)-fluorescein isothiocyanate (FITC, BD, Franklin Lakes, NJ, USA), and CD123-PE (eBioscience, San Diego, CA, USA).

Damjanovska S., Smith C., Sayin I., Burant C.J., Gravenstein S, & Canaday D.H. (2020). Adjuvant Effect of Type I Interferon Induced by Many but Not All Commercial Influenza Vaccines. Vaccine, 39(5), 786-789.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!