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8.0 μm pet cell culture insert

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The 8.0 μm PET cell culture insert is a laboratory equipment used for cell culture applications. It provides a permeable membrane substrate for the cultivation and study of cells in vitro.

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Lab products found in correlation

Axiovert observer a1, by Zeiss (2 mentions)

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Cell culture inserts (225 citations) Culture insert (7 citations) 8-μm cell culture inserts (4 citations) 8.0-μm cell culture inserts (3 citations)

2 protocols using 8.0 μm pet cell culture insert

1

Cell Migration Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
40,000 cells in serum-free media were added to the top chamber of an 8.0 μm PET cell culture insert (Falcon) with conditioned media (72 hr) from indicated cells in the bottom reservoir. After 12 (HeyA8) or 18 (TYK-nu) hr cells were fixed with 4% PFA in PBS, representative fluorescent images collected with a Zeiss Axiovert Observer.A1, stained with 0.1% crystal violet, washed extensively with PBS, and dried. Crystal violet was released with 10% acetic acid and the absorbance at 520 nm measured to quantify relative migration.
Eckert M.A., Coscia F., Chryplewicz A., Chang J.W., Hernandez K.M., Pan S., Tienda S.M., Nahotko D.A., Li G., Blaženović I., Lastra R.R., Curtis M., Yamada S.D., Perets R., McGregor S.M., Andrade J., Fiehn O., Moellering R.E., Mann M, & Lengyel E. (2019). Proteomics reveals NNMT as a master metabolic regulator of cancer associated fibroblasts. Nature, 569(7758), 723-728.
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2

Cell Migration Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
40,000 cells in serum-free media were added to the top chamber of an 8.0 μm PET cell culture insert (Falcon) with conditioned media (72 hr) from indicated cells in the bottom reservoir. After 12 (HeyA8) or 18 (TYK-nu) hr cells were fixed with 4% PFA in PBS, representative fluorescent images collected with a Zeiss Axiovert Observer.A1, stained with 0.1% crystal violet, washed extensively with PBS, and dried. Crystal violet was released with 10% acetic acid and the absorbance at 520 nm measured to quantify relative migration.
Eckert M.A., Coscia F., Chryplewicz A., Chang J.W., Hernandez K.M., Pan S., Tienda S.M., Nahotko D.A., Li G., Blaženović I., Lastra R.R., Curtis M., Yamada S.D., Perets R., McGregor S.M., Andrade J., Fiehn O., Moellering R.E., Mann M, & Lengyel E. (2019). Proteomics reveals NNMT as a master metabolic regulator of cancer associated fibroblasts. Nature, 569(7758), 723-728.
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