Stinggt

WT C57BL/6J (strain 000664), STING-KO, strain 025805), and STING^GT (strain 017537) mice were purchased from The Jackson Laboratory and were housed in ventilated microisolator cages in 12-hour light/dark cycles. STING-KO mice lack exons 3–5 of the transmembrane protein 173 (Tmem173) gene, and Golden Ticket (Tmem173^GT) is an I199N missense mutant allele of the STING gene, which does not produce IFN-β in response to cyclic dinucleotides or Listeria monocytogenes infection, as indicated on The Jackson Laboratory website. At 2 months of age, mice were randomly selected from each strain. Insulin-deficient diabetes was induced in these mice by i.p. injection of STZ (S0130, Sigma-Aldrich) for 5 consecutive days at a concentration of 60 mg/kg (BW) and insulin (0–0.2 units every 2–3 days; 12585014, Invitrogen) was administered as needed to prevent weight loss while still allowing chronic hyperglycemia. In addition, fasting blood glucose was measured as previously reported (3 (link)). The onset of diabetes was determined when blood glucose readings exceeded 275 mg/dL consistently on 3 different days. HbA1c was measured using the Mouse HbA1c Assay Kit (80310, Crystal Chem) and its control (80313, Crystal Chem) when the mice were euthanized to assess the severity of hyperglycemia. Clinical data from these diabetic mice are shown in Supplemental Table 4.