Data analyses were carried out in Excel 2018 and GraphPad Prism 8. The change in the TIL expansion, phenotypic subpopulations, specificity and tumor reactivity was investigated for the statistical difference using Wilcoxon matched-pairs rank test. A two-sided P value of <0.05 was considered statistically significant. For TAA-specificity validation experiments, statistical analysis was performed using unpaired kruskal-wallis test to access overall differences between expansion conditions. Rstudio (2022.07.1) was used with R 4.0.5 to calculate P values and generate plots by use of the tidyverse package suite (51 (link)) cowplot.
Excel 2018
Manufactured by GraphPad
Excel 2018 is a spreadsheet software program that allows users to organize, analyze, and visualize data. It provides a range of features and tools for creating and managing spreadsheets, including functions for calculations, data management, and chart creation.
Automatically generated - may contain errors
5 protocols using excel 2018
1
Tumor-Infiltrating Lymphocyte Expansion Protocol
2
Comparative Analysis of Gene Expression
3
Statistical Analysis of Experimental Data
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Statistical analyses were conducted using Excel 2018 and GraphPad Prism 6.0 software (La Jolla, Ca) with significance set at P < 0.05. Statistical evaluation utilized unpaired Student t-tests or analysis of variance (ANOVA) based on the characteristics of analysis. Data is expressed ± standard error of the mean.
4
Quantitative Cell Imaging and Analysis
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The number of the used animals and independent experiments are labeled in the Figure legends. All macroscopic pictures and microscopic images are representative of the indicated number of independent experiments. Individual data points with mean and SEM or median and interquartile range (IQR) are shown in each diagram. Box plots are shown with whiskers of 10–90 percentiles. Microscopic image processing and analysis were performed using NIS-Elements Imaging Software (Nikon, version BR 4.60.00), Photoshop CS6 (Adobe, version 16.0.0), and NIH Fiji software (versions 1.52i -1.53 f). Flow cytometry result processing and analysis were performed using FCS Express 6 Flow Cytometry Software (De Novo Software, version 6.06.0033. Data processing and statistical analyses were performed using Graphpad Prism (version 7.03) and Excel 2018 software. All datasets were tested for normality using Shapiro–Wilk test of normality. A dataset was considered to be normally distributed with P > 0.01. Normally distributed datasets were tested with Student paired or unpaired two-tailed T-test and non-normally distributed datasets were tested with Wilcoxon signed-rank test. A difference was considered statistically significant at P < 0.05.
5
Analyzing Cell Behavior Assays
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Statistical analysis was done on Microsoft Excel 2018 and GraphPad Prism 8.4.3 software. GraphPad Prism was used to analyse the adherence, invasion, and survival assays by performing a two-way ANOVA at a 95% Confidence Interval (p = 0.05).
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