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Tali image cytometer

Manufactured by Thermo Fisher Scientific
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Sourced in Japan

The Tali™ Image Cytometer is a compact, automated cell analysis instrument designed for cell counting and viability assessment. It utilizes image-based technology to provide accurate and reliable cell data.

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Lab products found in correlation

Evos fl cell imaging system, by Thermo Fisher Scientific (2 mentions) Tali cellular analysis slide, by Thermo Fisher Scientific (2 mentions) Cellrox orange or green reagent, by Thermo Fisher Scientific (2 mentions) 7 aminoactinomycin d 7 aad, by BD (1 mentions) Collagenase nb4, by Serva Electrophoresis (1 mentions) Lsr 2, by BD (1 mentions) 100 μm cell strainer, by BD (1 mentions) Sytox green nucleic acid stain, by Thermo Fisher Scientific (1 mentions) Disposable counting slide, by Thermo Fisher Scientific (1 mentions) Bz x810, by Keyence (1 mentions) Annexin 5 fluos, by Roche (1 mentions) Tali apoptosis kit annexin 5 alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Tryple, by Thermo Fisher Scientific (1 mentions) Chromium controller, by 10x Genomics (1 mentions) Novaseq, by Illumina (1 mentions)

Close spelling variants of this product

Tali image-based cytometer (189 citations) Tali cytometer (2 citations) Tali Image-based Cytometer Apoptosis Kit (2 citations)

12 protocols using tali image cytometer

1

Enzymatic Isolation of Stromal Vascular Fraction

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SVF was isolated enzymatically from lipoaspirate tissue by digestion with collagenase [18 (link)]. Briefly, the aspirate was washed three or four times with lactated Ringer's solution and digested with collagenase NB-4 (SERVA Electrophoresis GmbH) in lactated Ringer's solution in standard tissue culture flasks (BD Falcon). Digestion was performed at 37°C with 5% humidified CO2 and continuous agitation for 60 min. The digest was then centrifuged for 20 min at 400 ×g. The supernatant containing adipocytes was discarded and the pellet containing the SVF was washed twice and filtered through a 100 μm cell strainer (BD Falcon). The SVF cells were counted manually using a Neubauer chamber. Viability was determined by staining with 7-aminoactinomycin D (7-AAD) (BD Biosciences) and flow cytometry analysis (BD LSR II, BD Biosciences). Counting and viability analyses were performed in two replicates for each sample.
Verification of the accuracy of manual counting of SVF was performed by direct comparison of the manual counting method against counts obtained using an automated image based cell counter (Tali Image Cytometer, Life Technologies). Difference between the two methods was found to be not significant (see Supplementary Table 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2015/109353).
SundarRaj S., Deshmukh A., Priya N., Krishnan V.S., Cherat M, & Majumdar A.S. (2015). Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate. Stem Cells International, 2015, 109353.
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2

Oxidative Stress Measurement in MSCs

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MSC-1 and MSC-2 were primed with 1 μg/ml LPS for 4 h at 37 °C, 5% CO2, followed by stimulation with 300 μM BzATP for 30 min. After stimulation, cells were detached and incubated with 10 μM dichlorodihydrofluorescein diacetate (DCFDA) (Invitrogen) for 30 min at 37 °C. Cells were then analyzed using a Tali Image Cytometer (Life Technologies, Monza, Italy) at 458 nm excitation with a 520/525 nm emission filter. MSC-1 and MSC-2 were also incubated with 500 μM H2O2 (Sigma) for 4 h at 37 °C, absorbance of these samples was considered as 100%. Alternatively, G-MDSC and M-MDSC subsets were incubated at 37 °C in 1%FBS in the presence of 2.5 μM DCFDA for 30 min and evaluated by Gallios cytometer.
Bianchi G., Vuerich M., Pellegatti P., Marimpietri D., Emionite L., Marigo I., Bronte V., Di Virgilio F., Pistoia V, & Raffaghello L. (2014). ATP/P2X7 axis modulates myeloid-derived suppressor cell functions in neuroblastoma microenvironment. Cell Death & Disease, 5(3), e1135-.
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3

Cell Viability Assessment with Tali Cytometer

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The viability of the cells before transfer and cells transferred with membrane, re-adhered and after 24 h of culture detached with 0.05 % trypsin–EDTA–ATMP solution was measured using the Tali® Viability Kit—Dead Green on a Tali® Image Cytometer (Life Technologies™).
Kawecki M., Kraut M., Klama-Baryła A., Łabuś W., Kitala D., Nowak M., Glik J., Sieroń A.L., Utrata-Wesołek A., Trzebicka B., Dworak A, & Szweda D. (2016). Transfer of fibroblast sheets cultured on thermoresponsive dishes with membranes. Journal of Materials Science. Materials in Medicine, 27, 111.
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4

UVA-induced ROS Generation in Skin Cells

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HaCaT cells and HGFs were pre-treated with ULH-002 at different concentrations for 2 h. Then, the cells were irradiated with UVA (32 J/cm2). At 2 h after UVA irradiation, cellular ROS generation in cells was detected with the CellROX® Orange or Green Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommended protocol. ROS production in cells was observed by the EVOS® FL Cell Imaging System (Thermo Fisher Scientific). To qualitatively analyze the ROS production, HaCaT cells and HGFs were detached from the culture substratum-surface by 0.025 w/v% trypsin–0.01% EDTA solution and then suspended in PBS (-) at a concentration of 1 × 106 cells/mL. In addition, 25 µL of the cell suspension was infused into a Tali™ Cellular Analysis Slide (T10794, Thermo Fisher Scientific, Tokyo) and analyzed by the Tali™ Image Cytometer (Thermo Fisher Scientific, Tokyo, Japan).
Xiao L., Mochizuki M., Nakahara T, & Miwa N. (2021). Hydrogen-Generating Silica Material Prevents UVA-Ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents. Antioxidants, 10(1), 76.
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5

Live Cell Counting using Image Cytometer

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Live cell numbers were counted using an image-based cytometer (Tali® Image Cytometer; Thermo Fisher Scientific) (Tomaru and Kimura, 2016 ). The SYTOX® Green nucleic acid stain (excitation, 504 nm; emission, 523 nm; Thermo Fisher Scientific) was added to the cell suspension (1% [v/v]) at a final concentration of 0.5‍ ‍μM. A 25-μL aliquot of culture was placed on a disposable counting slide (Thermo Fisher Scientific). Cells were counted according to the manufacturer’s instructions after standing for 10‍ ‍min in the dark at room temperature to allow the cells to settle. The SYTOX® Green stain only permeates cells with compromised plasma membranes, but does not cross the membranes of live cells. Cell counts were performed using the red (excitation filter, 543/22 nm; long-pass emission filter, 585 nm) and green (excitation filter, 466/40 nm; emission filter, 525/50 nm) channels of the image-based cytometer. The cell size range (in a bright field), red fluorescent threshold, green fluorescent threshold, circularity, and sensitivity were set at 3–20‍ ‍μm, 1,200, 1,200, 8, and 9, respectively. Cells with red fluorescence and no green fluorescence were counted as live cells. Cell counts were performed in duplicate for each sample.
Tomaru Y., Yamaguchi H, & Miki T. (2021). Growth Rate-dependent Cell Death of Diatoms due to Viral Infection and Their Subsequent Coexistence in a Semi-continuous Culture System. Microbes and Environments, 36(1), ME20116.
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6

Quantifying Oxidative Stress in HaCaT Cells

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HaCaT cells were cultivated in DDW- or HW-prepared culture medium for 2 h. The medium was then changed to regular medium containing H2O2 at different concentrations. At 2 h after H2O2 treatment, cellular ROS generation in HaCaT cells was detected using the CellROX® Orange or Green Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommended protocol. ROS production in cells was observed by the EVOS® FL Cell Imaging System (Thermo Fisher Scientific). To qualitatively analyze the ROS production, HaCaT cells were detached from the culture substratum-surface using a 0.25 w/v% trypsin, 1 mmol/L EDTA solution, and then suspended in PBS (−) at a concentration of 1 × 106 cells/mL. 25 µL of the cell suspension was infused into a Tali™ Cellular Analysis Slide (T10794, Thermo Fisher Scientific) and analyzed by the Tali™ Image Cytometer (Thermo Fisher Scientific) [19 (link)].
Xiao L, & Miwa N. (2021). Hydrogen Nano-Bubble Water Suppresses ROS Generation, Adipogenesis, and Interleukin-6 Secretion in Hydrogen-Peroxide- or PMA-Stimulated Adipocytes and Three-Dimensional Subcutaneous Adipose Equivalents. Cells, 10(3), 626.
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7

Multimodal Imaging of Lysosomal and Autophagy Dynamics

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MIA PaCa-2 cells were plated in 24-well glass-bottom plates with EMEM + 10% FBS and left overnight to adhere. Then, 50–80% confluent cells were treated with a medium containing a compound for 30 min, followed by treatment with 5 μg/mL acridine orange or 75 nM Lysotracker Red DND-99 for 30 min. The cells were washed twice with PBS and phenol-free DMEM was provided in the presence or absence of 5 μg/mL Hoechst 33532 for nucleus staining. Measurement was performed on BZ-X810 (Keyence). For cell cytometer analysis, Lysotracker-stained cells were trypsinized with 0.25% trypsin–EDTA for 5 min at 37 °C, and washed twice with phenol-free DMEM. Red fluorescence of the cells was measured using Tali Image Cytometer (Thermo Fisher Scientific). PROTEOSTAT staining (ENZ-51035-K100) was performed on MIA PaCa-2 cells 5 days after the treatment with ARL-17477 or CQ according to the manufacturer’s protocol.
Nomura T.K., Endo S., Kuwano T., Fukasawa K., Takashima S., Todo T., Furuta K., Yamamoto T., Hinoi E., Koyama H, & Honda R. (2023). ARL-17477 is a dual inhibitor of NOS1 and the autophagic-lysosomal system that prevents tumor growth in vitro and in vivo. Scientific Reports, 13, 10757.
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8

Cell Cycle Analysis of A549 Cells

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Human A549 cells were seeded in 6-well plates at a density of 1 × 105 cells/well. After 24 h incubation, the culture medium was replaced, and the cells were subjected to specific treatments. Following fixation, the cells were treated with the optimized TaliTM cell cycle reagent (Thermo, Middlesex, MA, USA) and incubated in darkness for 30 min. The Tali® image cytometer (Thermo, Middlesex, MA, USA) was employed for cell cycle analysis. The acquired cell cycle data from the TaliTM image-based cytometer were analyzed both on the instrument and through dedicated cell cycle modeling software.
Lee Y.G., Kim T.H., Kwon J.E., Kim H, & Kang S.C. (2024). Cytotoxic Effects of Ardisiacrispin A from Labisia pumila on A549 Human Lung Cancer Cells. Life, 14(2), 276.
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9

Apoptosis Analysis of Temozolomide-treated Cells

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Cells were transfected with si-TLK1 for 48 h in 6-well plates. After replacing the media, 2.5 mM temozolomide (TMZ; cat. no. T2577-25MG; Sigma-Aldrich; Merck KGaA) was added to each well. After 48 h of incubation with TMZ at 37°C, cells were harvested. The cells were then washed with PBS and centrifuged at 200 × g for 5 min. The cell pellet was suspended with 100 µl of Annexin V-FLUOS (cat. no. 11988549001; Roche Applied Science) labelling solution. Annexin V-FLUOS (20 µl) was initially diluted in 1 ml incubation buffer with 20 µl propidium iodide solution. The suspension was incubated for 10-15 min at 15-25°C. Analysis was performed using a Tali® Image Cytometer (Thermo Fisher Scientific, Inc.). Experiments were performed in triplicate.
Ibrahim K., Murad N.A., Harun R, & Jamal R. (2020). Knockdown of Tousled-like kinase 1 inhibits survival of glioblastoma multiforme cells. International Journal of Molecular Medicine, 46(2), 685-699.
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10

Quantifying UV-induced Apoptosis in HGFs

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UVA-induced apoptosis in HGFs was detected by a Tali Apoptosis Kit - Annexin V Alexa Fluor 488 and propidium iodide (PI) (A10788, Thermo Fisher Scientific, Tokyo, Japan) according to the manufacture’s protocol. After being washed by PBS (-) twice, cells (5 × 105 to 1 × 106 cells/mL) were stained with Annexin V solution for 20 min in the dark. Cells were then stained with PI solution for 1–5 min. The intensity of the green and red fluorescence was measured with the Tali™ Image Cytometer (Thermo Fisher Scientific, Tokyo, Japan).
Xiao L., Mochizuki M., Nakahara T, & Miwa N. (2021). Hydrogen-Generating Silica Material Prevents UVA-Ray-Induced Cellular Oxidative Stress, Cell Death, Collagen Loss and Melanogenesis in Human Cells and 3D Skin Equivalents. Antioxidants, 10(1), 76.
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