Anti human cd163

10⁶ cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat. # 102705, clone 90, FITC, 3 μl, Biolegend, San Diego, CA), anti-mouse CD80 (cat. # 104707, clone 16–10A1, PE, 3 μl, Biolegend, CA), anti-mouse CD206 (cat. # 141708, clone C068C2, APC, 3 μl, Biolegend, CA), anti-human CD80 (cat. # 305208, clone 2D10, PE, 10 μl, BD Pharmingen, CA), anti-human CD86 (cat. # 555660, clone 2331, APC, 10 μl, BD Pharmingen, CA), anti-human CD163 (cat. # 326512, clone RM3/1, PerCP-Cy5.5, 15 μl, BD Pharmingen, CA), anti-human CD206 (cat. # 551135, clone 19.2, FITC, 10 μl, BD Pharmingen, CA). Cell counts were obtained before flow staining by trypan blue exclusion and normalized to single stained and isotype controls on a FACSCanto II (BD Biosciences) or BD Accuri c6 (BD Biosciences) and FlowJo Ver. 10 software (Tree Star, Ashland, OR, USA) or BD FACS Xpress software were used for analyses.