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Anti human cd163

Manufactured by BD

Anti-human CD163 is a cell surface receptor protein that is predominantly expressed on cells of the monocyte/macrophage lineage. It functions as a scavenger receptor, facilitating the clearance of hemoglobin-haptoglobin complexes from the bloodstream.

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2 protocols using anti human cd163

1

Intracellular IFN-α Detection in Monocytes

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Intracellular IFN-α staining was performed according to the intracellular staining protocol from BD Bioscience. Cells were treated with Brefeldin A (GolgiPlug, BD Bioscience, 555029) 6 h prior to harvesting and stained with an anti-human IFN-α antibody (Miltenyi Biotec, 130–092-602). RPE cells and monocyte-derived cells were distinguished by the expression of CD206 (anti-human CD206, biolegend, 321124) or CD163 (anti-human CD163, BD Bioscience, 556018) on monocyte-derived cells as well as CellTracker Violet BMQC (Invitrogen, C10094) staining of monocyte-derived cells prior to co-culturing. Data were acquired on a LSRII flow cytometer (BD Biosciences) and analyzed with FlowJo software (Tree Star).
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2

Macrophage Phenotyping by Flow Cytometry

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106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat. # 102705, clone 90, FITC, 3 μl, Biolegend, San Diego, CA), anti-mouse CD80 (cat. # 104707, clone 16–10A1, PE, 3 μl, Biolegend, CA), anti-mouse CD206 (cat. # 141708, clone C068C2, APC, 3 μl, Biolegend, CA), anti-human CD80 (cat. # 305208, clone 2D10, PE, 10 μl, BD Pharmingen, CA), anti-human CD86 (cat. # 555660, clone 2331, APC, 10 μl, BD Pharmingen, CA), anti-human CD163 (cat. # 326512, clone RM3/1, PerCP-Cy5.5, 15 μl, BD Pharmingen, CA), anti-human CD206 (cat. # 551135, clone 19.2, FITC, 10 μl, BD Pharmingen, CA). Cell counts were obtained before flow staining by trypan blue exclusion and normalized to single stained and isotype controls on a FACSCanto II (BD Biosciences) or BD Accuri c6 (BD Biosciences) and FlowJo Ver. 10 software (Tree Star, Ashland, OR, USA) or BD FACS Xpress software were used for analyses.
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