Nd1 ab74257

Western blotting analysis was carried out as detailed elsewhere (43 (link),44 (link)). Twenty micrograms of total cellular proteins obtained from various cell lines were denatured and loaded on sodium dodecyl sulfate (SDS) polyacrylamide gels. Afterward, the gels were electroblotted onto polyvinylidene difluoride (PVDF) membrane for hybridization. The antibodies used for this investigation were from Abcam [ND1(ab74257), ND5 (ab92624), ND6 (ab81212), CO2 (ab110258), Tom20 (ab56783), p62 (ab56416) and Total OXPHOS Human WB Antibody Cocktail (ab110411)], Proteintech [(CYTB (55090-1-AP), ND2 (19704-1-AP), ATP8 (26723-1-AP), and β-actin (20536-1-AP)], Novus [ND4 (NBP2-47365)], Cell Signaling Technology [LC3A/B (CST,#4108)]. Peroxidase Affini Pure goat anti-mouse IgG and goat anti-rabbit IgG (Jackson) were used as secondary antibodies, and protein signals were detected using the ECL system (CWBIO). Antibodies against above human mtDNA encoding proteins were validated using 143B.TK⁻ cell line and mtDNA-less ρ°206 cell line (Supplemental Figure S3). Quantification of density in each band was performed as described previously (43 (link),44 (link)).

Western blotting was performed as detailed previously (32 (link), 34 (link)). The antibodies used for this investigation were the following: TOM20 (ab56783), ND1 (ab74257), ATP6 (ab101908), and CO2 (subunit II of cytochrome c oxidase) (ab110258) from Abcam; ND4 (sc-20499-R) and ND6 (sc-20667) from Santa Cruz Biotechnology; and CYTB (apocytochrome b) (55090-1-AP) from Proteintech. Peroxidase Affinipure goat anti-mouse IgG and goat anti-rabbit IgG (Jackson) were used as secondary antibodies, and protein signals were detected using an enhanced chemiluminescence (ECL) system (CWbio). Quantification of density in each band was performed as detailed previously (32 (link), 34 (link)).