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Igm 2 41 apc

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The IgM (II/41) APC is a fluorescently-labeled antibody product used for flow cytometry applications. It binds to the IgM surface marker and is conjugated to the APC (Allophycocyanin) fluorescent dye. This product can be used to identify and analyze IgM-positive cells in biological samples.

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Lab products found in correlation

Brdu flow kit, by BD (2 mentions) Imject alum adjuvant, by Thermo Fisher Scientific (2 mentions) Np20 cgg, by Biosearch Technologies (2 mentions) Igg1 a85 1 pe, by BD (2 mentions) Brdu bu20a pe, by Thermo Fisher Scientific (2 mentions) Cd16 cd32 2.4g2, by BD (2 mentions) B220 ra3 6b2 apc efluor780, by Thermo Fisher Scientific (2 mentions) Igd 11 26c fitc, by Thermo Fisher Scientific (2 mentions) Cd86 po3.1 pe, by Thermo Fisher Scientific (2 mentions) Hcd2 rpa 2.10 apc, by Thermo Fisher Scientific (2 mentions) Cxcr4 l276f12 apc, by BioLegend (2 mentions) Cd86 gl1 percp cy5, by BioLegend (2 mentions) κ 187.1 fitc, by BD (2 mentions) Cd19 1d3 apc cy7, by BD (2 mentions) Fas jo2 pe cy7, by BD (2 mentions) Igg1 a85 1 fitc, by BD (2 mentions) Ebioscience cell proliferation dye efluor670, by Thermo Fisher Scientific (2 mentions) Gl7 fitc, by BD (2 mentions) Facscanto, by BD (2 mentions) B220 ra3 6b2 apc, by Thermo Fisher Scientific (1 mentions)

5 protocols using igm 2 41 apc

1

Immunization and BrdU Incorporation Assay

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Mice were immunized i.p. or i.v. with 100μl of PBS-washed citrated SRBCs (Colorado Serum Company) or i.p. with 50μg NP20-CGG (Biosearch Technologies) plus Imject Alum Adjuvant (ThermoFisher Scientific) in 100μl PBS. For SRBC-specific antibody titers, serum was collected on indicated days and bound to SRBCs and detected with IgM (II/41) APC (Thermo Fisher Scientific) and IgG1 (A85–1) PE (BD Biosciences) by flow cytometry. For BrdU pulse experiments, mice were injected i.p. with 2mg BrdU in 200μl PBS and sacrificed 2 h later. Incorporation of BrdU was detected using the BrdU Flow Kit (BD Biosciences) according to manufacturer’s instructions and BrdU (BU20A) PE (Thermo Fisher Scientific).
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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2

Multiparameter Flow Cytometry Analysis

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Single cell suspensions from tissues were ACK lysed and stained in 1% FBS in PBS containing 0.05% sodium azide. Cells were gated according to size and granularity based on FSC-A and SSC-A. Doublets were excluded by FSC-A versus FSC-H gating. Non-antigen-specific binding was blocked with CD16/CD32 (2.4G2) (BD Biosciences). The following antibodies were used for staining: B220 (RA3–6B2) APC-eFluor780, IgM (II/41) APC, IgD (11–26c) FITC, CD86 (PO3.1) PE, hCD2 (RPA-2.10) APC (Thermo Fisher Scientific), CXCR4 (L276F12) APC, CD86 (GL1) PerCP Cy5.5 (BioLegend), κ (187.1) FITC, CD19 (1D3) APC-Cy7, FAS (Jo2) PE-Cy7, GL7 FITC and IgG1 (A85–1) FITC (BD Biosciences). Cell proliferation was analyzed using the eBioscience Cell Proliferation Dye eFluor670 (Thermo Fisher Scientific). Samples were acquired on a FACSCanto (BD Biosciences) and analyzed with Flowjo (Becton, Dickinson and Company).
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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3

Multiparameter Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Single cell suspensions from tissues were ACK lysed and stained in 1% FBS in PBS containing 0.05% sodium azide. Cells were gated according to size and granularity based on FSC-A and SSC-A. Doublets were excluded by FSC-A versus FSC-H gating. Non-antigen-specific binding was blocked with CD16/CD32 (2.4G2) (BD Biosciences). The following antibodies were used for staining: B220 (RA3–6B2) APC-eFluor780, IgM (II/41) APC, IgD (11–26c) FITC, CD86 (PO3.1) PE, hCD2 (RPA-2.10) APC (Thermo Fisher Scientific), CXCR4 (L276F12) APC, CD86 (GL1) PerCP Cy5.5 (BioLegend), κ (187.1) FITC, CD19 (1D3) APC-Cy7, FAS (Jo2) PE-Cy7, GL7 FITC and IgG1 (A85–1) FITC (BD Biosciences). Cell proliferation was analyzed using the eBioscience Cell Proliferation Dye eFluor670 (Thermo Fisher Scientific). Samples were acquired on a FACSCanto (BD Biosciences) and analyzed with Flowjo (Becton, Dickinson and Company).
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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4

Multicolor Immunofluorescence Analysis of Mouse Spleen

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Spleens embedded in Tissue TEK O.C.T. (Sakura Finetek) were sectioned on a Microtome Cryostat HM 505 E (Microm). Sections were fixed with acetone and blocked with 5% FBS in PBS. Florescent images were acquired on an Axio Imager.M1 (Zeiss) microscope equipped with an Orca-ER (Hamamatsu) camera. SlideBook (3i) was used as the imaging software. Gimp (GNU Image Manipulation Program) was used for image editing. The following antibodies were used: B220 (RA3-6B2) APC, IgM (II/41) APC, IgD (11-26c) PE (Thermo Fisher Scientific), Moma1 Biotin (Abcam), and Streptavidin-Cy3 (Jackson ImmunoResearch). For immunohistochemical analysis, spleens were formalin-fixed and paraffin-embedded. B220 (RA3-6B2) Biotin (BD Biosciences) and Myc (Y69) (Abcam) and were used as primary antibodies and horseradish-peroxidase-coupled Streptavidin or anti-Rabbit IgG were used as secondary antibodies. TUNEL staining was performed by using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (MilliporeSigma) according to the manufacturer’s protocol.
Ramezani-Rad P., Leung C.R., Apgar J.R, & Rickert R.C. (2020). E3 ubiquitin ligase Fbw7 regulates the survival of mature B cells. Journal of immunology (Baltimore, Md. : 1950), 204(6), 1535-1542.
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5

Immunization and BrdU Incorporation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mice were immunized i.p. or i.v. with 100μl of PBS-washed citrated SRBCs (Colorado Serum Company) or i.p. with 50μg NP20-CGG (Biosearch Technologies) plus Imject Alum Adjuvant (ThermoFisher Scientific) in 100μl PBS. For SRBC-specific antibody titers, serum was collected on indicated days and bound to SRBCs and detected with IgM (II/41) APC (Thermo Fisher Scientific) and IgG1 (A85–1) PE (BD Biosciences) by flow cytometry. For BrdU pulse experiments, mice were injected i.p. with 2mg BrdU in 200μl PBS and sacrificed 2 h later. Incorporation of BrdU was detected using the BrdU Flow Kit (BD Biosciences) according to manufacturer’s instructions and BrdU (BU20A) PE (Thermo Fisher Scientific).
Ramezani-Rad P., Chen C., Zhu Z, & Rickert R.C. (2020). Cyclin D3 Governs Clonal Expansion of Dark Zone Germinal Center B Cells. Cell reports, 33(7), 108403.
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