Rectangular reaction chambers were constructed by gently pressing a rectangular polydimethylsiloxane (PDMS) ring onto a glass slide. To prepare the mold for making the PDMS ring, rectangular patterns were cut out of a 6.35-mm-thick acrylic sheet using a laser cutter (Epilog Laser) and then glued onto the bottom surface of a Petri dish using a two-part epoxy (Gorilla Epoxy; Gorilla Glue Inc.). PDMS rings were prepared using a 10:1 weight mixture of base to curing agent (Sylgard 184; Dow Corning). Before being introduced into the reaction chamber, hydrogel solution of 10% acrylamide and 0.1% bis-acrylamide (Amersco) was degassed for 30 min in a vacuum desiccator to completely remove dissolved oxygen, and cotton wires prewetted with hydrogel solution were placed into the reaction chamber as a way to introduce differential growth. Polymerization was then initiated by introducing tetramethylethylenediamine (Teknova) and ammonium persulfate (Promega) into the gel solution with final concentrations of 0.3% and 0.05%, respectively. The gel solution of a desired volume was immediately pipetted into the polymerization chamber with minimal disturbance so as to avoid introducing oxygen. The solution was allowed to react for 1 h before disassembling the reaction chamber to release hydrogel structures.
Ammonium persulfate
Manufactured by Promega
Sourced in United States
Ammonium persulfate is a chemical compound commonly used as an oxidizing agent and initiator in various applications, including polymerization reactions, electroplating processes, and analytical techniques. It is a colorless crystalline solid that is highly soluble in water. Ammonium persulfate is a versatile laboratory reagent, but its specific applications and usage should be determined by the user based on their research needs and applicable safety protocols.
Automatically generated - may contain errors
2 protocols using ammonium persulfate
1
Hydrogel Synthesis in Rectangular Chambers
2
SDS-PAGE Biochemical Analysis Protocol
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Sodium dodecyl sulfate (SDS) of high purity (>99 % C12 alkyl sulfate) was from Pierce (Rockford, IL, USA), and electrophoresis equipment were from Hoefer Scientific Instruments (San Francisco, CA, USA). N,N'-methylene-bisacrylamide, acrylamide, ammonium persulfate, TEMED and alkaline phosphatase substrates (nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate) were from Promega (Madison, WI, USA). Molecular mass markers for SDS-polyacrylamide gel electrophoresis and nitrocellulose sheets (0.22 μm pores, 100 % nitrocellulose) were from Amersham (Buckinghamshire, UK). Prolong Gold AntiFade Mountant with was from Life Technologies (Carlsbad, CA, USA). Paraformaldehyde and glutaraldehyde EM grade were from TAAB (Reading, UK). All other chemicals were obtained from Sigma (St. Louis, MO, USA).
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