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6 protocols using aluminum chloride

1

HPLC-Based Antioxidant Evaluation

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HPLC solvents including methanol (≥ 99.9%), water (≥ 100%), and acetonitrile (≥ 99.9%) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Folin-Ciocalteu reagent, gallic acid (3,4,5-trihydroxybenzoic acid, ≥ 99%), kaempferol (≥ 97%), quercetin (≥ 95%), DPPH (≥ 100%), ABTS·+ (≥ 100%), trolox (≥ 97%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). methanol (≥ 99%; Duksan, Gyeonggi-do, Korea), Na2CO3 (≥ 99.9%; Duksan), ethanol (≥ 99.9%; Samchun, Pyeongtaek, Gyeonggi-do, Korea), aluminum chloride (98%; Junsei, Japan), and potassium acetate (≥ 99%; Duksan) were also used.
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2

Comprehensive Phytochemical Profiling and Antioxidant Evaluation

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Ethanol was supplied by Ethanol Supplies World Co. (Jeonju, Republic of Korea). Diethyl ether, n-butanol, methyl alcohol, anhydrous ethyl alcohol (99.9%), sodium carbonate, sodium hydroxide, acetic acid, citric acid, hydrochloric acid, and oxalic acid were purchased from Daejung Chemicals & Metals Co. (Siheung, Republic of Korea). 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu reagent, carbazole, galacturonic acid, gallic acid, and catechin were supplied by Sigma-Aldrich (St. Louis, MO, USA). Ascorbic acid was purchased from Reagent Duksan (Ansan, Republic of Korea). Aluminum chloride and sodium nitrite were obtained from Junsei Chemical (Tokyo, Japan). Ginsenosides standards such as Rg1, Re, Rf, Rg2, Rb1, Rc, Rb2, F1, Rd, F2, and Rg3 for HPLC analysis were supplied by Ambo Institute (Daejeon, Republic of Korea).
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3

Quantification of Oxidative Stress Biomarkers

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Dimethylnitrosamine (DMN), hydroxyproline, p-dimethylaminobenzaldehyde, 1,1,3,3-tetraethoxypropane (TEP), chloramine-T, potassium chloride (KCl), Folin-Ciocalteu's phenol reagent, and hydrochloric acid (HCl) were purchased from Sigma (St. Louis, MO). Thiobarbituric acid (TBA) was purchased from Lancaster Co. (Lancashire, UK). Histofine was purchased from Nichirei Biosciences (Tokyo, Japan). Sodium carbonate was purchased from Kanto perchloric acid and aluminum chloride was purchased from Junsei Chemical (Tokyo, Japan).
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4

Bioactive Compounds in Korean Turmeric

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Sliced and dried turmeric (cultivated in Jindo-gun, Republic of Korea, and harvested in October–December 2017) was purchased from Bibong Herb Co. (Yangju-si, Republic of Korea). Food-grade 70% ethanol was purchased from Ethanol Supplies World Co. (Jeonju-si, Republic of Korea), and methanol, sodium carbonate, sodium hydroxide, and hydrochloric acid were purchased from Daejung Chemicals & Metals Co. (Siheung-si, Republic of Korea). Folin–Ciocalteu’s phenol reagent, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2′-azobis(2-amidino-propane) dihydrochloride (AAPH), 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid, catechin, and ascorbic acid were purchased from Sigma Aldrich Co. (St. Louis, MO, USA). Aluminum chloride and sodium nitrite were purchased from Junsei Chemical Co., LTD (Tokyo, Japan).
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5

Comprehensive Analysis of Turmeric Bioactive Compounds

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Dried turmeric (moisture content 15.7 ± 0.1%, crude fat 6.4 ± 0.1, crude protein 7.7 ± 0.1, ash 6.5 ± 0.4) was kindly donated from Bibong Herb (Yangju-si, Korea) and kept in a deep freezer before use. Fermented ethanol, an extraction solvent, was provided by ethanol Supplies World Co. (Jeonju-si, Korea). Methyl alcohol, sodium carbonate, sodium hydroxide, and hydrochloric acid were purchased from Daejung Chemicals & Metals Co. (Siheung-si, Korea). Folin-Ciocalteu’s phenol reagent, 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,2′-azobis (2-amidino-propane) dihydrochloride (AAPH), 1,1-diphenyl-2-picrylhydrazyl (DPPH), gallic acid, catechin, ascorbic acid, HPLC analytical standard curcumin, demethoxycurcumin, bis-demethoxycurcumin, ferulic acid, and 2-Methoxy-4-vinylphenol were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Aluminum chloride and sodium nitrite were purchased from Junsei Chemical Co., LTD (Tokyo, Japan). HPLC grade water, acetonitrile, and methanol were purchased from Honeywell Burdick & Jackson Inc. (Charlotte, NC, USA).
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6

Antioxidant Potential of Persimmon Leaves

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The mature persimmon leaves were taken from the persimmon experiment station, Sangju, South Korea on May 25 (flowering time) and June 25 (fruiting time) in 2016. The 35 trees were tagged to collect leaf samples and the cultivar used was "Sangjudungsi."
The following chemicals were used for analysis: Folin-Ciocalteu reagent, gallic acid, and catechin were obtained from Sigma Chemicals (St Lous, MO, USA). 2, 2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (DPPH) and 2, 2-diphenyl-1-picrylhydrazyl (ABTS) were purchased from TCI Co., Ltd. (Tokyo, Japan). Sodium nitrite, sodium carbonate, and aluminum chloride were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Methanol, ethanol, and sodium hydroxide were obtained from Duksan Pure Chemicals Co. Ltd. (Ansan, Korea). Vanillin was purchased from Samchun Chemical Co., Ltd. (Seoul, Korea). All other chemicals were analytical grade.
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