MiR-NC, miR-218 mimic, anti-MiR-NC, and anti-miR-218 were purchased from Ambion (Austin, TX, USA). Small interfering RNA-218 (siRNA-218) targeting survivin and the siRNA control were purchased from GenePharma (Shanghai, People's Republic of China). The miR-218 mimic, inhibitor, or control were diluted in Opti-MEM medium (Life Technologies, CA, USA) at room temperature (RT) for 15 mins, then transfected human CC cells with miR-218 mimic or inhibitor and cultured for 48 hrs. The expression of miR-218 was examined by qRT-PCR assay. siRNA transfection was performed with Lipofectamine RNAiMAX reagent (Invitrogen, CA, USA) following the manufacturer’s instructions. The expression of survivin was examined by qRT-PCR and western assay.
Mir 218 mimic
Manufactured by Thermo Fisher Scientific
Sourced in United States
The MiR-218 mimic is a laboratory reagent designed to mimic the function of the microRNA miR-218. MicroRNAs are small non-coding RNA molecules that play a regulatory role in gene expression. The MiR-218 mimic can be used in experimental settings to study the biological effects of miR-218 in cell-based assays or other research applications.
Automatically generated - may contain errors
Lab products found in correlation
Opti mem medium, by Thermo Fisher Scientific (1 mentions)
Anti mir nc, by Thermo Fisher Scientific (1 mentions)
Mir nc, by Thermo Fisher Scientific (1 mentions)
Anti mir 218, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
2 protocols using mir 218 mimic
1
Investigating miR-218 and Survivin in Cancer
2
Lung Cancer Cell Transfection with miR-218
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Lung cancer cells were grown in antibiotic-free medium at 70–80% confluence in 6-well plates. Indicated cells were transfected with miR-218 mimic (Ambion), miR-218 inhibitor (Ambion), or negative control (Ambion) using Lipofectamine 2000 transfection reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
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