HCT116 p53+/+ and p53−/− cell lines were obtained from the Vogelstein Laboratory (Johns Hopkins University School of Medicine, Baltimore). LoVo shScr and shp53 were generated by transducing the parental model with retroviral pSUPER vectors expressing control or p53 short hairpin RNA under puromycin selection (0.5 µg/mL). HCT116 BAX/BAK DKO cells were obtained from Professor Markus Rehm (University of Stuttgart, Germany). HCT116 caspase-8 CRISPR cells were obtained from Professor Galit Lahav (Department of Systems Biology, Harvard Medical School, Boston, MA)48 . All HCT116-derived cell lines were cultured in McCoy’s 5A Modified Medium (ATCC, LGC Standards, Middlesex, UK) supplemented with 10% fetal bovine serum (Invitrogen, Carlsbad, CA). LoVo cells were cultured in Dulbecco’s modified Eagle’s medium (ATCC, LGC Standards, Middlesex, UK) with 10% fetal bovine serum, at 37 °C in a humidified atmosphere of 5% CO2. Cell lines in culture were tested at least monthly for Mycoplasma using the Lonza MycoAlert™ kit.
Mccoy s 5a modified medium
Manufactured by American Type Culture Collection
Sourced in United States
McCoy's 5a Modified Medium is a cell culture medium formulated to support the growth and maintenance of various cell lines. It is a modification of the original McCoy's 5a Medium, which is a widely used basal medium for culturing a range of mammalian cell types. The formulation provides the necessary nutrients and supplements to facilitate cell proliferation and viability in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (9 mentions)
Fetal bovine serum (fbs), by American Type Culture Collection (6 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (5 mentions)
Skov3, by American Type Culture Collection (4 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Hct116, by American Type Culture Collection (3 mentions)
Skbr3, by American Type Culture Collection (3 mentions)
Leibovitz s l 15 medium, by American Type Culture Collection (3 mentions)
β mercaptoethanol, by Merck Group (3 mentions)
T24 bladder cancer cells, by American Type Culture Collection (2 mentions)
Sv huc 1, by American Type Culture Collection (2 mentions)
Ovcar3, by American Type Culture Collection (2 mentions)
Rpmi 1640 medium, by American Type Culture Collection (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Mda mb 453, by American Type Culture Collection (2 mentions)
F 12k medium, by American Type Culture Collection (2 mentions)
Ht 29 cell, by American Type Culture Collection (2 mentions)
Ht 29, by American Type Culture Collection (2 mentions)
Pen strep, by Thermo Fisher Scientific (2 mentions)
Glutamax, by Thermo Fisher Scientific (2 mentions)
32 protocols using mccoy s 5a modified medium
1
Isogenic HCT116 and LoVo Cell Lines
2
Prostate and Bladder Cancer Cell Culture
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VCaP, LNCaP, and NCI‐H660 prostate cancer cells, T24 bladder cancer cells, McCoy's 5 A Modified Medium, and fetal bovine serum (FBS) were purchased from ATCC (Manassas, VA). DMEM and RPMI‐1640 cell culture media and glutamine were purchased from Life technologies (Carlsband, CA). VCaP cells were maintained in DMEM supplemented with 10% FBS. LNCaP cells were maintained in RPMI‐1640 supplemented with 10% FBS, 2.8 mM l ‐glutamine. NCI‐H660 cells were maintained in RPMI‐1640 medium supplemented with 5% FBS, 4 mM l ‐glutamine, 0.005 mg/ml insulin, 0.01 mg/ml transferrin, 30 nM sodium selenite, 10 nM β‐estradiol, and 10 nM hydrocortisone. T24 bladder cancer cells were maintained in McCoy's 5 A Modified Medium, supplemented with 5% FBS. All cells were cultured in a 5% CO2 humidified incubator at 37°C.
3
Characterization of Bladder Cancer Cell Lines
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Four bladder cancer cell lines, J82 (ATCC® HTB-1™), HT-1376 (ATCC® CRL-1472™), RT4 (ATCC® HTB-2™), and T24 (ATCC® HTB-4™) and a normal human urothelial cell line, SV-HUC-1 (ATCC® CRL-9520™), was obtained from ATCC (Manassas, VA, USA). J82 and HT-1376 cell lines were cultured in Eagle's Minimum Essential Medium (EMEM, Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS) (Sigma). RT4 and T24 cells were cultured in McCoy's 5a Modified Medium (ATCC, Catalog No. 30-2007) supplemented with 10% FBS. All cells were cultured at 37°C with 5% CO2.
4
Immortalized Human Ovarian Cell Lines
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Human ovarian surface epithelial cells (IOSE25) were immortalized and cultured as previously described (34 (link)). Four human ovarian cancer cell lines, A2780, OVCAR3, SKOV3 and 3AO were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and the American Type Culture Collection (ATCC; Manassas, VA, USA), respectively.
The ovarian cancer cell lines were maintained according to the vendor's instructions. Briefly, A2780 and 3AO cell lines were routinely cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA). SKOV3 cells were cultured in McCoy's 5A Modified Medium (ATCC) with 10% FBS (Gibco). OVCAR3 cells were cultured in RPMI-1640 medium (ATCC) with 20% FBS (Gibco). All the media contained 1% penicillin-streptomycin (100 U/ml penicillin and 100 µg/ml streptomycin). The ovarian cancer cells were cultured and maintained in a humidified incubator at 37°C and supplemented with 5% CO2.
The ovarian cancer cell lines were maintained according to the vendor's instructions. Briefly, A2780 and 3AO cell lines were routinely cultured in Dulbecco's modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA). SKOV3 cells were cultured in McCoy's 5A Modified Medium (ATCC) with 10% FBS (Gibco). OVCAR3 cells were cultured in RPMI-1640 medium (ATCC) with 20% FBS (Gibco). All the media contained 1% penicillin-streptomycin (100 U/ml penicillin and 100 µg/ml streptomycin). The ovarian cancer cells were cultured and maintained in a humidified incubator at 37°C and supplemented with 5% CO2.
5
HCT116 Cell Line Cultivation and Transfection
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HCT116 cell line was purchased from the American Tissue Culture Collection (ATCC,
cat. no. ATCC CCL-247) and the hRpn13-deletion (ΔhRpn13) HCT116 cell line
generated in this study, as described below. Cells were grown in McCoy’s
5A modified medium (ATCC), supplemented with 10% fetal bovine serum
(Atlanta Biologicals) in a 37 °C humidified atmosphere of 5%
CO2. Plasmids were transfected using Lipofectamine LTX according
to the manufacturer’s instructions (Thermo Fisher Scientific).
cat. no. ATCC CCL-247) and the hRpn13-deletion (ΔhRpn13) HCT116 cell line
generated in this study, as described below. Cells were grown in McCoy’s
5A modified medium (ATCC), supplemented with 10% fetal bovine serum
(Atlanta Biologicals) in a 37 °C humidified atmosphere of 5%
CO2. Plasmids were transfected using Lipofectamine LTX according
to the manufacturer’s instructions (Thermo Fisher Scientific).
6
Culturing Human Osteosarcoma Cell Lines
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Human osteosarcoma cell lines U-2OS, MG63, and SAOS and osteoblastic cell line hFOB1.19 were purchased from the Type Culture Collection of the Chinese Academy of Sciences. The U-2OS, MG63, and SAOS cells were cultured in Eagle's minimum essential medium (ATCC) supplemented with 10% FBS (Thermo Fisher Scientific, Inc.). hFOB1.19 cells were cultured in McCoy's 5a modified medium (ATCC) containing 10% FBS. All cells were cultured at 37°C with 5% CO2.
7
Breast Cancer Cell Lines Protocol
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DNA samples from five human breast cancer cell lines, SK-BR-3, MDA-MB-231, MDA-MB-361, MDA-MB-453, and BT-474, which were used to prepare components A, B, C, D, and E, respectively. The cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA) as frozen stocks and cultured in the NIST laboratory using standard cell culture methods. MDA-MB-231, MDA-MB-361, and MDA-MB-453 cells were cultured in Leibovitz's L-15 Medium (ATCC # 30-2008) supplemented with 10% fetal bovine serum (FBS, Gibco # 10437-028, except MDA-MB-361 where 20% FBS was used) at 37 °C in an air atmosphere without added CO2. SK-BR-3 cells were grown in the McCoy’s 5A modified medium (ATCC # 30-2007) supplemented with 10% FBS at 37 °C in a humidified (5% CO2, 95% air) atmosphere. BT-474 cells were cultured in the Hybri-Care Medium (ATCC # 46-X) supplemented with 1.5 g/L sodium bicarbonate and 10% FBS at 37 °C in a humidified (5% CO2, 95% air) atmosphere.
8
Culturing Human Ovarian Cancer Cell Lines
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Human OC cell lines ES2 and SKOV3 and human normal ovarian surface epithelial cells, IOSE80, were purchased from the ATCC (Manassas, VA, USA) and cultured in McCoy's 5a Modified Medium (ATCC), McCoy's 5a Modified Medium and DMEM (Gibco, El Paso, TX, USA), respectively. Human OC cell line HO8910 was purchase from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and was cultured in DMEM (Gibco). All mediums were supplemented with 10% (v/v) FBS (Sigma, St. Louis, MO, USA), 100 IU/mL penicillin (Baomanbio, Shanghai, China) and 100 mg/mL streptomycin (Baomanbio). All cell lines were cultured at 37°C in a humidified atmosphere containing 5% CO2.
9
In Vitro Bladder Cancer Cell Lines
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Human T24 (urothelial grade III BC) and SV-HUC-1 (immortalized and non-tumorigenic urothelial cells) cell lines were used in the present study. They were acquired from the American Type Culture Collection (ATCC, Rockville, MD, USA). T24 and SV-HUC-1 cell lines were maintained in McCoy’s 5a Modified Medium and F-12K Medium (ATCC, Rockville, MD, USA), respectively. As a representative in vitro model of canine BC, K9TCC-PU-NK and RDSVS-TCC1 cell lines (both derived from invasive grade III urothelial bladder tumors) were also involved in this study. K9TCC-PU-NK and RDSVS-TCC1 cell lines were generous gifts from Deepika Dhawan (Purdue University College of Veterinary Medicine, USA) and Maciej Parys (Royal (Dick) School of Veterinary Studies and The Roslin Institute, University of Edinburgh, Scotland), respectively. Both canine BC cell lines were cultured in Roswell Park Memorial Institute (RPMI) 1640 Medium (Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wrocław, Poland).
All culture media used in our study were supplemented with 2 mM L-glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin (Sigma-Aldrich, Steinheim, Germany), and 10% heat-inactivated fetal bovine serum (FBS, Gibco, Grand Island, NY, USA). All cell cultures were performed at 37 °C in a humidified atmosphere containing 5% CO2.
All culture media used in our study were supplemented with 2 mM L-glutamine, 100 U/mL penicillin, 100 µg/mL streptomycin (Sigma-Aldrich, Steinheim, Germany), and 10% heat-inactivated fetal bovine serum (FBS, Gibco, Grand Island, NY, USA). All cell cultures were performed at 37 °C in a humidified atmosphere containing 5% CO2.
10
Culturing Human Rhabdoid Tumor G401 Cells
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The human rhabdoid tumor G401 cell line was purchased from the American Type Culture Collection (cat. no. CRL-1441; ATCC, Manassas, VA, USA) and cultured in complete medium which contained ATCC-formulated McCoy's 5A modified medium (cat. no. 30-2007; ATCC) supplemented with 10% fetal bovine serum (FBS; cat. no. 10438026; Thermo Fisher Scientific, Inc.), 100 U/ml penicillin and 0.1 mg/ml streptomycin. Cells were incubated in a cell culture incubator (cat. no. 3308; Thermo Fisher Scientific, Inc.) with a humidified atmosphere containing 5% CO2 at 37°C. An inverted microscope at ×40 magnification (Olympus, Tokyo, Japan) was used to observe cells and to capture images. When cells achieved 90–100% confluency, cells were digested by 0.25% trypsin in 0.53 mM EDTA solution and centrifuged at 100 × g for 5 min at room temperature. Cultured medium was replaced every other day or according to the experimental design.
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