MCPyV-positive, WaGa and MKL-1, and -negative, UM-MCC 34, MCC cell lines have been previously described (Schrama et al. 2019 (link); Fan et al. 2018 (link)). All the cell lines were cultured under standard conditions (37 °C, 5% CO2) in RPMI 1640 medium (PAN-Biotech, Aidenbach, Germany) supplemented with 10% fetal bovine serum (FBS; PAN-Biotech) and 1% penicillin–streptomycin (P/S; PAN-Biotech). Primary skin fibroblasts isolated from healthy skin (F1.15) were cultured in DMEM medium (PAN-Biotech) and DMEM/F12 (1:1) medium (Lonza, Cologne, Germany), supplemented with 10% FBS and 1% P/S under standard conditions (Fan et al. 2021 (link)). The cell lines were regularly tested to ensure the absence of mycoplasma and their identity was verified by DNA finger printing.
Dmem f12 1 1 medium
Manufactured by Lonza
Sourced in Germany, United States
DMEM/F12 (1:1) medium is a cell culture medium commonly used for the in vitro cultivation of a variety of cell types. It is a 1:1 mixture of Dulbecco's Modified Eagle's Medium (DMEM) and Ham's F-12 Nutrient Mixture, providing a balanced formulation of nutrients, vitamins, and other components required for cell growth and maintenance.
Automatically generated - may contain errors
Lab products found in correlation
Sk br 3, by Thermo Fisher Scientific (2 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Mda mb 231, by American Type Culture Collection (2 mentions)
Mcf 7, by American Type Culture Collection (2 mentions)
Mcf 10a, by American Type Culture Collection (2 mentions)
Skbr3, by American Type Culture Collection (2 mentions)
Mcf 10a, by Lonza (2 mentions)
Rpmi 1640 medium, by PAN Biotech (1 mentions)
Dmem medium, by PAN Biotech (1 mentions)
Penicillin streptomycin p s, by PAN Biotech (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Insulin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Cholera toxin, by Merck Group (1 mentions)
Epidermal growth factor (egf), by Merck Group (1 mentions)
Dmem medium, by Lonza (1 mentions)
Foetal bovine serum (fbs), by GE Healthcare (1 mentions)
Rpmi 1640 medium, by Lonza (1 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)
5 protocols using dmem f12 1 1 medium
1
Culturing MCC Cell Lines and Fibroblasts
2
Breast Cell Line Culturing Protocol
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A human breast epithelial cell line (MCF10A) and breast cancer cell lines (MDA-MB-231, MCF7, and SK-BR3) were purchased from the American Type Culture Collection (ATCC, Manassas, VA). MCF10A cells were maintained in Dulbecco Modified Eagle medium (DMEM)/F-12 (1:1) medium (Lonza, Walkersville, MD) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY), 10 ng/ml epidermal growth factor (Sigma-Aldrich Co., St. Louis, MO), 0.5 μg/ml hydrocortisone (Sigma–Aldrich), 100 ng/ml cholera toxin (Sigma-Aldrich), and 10 μg/ml insulin (Sigma–Aldrich). MDA-MB-231, MCF7, and SK-BR3 cells were maintained in DMEM (Gibco) supplemented with 10% FBS.
3
Culturing Pancreatic and Breast Cell Lines
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PANC-1 and MCF-10A cell lines were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in a constant humidity incubator containing 5% CO2 at 37 °C. The PANC-1 human pancreatic cancer cell line was cultured in DMEM medium (LONZA) supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/mL) and streptomycin (0.1 mg/mL). The MCF-10A cell line was cultured in DMEM/F-12 (1:1) medium (LONZA) supplemented with 10% horse serum, 20 ng/mL epidermal growth factor, 0.5 μg/mL hydrocortisone, 10 μg/mL insulin, 1% NEAA, penicillin (100 U/mL) and streptomycin (0.1 mg/mL). The Panc02 mouse pancreatic cancer cell line was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in the Roswell Park Memorial Institute (RMPI), supplemented with 10% FBS, penicillin (100 U/mL) and streptomycin (0.1 mg/mL).
4
Tissue Samples and Cell Lines for OSCC Research
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Tissues from OSCC patients and normal specimens were obtained from patients immediately after surgical resection. The samples were flash frozen in liquid nitrogen and stored at −80 °C until use. All specimens were randomly selected from the Second Affiliated Hospital of Harbin Medical University, China. The Hacat cells and human OSCC cell lines Tca8113 and SCC4 were stored in our laboratory (Laboratory of Medical Genetics, Harbin Medical University); CAL-27 cells were kindly provided by Dr. Hongchen Sun (School of Stomatology, Jilin University), HN-6 and SCC9 cells by Dr. Chao Liu (Nanjing Medical University), and SCC15 cells by Dr. Xiaozhi Liu (Department of Neurosurgery, the Fifth Central Hospital of Tianjin). The human OSCC cell line Tca8113 was cultured in RPMI-1640 medium (Lonza). Hacat, SCC4, SCC15 and CAL-27 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Lonza, Walkersville, MD, USA). The human OSCC cell lines HN-6 and SCC9 were cultured in DMEM/F12 (1:1) medium (Lonza). All cells were supplemented with 10% foetal bovine serum (FBS) (PAA Laboratories GmbH, Pasching, Australia) and cultured at 37 °C in a humidified 5% CO2 atmosphere.
5
Breast Cancer Cell Line Characterization
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The normal breast epithelial cells MCF10A and the breast cancer cell lines MDA-MB-231 (basal type, TNBC), MCF7 (luminal-A type, estrogen receptor-positive breast cancer), SK-BR3 (HER2-positive breast cancer), T47D (luminal-A type, estrogen receptor-positive breast cancer), BT474 (estrogen receptor-positive breast cancer), and BT20 (TNBC) were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA; 15-6, 15-7). MCF10A was maintained in Dulbecco's modified Eagle's medium (DMEM)/F-12 (1:1) medium (Lonza, Walkersville, MD, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 10 ng/mL epidermal growth factor, 0.5 µg/mL hydrocortisone, 100 ng/mL cholera toxin, and 10 µg/mL insulin. SK-BR3 was maintained in DMEM (Gibco) supplemented with 10% FBS.
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