Log In Sign up
The largest database of trusted experimental protocols

Protein disulfide isomerase

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in United States

Protein-disulfide isomerase (PDI) is an enzyme that catalyzes the formation, breakage, and rearrangement of disulfide bonds in proteins. It plays a key role in the proper folding of proteins within the endoplasmic reticulum.

Automatically generated - may contain errors

Lab products found in correlation

Tunicamycin, by Merck Group (1 mentions) Compound c, by Merck Group (1 mentions) Mannitol, by Merck Group (1 mentions) Gsk0660, by Bio-Techne (1 mentions) Griess reagent nitrite measurement kit, by Cell Signaling Technology (1 mentions) A23187, by Merck Group (1 mentions) Antibody against β actin, by Santa Cruz Biotechnology (1 mentions) Dihydroethidium dhe, by Merck Group (1 mentions) Sodium nitroprusside, by Merck Group (1 mentions) Horseradish peroxidase hrp conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Grp78, by Cell Signaling Technology (1 mentions) Gw501516, by Merck Group (1 mentions) Ire1α, by Cell Signaling Technology (1 mentions) L 012, by Fujifilm (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Poly adp ribose polymerase 1 parp1, by Proteintech (1 mentions) Vinculin, by Cell Signaling Technology (1 mentions) Chemidoc mp imaging system, by Bio-Rad (1 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (1 mentions) Aryl hydrocarbon receptor, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-protein disulfide isomerase (2 citations)

4 protocols using protein disulfide isomerase

1

Molecular Mechanisms of Metabolic Dysfunction

Check if the same lab product or an alternative is used in the 5 most similar protocols
PCB2, phenylephrine (Phe), acetylcholine (ACh), sodium nitroprusside (SNP), 1H-Oxadiazolo [ [3 (link),4 (link)],-a] quinoxalin-1-one (ODQ), indomethacin, compound C (CC), GW501516, mannitol, palmitate acid (PA), d-glucose, dihydroethidium (DHE), A23187 and tunicamycin (TM) were from Sigma-Aldrich (St. Louis, MO, USA). NG-nitro-l-arginine methyl ester (l-NAME), 1400W and GSK0660 were from Tocris Bioscience (Bristol, UK). L-012 was from Wako Pure Chemical Industries (Osaka, Japan). Recombinant human PPARδ protein and anti-phosphorylated IRE1α (Ser724) antibody were obtained from Abcam (Cambridge, MA, USA). Antibodies against adenosine monophosphate-activated protein kinase (AMPK), phospho-AMPK (Thr172), activating transcription factor 4 (ATF4), ATF6, GRP78, protein-disulfide isomerase (PDI), IRE1α and Griess reagent nitrite measurement kit were from Cell Signaling Technology (Danvers, MA, USA). Antibodies against PERK and phospho-PERK (Thr981), 4-Amino-5-methylamino-2′,7′-difluorofluorescein diacetate (DAF-FM DA) were from ThermoFisher Scientific (Waltham, MA, USA). Antibody against β-actin and horseradish peroxidase (HRP)-conjugated secondary antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).
Nie X., Tang W., Zhang Z., Yang C., Qian L., Xie X., Qiang E., Zhao J., Zhao W., Xiao L, & Wang N. (2020). Procyanidin B2 mitigates endothelial endoplasmic reticulum stress through a PPARδ-Dependent mechanism. Redox Biology, 37, 101728.
+ Open protocol
+ Expand
2

Liver Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse livers were homogenized in ice‐cold RIPA buffer containing protease and phosphatase inhibitors (Thermo‐Fisher), and then, diluted in laemmli buffer containing 5% of β‐mercaptoethanol before being heated at 95°C for 5 min. Proteins were resolved by SDS‐PAGE before being transferred to a nitrocellulose membrane. Membranes were probed with primary antibodies targeting the aryl hydrocarbon receptor (AhR) (Cell Signaling Technologies), cleaved caspase‐3 (Cell Signaling Technologies), Ly6G/6C (Thermo‐Fisher), glycoprotein VI (GPVI) (Millipore, Burlington, MA), 5‐hydroxytrytamine receptor 2B (5‐HT2B) (Novus Biologicals, Littleton, CO), indoleamine 2,3‐dioxygenase (IDO2) (Thermo‐Fisher), nuclear factor kappa‐light‐chain‐enhancer of activated B cells (NFkB) p65 subunit (Cell Signaling Technologies), phospho‐NFkB p65 subunit (Cell Signaling Technologies), Poly [ADP‐ribose] polymerase 1 (PARP‐1) (Proteintech, Rosemont, IL), protein disulfide isomerase (PDI, Cell Signaling Technologies), or vinculin (Cell Signaling Technologies) overnight before being probed with an appropriate secondary antibody for 1 hr. The Clarity electrochemiluminescent substrate kit and ChemiDoc MP imaging system (BioRad, Hercules, CA) were used for detection and image acquisition.
Eudy B.J., McDermott C.E., Liu X, & da Silva R.P. (2020). Targeted and untargeted metabolomics provide insight into the consequences of glycine‐N‐methyltransferase deficiency including the novel finding of defective immune function. Physiological Reports, 8(18), e14576.
+ Open protocol
+ Expand
3

Autophagy and Inflammation Modulation in Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), Lipofectamine™ 3000 transfection reagent, TRIzol reagent, LysoTracker Red and the high capacity cDNA reverse transcription kit were purchased from Thermo Fisher Scientific (Waltham, MA, USA). Rapa and 3-MA were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). CQ and BAY-11-7082 were obtained from Selleck (Houston, Texas, USA). Mouse anti-human monoclonal antibodies against PreS2/S, β-actin, and histone 3 were purchased from Abcam (C ambridge, UK). Anti-human monoclonal antibodies against LC3, Beclin-1, SQSTM/P62, NF-κB, p-NF-κB/p65, IκB, p-IκB, Vimentin, E-cadherin and Protein Disulfide Isomerase (PDI) were purchased from Cell Signaling Technology (Boston, MA, USA). The Immobilon Western Chemiluminescent HRP substrate was purchased from EMD Millipore (Billerica, MA, USA). The cell counting kit-8 was purchased from Beyotime (Shanghai, China). Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L), Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L), DyLight 405-labeled goat anti-mouse IgG (H + L) and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) were purchased from ZSGB-BIO (Beijing, China). Propidium Iodide (PI)/RNase staining buffer was purchased from BD Biosciences (Franklin, NJ, USA).
Cheng B., Wang Q., Wei Z., He Y., Li R., Liu G., Zeng S, & Meng Z. (2022). MHBSt167 induced autophagy promote cell proliferation and EMT by activating the immune response in L02 cells. Virology Journal, 19, 110.
+ Open protocol
+ Expand
4

Histological and Immunohistochemical Analysis of Embryonic Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
The embryos were fixed in 4% paraformaldehyde (PFA) in phosphate buffered saline (PBS, pH 7.4) at 4°C for 16 hours, followed by dehydration through a series of concentrations of ethanol and embedding in paraffin wax through mediation steps of xylene. Tissue sections at 6 μm thickness were cut using a microtome.
Tissue sections were dewaxed in xylene and rehydrated through a reverse ethanol concentration series to water. For histological preparation, the sections were stained with hematoxylin and eosin (Junqueira and Carneiro, 2003 ).
For immunohistochemistry, the sections were boiled in a citrate buffer (10 mM Citrate Acid, pH 6.0) for 5 minutes to unmask antigens, equilibrated in PBS (pH 7.4), incubated with primary antibodies recognizing 4-hydroxynonenal (4-HNE; Millipore, Temecula, CA), 3-nitrotyrosine (3-NT, Cell Signaling Technology, Danvers, MA), protein disulfide isomerase (PDI; Cell Signaling Technology), phosphorylated (p-) calnexin (Abcam, Cambridge, MA), Inhibin βA (EMD Biosciences, Gibbstown, NJ), and p-Smad3 (Cell Signaling Technology) (Zhao, 2012 (link)).
Signals were detected using secondary antibodies conjugated with alkaline phosphatase (AP; Santa Cruz Biotechnology, Santa Cruz, CA) and its substrates (nitro-blue tetrazolium and 5-bromo-4-chloro-3’-indolyphosphate). Tissue sections were counterstained with fast red.
, & Zhao Z. (2014). TGFβ and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies. Birth defects research. Part B, Developmental and reproductive toxicology, 101(5), 364-370.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!