All the studies using nasal septal chondrocytes (NSCs) were conducted after written approval (HC13TISI0038) obtained from the Institutional Review Board of the Catholic Medical Center Clinical Research Coordinating Center. Human nasal septum tissue was harvested from five patients who were undergoing septoplasty [21 (link)]. Those tissues were cut into 1 mm3 pieces and they were enzymatically digested using 0.2 % protease solution (Gibco) for 60 min, followed by incubation in 0.3 % collagenase (Sigma-Aldrich) for 12 h at 37 °C. The isolated cells were then seeded in a 75-mm2 cell culture flask (NUNC) and cultivated in low-glucose Dulbecco’s Modified Eagle Medium (Gibco-BRL) with 10 % fetal bovine serum (FBS; Gibco) and antibiotics at 37 °C in 5 % CO2 incubator. The confluent chondrocytes were subcultured following a standard protocol using 0.05 % trypsin/EDTA solution (Gibco).
75 mm2 cell culture flask
Manufactured by Thermo Fisher Scientific
The 75-mm2 cell culture flask is a laboratory equipment designed for the cultivation and growth of cells in vitro. It provides a standardized and controlled environment for cell culture experiments.
Automatically generated - may contain errors
Lab products found in correlation
Protease solution, by Thermo Fisher Scientific (2 mentions)
Collagenase, by Merck Group (2 mentions)
Low glucose dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (2 mentions)
2 protocols using 75 mm2 cell culture flask
1
Isolation and Cultivation of Nasal Septal Chondrocytes
2
Isolation and Culture of Human Nasal Septal Chondrocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the studies using nasal septal chondrocytes (NSCs) were conducted after written approval (HC13TISI0038) obtained from the Institutional Review Board of the Catholic Medical Center Clinical Research Coordinating Center. Human nasal septum tissue was harvested from five patients who were undergoing septoplasty [21] .
Those tissues were cut into 1 mm 3 pieces and they were enzymatically digested using 0.2% protease solution (Gibco) for 60 min, followed by incubation in 0.3% collagenase (Sigma-Aldrich) for 12hr at 37 °C. The isolated cells were then seeded in a 75-mm 2 cell culture flask (NUNC) and cultivated in low-glucose Dulbecco's Modified Eagle Medium (Gibco-BRL) with 10% fetal bovine serum (FBS; Gibco) and antibiotics at 37 °C in 5% CO 2 incubator. The confluent chondrocytes were subcultured following a standard protocol using 0.05% trypsin/EDTA solution (Gibco).
Those tissues were cut into 1 mm 3 pieces and they were enzymatically digested using 0.2% protease solution (Gibco) for 60 min, followed by incubation in 0.3% collagenase (Sigma-Aldrich) for 12hr at 37 °C. The isolated cells were then seeded in a 75-mm 2 cell culture flask (NUNC) and cultivated in low-glucose Dulbecco's Modified Eagle Medium (Gibco-BRL) with 10% fetal bovine serum (FBS; Gibco) and antibiotics at 37 °C in 5% CO 2 incubator. The confluent chondrocytes were subcultured following a standard protocol using 0.05% trypsin/EDTA solution (Gibco).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!