Fibrinogen, FITC-BSA conjugate, uricase (Candida sp.), and all chemicals were purchased from Sigma-Aldrich unless otherwise noted and were used as received. Pierce 660 nm Protein Assay Reagent was purchased from Thermo Fisher Scientific. Goat anti-mouse IgM antibody and goat anti-mouse IgG antibody were purchased from Bethyl Laboratories Inc. TNF-α, IL-4, IL-6, IL-10, CXCL-10, and TGF-β Quantikine ELISA kits were purchased from R&D Systems. Anti–CD40-FITC, anti–CD80–phycoerythrin (PE), anti–CD4-PE, anti–CD25-FITC, and anti–Foxp3-Percep antibodies were purchased from BD Bioscience. PEG polymers with different molecule weights were purchased from BroadPharm. Mouse macrophages RAW 264.7 were purchased from American Type Culture Collection. Mouse dendritic cell line DC 2.4 was received from K. L. Rock (University of Massachusetts Medical Center, Worcester, MA) as a gift.
Anti cd80 phycoerythrin pe
Manufactured by BD
Sourced in Belgium
Anti–CD80–phycoerythrin (PE) is a fluorescent-labeled antibody that binds to the CD80 cell surface protein. CD80 is a co-stimulatory molecule expressed on antigen-presenting cells that plays a role in T-cell activation. The phycoerythrin (PE) fluorescent label allows for the detection and analysis of CD80-expressing cells using flow cytometry or similar techniques.
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Lab products found in correlation
Fibrinogen, by Merck Group (1 mentions)
Anti cd40 fitc, by BD (1 mentions)
Pierce 660 nm protein assay reagent, by Thermo Fisher Scientific (1 mentions)
Anti cd25 fitc, by BD (1 mentions)
Cxcl10, by R&D Systems (1 mentions)
Interleukin 6 (il 6), by R&D Systems (1 mentions)
Anti cd4 pe, by BD (1 mentions)
Il 10, by R&D Systems (1 mentions)
Anti cd83 fitc, by Thermo Fisher Scientific (1 mentions)
Anti cd14 percp, by BD (1 mentions)
Facscan flow cytometer, by BD (1 mentions)
2 protocols using anti cd80 phycoerythrin pe
1
Immune Modulatory Nanoparticle Synthesis
2
Phenotypic Characterization of Dendritic Cells
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For phenotypic characterization of DC, direct immunofluorescence staining was performed using the following fluorochrome-labeled mouse anti-human monoclonal antibodies: anti-CD86-fluorescein isothiocyanate (FITC) (BD Pharmingen, Erembodegem, Belgium), anti-CD80-phycoerythrin (PE) (BD Biosciences, Erembodegem, Belgium), anti-human leukocyte antigen- (HLA-) DR-peridinin chlorophyll (PerCP) (BD Biosciences), anti-CD83-FITC (Life Technologies), anti-dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin- (DC-SIGN-) PE (BD Pharmingen), anti-CD14-PerCP (BD Biosciences), anti-programmed death-ligand 1- (PD-L1-) FITC (BD Pharmingen), anti-CCR7-PE (R&D Systems, Abingdon, UK), and anti-immunoglobulin-like transcript 3- (ILT3-) PE-Cy5 (Immunotech, Marseille, France). Isotype-matched control monoclonal antibodies were used to determine nonspecific background staining. Propidium iodide staining was done for analysis of cell viability. For analytical flow cytometry, at least 104 events were analyzed using a BD FACScan flow cytometer (BD Biosciences). All results were analyzed using FlowJo software (Tree Star, Ashland, USA).
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