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4 protocols using xanthan gum

1

Formulation of Functional Food Hydrocolloids

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Commercially available food-grade HPMC (HT-E15), calcium chloride and potassium citrate were obtained from Shandong Ruitai Co., Ltd. (Shandong, China) while sodium alginate, xanthan gum and gellan gum were supplied by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China).
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2

Hydrocolloid-Based Edible Film Formulation

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Commercially available food-grade HPMC (HT-E5), calcium chloride, and potassium citrate were sourced from Shandong Ruitai Co., Ltd. (Feicheng, China), while xanthan gum and gellan gum were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Analytical reagent sodium alginate was also supplied by Shanghai Macklin Biochemical Co., Ltd. (China). Distilled water was used in the preparation of all solutions used in the experiment.
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3

Characterization of Biopolymer-Based Emulsions

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CaCO3 was obtained from Shuang Teng Industrial Co., Ltd. (Henan, China). Sodium caseinate (Lot#C10185129) and Xanthan gum were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). Soybean Oil was supplied from COFCO Co., Ltd. (Tianjin, China). Nile Blue A and Nile Red were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). The stomach pepsin (Lot#L22M9J56574, the activity was 1:10,000) from porcine gastric mucosa, lipase (Lot#T17N9C75063, BR, the activity was 30,000 U/g) from porcine pancreas and bile salt (Lot#K01N8B47111, cholic acid content was ≥60%) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). All other chemicals were analytical grade purchased from Sinopharm Group Co., Ltd. (Beijing, China).
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4

Characterization of MR Products

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All of the chemicals were of analytical grade, unless otherwise stated. The L-lysine, D-glucose, NaCl, xanthan gum, and gum arabic were purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). The acetonitrile and formic acid of HPLC grade were acquired from Merck (Darmstadt, Germany). A solid-phase extraction cartridge, Cleanert PEP-2 (200 mg/6 mL, Bonna-Agela Technologies Inc., Tianjin, China), was used for the purification of the MR products. The pyrraline (purity >99.99%) and 3-deoxyglucosone (purity >99.99%) were supplied by Toronto Research Chemicals (North York, Ontario, Canada). The starches were obtained from Ingredion Incorporated (Shanghai, China).
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