Animals were euthanized and fixed by intracardiac perfusion with a 4% paraformaldehyde solution. Tongues were excised and placed in 30% sucrose solution overnight at 4°C for cryoprotection. Tissues were embedded in OCT compound and sectioned at 30 μm thickness on a cryostat. Sections were washed in PBS with 0.1% Triton X-100 (PBST), blocked with 10% donkey serum in PBST, incubated with primary antibodies overnight at 4°C, and incubated with fluorescence-tagged secondary antibodies (Jackson ImmunoResearch) for 2 hours at room temperature. Primary antibodies used were: anti-Sema7A (R&D Systems #AF1835; 1:300 dilution), anti-FLAG (Sigma #F7425; 1:1000 dilution), anti-T1R3 (Santa Cruz Biotechnology #sc-22458; 1:500 dilution), anti-Car4 (R&D Systems #AF2414; 1:500 dilution), anti-Plcβ2 (Santa Cruz Biotechnology #sc-206; 1:1000 dilution), anti-GFP (Abcam #ab13970; 1:500 dilution), anti-Nrp1 (R&D Systems #AF566; 1:200 dilution), and anti-PlxnC1 (R&D Systems #AF5375; 1:200 dilution).
Af2414
Manufactured by Santa Cruz Biotechnology
AF2414 is a laboratory instrument used for cell separation and purification. It utilizes advanced technology to isolate and enrich target cells from complex biological samples. The core function of AF2414 is to enable efficient cell separation and purification for various applications in the life sciences and biomedical research fields.
Automatically generated - may contain errors
Lab products found in correlation
Ab13970, by R&D Systems (2 mentions)
Af566, by R&D Systems (2 mentions)
Fluorescence tagged secondary antibodies, by Jackson ImmunoResearch (2 mentions)
Af1835, by Merck Group (2 mentions)
Sc 22458, by R&D Systems (2 mentions)
Sc 206, by Abcam (2 mentions)
Af5375, by R&D Systems (2 mentions)
F7425, by Merck Group (2 mentions)
2 protocols using af2414
1
Immunofluorescence Characterization of Taste Receptors
2
Immunofluorescence Characterization of Taste Receptors
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Animals were euthanized and fixed by intracardiac perfusion with a 4% paraformaldehyde solution. Tongues were excised and placed in 30% sucrose solution overnight at 4°C for cryoprotection. Tissues were embedded in OCT compound and sectioned at 30 μm thickness on a cryostat. Sections were washed in PBS with 0.1% Triton X-100 (PBST), blocked with 10% donkey serum in PBST, incubated with primary antibodies overnight at 4°C, and incubated with fluorescence-tagged secondary antibodies (Jackson ImmunoResearch) for 2 hours at room temperature. Primary antibodies used were: anti-Sema7A (R&D Systems #AF1835; 1:300 dilution), anti-FLAG (Sigma #F7425; 1:1000 dilution), anti-T1R3 (Santa Cruz Biotechnology #sc-22458; 1:500 dilution), anti-Car4 (R&D Systems #AF2414; 1:500 dilution), anti-Plcβ2 (Santa Cruz Biotechnology #sc-206; 1:1000 dilution), anti-GFP (Abcam #ab13970; 1:500 dilution), anti-Nrp1 (R&D Systems #AF566; 1:200 dilution), and anti-PlxnC1 (R&D Systems #AF5375; 1:200 dilution).
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