Ki 67 clone mib 1

Manufactured by Abcam

Sourced in United States

Ki-67 (clone MIB-1) is a monoclonal antibody that binds to the Ki-67 protein, a cellular marker for proliferation. This antibody is used in immunohistochemistry and flow cytometry applications to detect and quantify proliferating cells.

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Lab products found in correlation

Hla dr clone ln3, by Abcam (1 mentions) Cd3 clone ln10, by Leica camera (1 mentions) Cd8 clone 4b11, by Leica camera (1 mentions) Sox10 clone bc34, by Biocare Medical (1 mentions) Opal multiplex 6 plex kits, by PerkinElmer (1 mentions)

2 protocols using ki 67 clone mib 1

Quantitative Ki-67 Immunohistochemistry

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The primary antibody, Ki-67 (clone MIB-1; dilution of 1:100) (Abcam, Cambridge, MA, USA), and a universal secondary antibody (dilution of 1:300) (Roche, Basel, Swizerland) were used. The cell proliferation index was calculated by taking five random pictures of each slide at a magnification of 200 × (10 ocular × 20 objective). The index was calculated as the number of Ki-67-stained cells divided by the total number of cells. HematoxylinIIwas used for the counterstaining for cell nuclei.

Tsuji Y., Nonoguchi N., Okuzaki D., Wada Y., Motooka D., Hirota Y., Toho T., Yoshikawa N., Furuse M., Kawabata S., Miyatake S.I., Nakamura H., Yamamoto R., Nakamura S., Kuroiwa T, & Wanibuchi M. (2021). Chronic pathophysiological changes in the normal brain parenchyma caused by radiotherapy accelerate glioma progression. Scientific Reports, 11, 22110.

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Multiplex Immunofluorescence Staining of Tissue Sections

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Full section 5 μm slides of tissue specimens were stained using Opal™ multiplex 6-plex kits, according to the manufacturer's protocol (PerkinElmer), for DAPI, CD3 (clone LN10; Leica (Buffalo Grove, IL); 1:200 dilution), CD8 (clone 4B11; Leica; Ready to use (RTU)), CD68 (clone KP1; Biogenex (Fremont, CA), RTU); SOX10 (clone BC34; Biocare (Pacheco, CA); 1:300), HLA-DR (clone LN-3; Abcam (Cambridge, MA); 1:200 dilution), and Ki67 (clone MIB1; Abcam; RTU). Opal multiplexing is a serial immunohistochemistry method that relies on tyramide signal amplification (TSA)(30 (link), 45 (link)), which creates an amplification of signal that then covalently binds to the epitope in a specific manner (27 (link), 46 (link)). Primary and secondary antibody complexes are subsequently removed for serial immunofluorescence, while the covalent fluorescent signal remains. Single controls and an unstained slide were stained with each group of slides.

Gartrell R.D., Marks D.K., Hart T.D., Li G., Davari D.R., Wu A., Blake Z., Lu Y., Askin K.N., Monod A., Esancy C.L., Stack E.C., Jia D.T., Armenta P.M., Fu Y., Izaki D., Taback B., Rabadan R., Kaufman H.L., Drake C.G., Horst B.A, & Saenger Y.M. (2018). Quantitative Analysis of Immune Infiltrates in Primary Melanoma. Cancer immunology research, 6(4), 481-493.

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