Body weight (kg), height (m) and waist circumference (cm) were measured in the standing position. Hcy levels were measured via fluorescence detection (F-1080, Hitachi Ltd., Tokyo, Japan) on high-performance liquid chromatography (HPLC; LC-9A, Shimadzu Corp., Kyoto, Japan). Glu was measured using the hexokinase method. TC, HDL-C, TG, alanine aminotransferase (ALT), aspartate transaminase (AST), and hemoglobin (Hb) were using an autoanalyzer (Cobas c 501 autoanalyzer, Roche Diagnostics, Germany).
F 1080
Manufactured by Hitachi
Sourced in Japan
The F-1080 is a high-performance laboratory equipment designed for scientific research and analysis. Its core function is to provide precise measurements and data analysis capabilities for various types of samples and materials. The F-1080 is engineered to deliver accurate and reliable results, enabling researchers and scientists to conduct their work efficiently and effectively.
Automatically generated - may contain errors
4 protocols using f 1080
1
Anthropometric Measurements and Biomarker Analysis
2
Genotyping MTHFR Polymorphisms and Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MTHFR polymorphisms were detected by using a gene chip hybrid analysis. Venous blood samples were collected in ethylenediaminetetraacetic acid (EDTA) tubes. DNA was extracted from whole blood using the QIAampR DNA Mini Kit (CAT No. 51304, Germany). The MTHFR genotype was determined by PCR-genotyping microarray analysis of three gene types (MTHFR Genotyping kit, BaiO, Shanghai, China). The detection of MTHFR mutation was performed in accordance with the manuals of the Gene Array Kit (BaiO Genotype Detection Gene Array Kit, Cat. No. 51304, Germany) and equipment (BaiO Technology Corp., Shanghai, China). Homocysteine (Hcy) levels were determined by fluorescence detection (F-1080, Hitachi Ltd., Tokyo, Japan) on high-performance liquid chromatography (HPLC; LC-9A, Shimadzu Corp., Kyoto, Japan). Fasting plasma glucose (Glu) was detected by using the hexokinase method. Last, the total cholesterol (TC), high-density lipoprotein (HDL), triglyceride (TG), hemoglobin (Hb), alanine aminotransferase (ALT), and aspartate transaminase (AST) were measured by an autoanalyzer (Cobas c 501 autoanalyzer, Roche Diagnostics, Germany).
3
Plasma Homocysteine Determination Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood samples were collected in the morning after fasting for over 8 h. All samples were centrifuged within 2 h, and serums were stored at −80°C. Plasma Hcy was analyzed by HPLC (LC-9A, Shimadzu Corp., Kyoto, Japan) with fluorescence detection (F-1080, Hitachi Ltd., Tokyo, Japan). Serum Hcy ≥15 μmol/L was diagnosed as Hhcy (21 (link)).
4
Comprehensive Metabolic Profiling of Participants
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The patients were subjected to a health examination, including height and weight measurements and blood pressure. Blood was drawn from fasting subjects, and tubes with plasma containing EDTA were stored at –80℃ for biochemical analyses. Plasma Hcy was analyzed by HPLC (LC‐9A,Shimadzu Corp., Kyoto, Japan) with fluorescence detection (F‐1080, Hitachi Ltd., Tokyo, Japan) 24 . Serum folate concentration was measured using a dual count Solid Phase Boil Radioassay (Diagnostic Products, Los Angeles, CA). Serum Vit B12 was detected by liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS, Agilent Model 6410) 25 . The blood glucose levels, including FPG(fasting plasma glucose) and G2h (postprandial 2 hours blood glucose) were measured using the hexokinase method; the total cholesterol (TC), high‐density lipoprotein (HDL), low‐density lipoprotein (LDH), triglyceride (TG), hemoglobin (HGB), blood uric acid (BUA), alanine aminotransferase (ALT), aspartate transaminase (AST), and blood creatinine (Cr) were measured with an autoanalyzer (Cobas c 501 autoanalyzer, Roche Diagnostics, Germany). C‐reactive protein (CRP) was measured using commercially available enzyme‐linked immunosorbent assay kits (ELISA kits, Biosource, Int. CA). Standard quality control procedures were performed each day with standard samples (CV < 10%).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!