Female C57BL/6 mice aged at 6–8 weeks were purchased from Jinan Pengyue animal company. All animals received humane care following the Guide for the Care and Use of Laboratory Animals approved by Shandong University. Throughout the study period, all animals had access to food and water ad libitum and were maintained on a 12 h light/dark cycle (21 ± 2°C with a relative humidity of 45 ± 5%). After one week of adaptation, animals were randomly divided into the following four groups: normal diet (C group), sham-operated+ high fat diet (HFD, 60 kcal% Fat, research diets, New Brunswick, Canada) (SH group), OVX+HFD (OH group), OVX+HFD+FMT (OHF group). Two weeks after OVX, the mice were given HFD. FMT was performed based on an established protocol [16 ]. Control animals received the same volume of saline solution. After HFD administration for 4 weeks, mice were anesthetized using 2% Isoflurane followed by cardiac puncture and the blood was collected. Whole livers were immediately prepared for histological analysis; some were stored at -80°C for RNA and western blot preparation, and blood samples were collected for biochemical analysis. Liver function was assayed by the serological activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by using the Hitachi 7600–020 clinical analyzer (Hitachi, Tokyo, Japan).
7600 020 clinical analyzer
Manufactured by Hitachi
Sourced in Japan
The Hitachi 7600-020 clinical analyzer is a fully automated, high-throughput instrument designed for clinical chemistry analysis. It is capable of performing a wide range of routine and specialized diagnostic tests on a variety of sample types.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using 7600 020 clinical analyzer
1
Ovariectomy, High-Fat Diet, and Gut Microbiota
2
Effect of FMT on Obesity-Induced NAFLD
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female C57BL/6 mice aged at 6-8 weeks were purchased from Jinan Pengyue animal company. All animals received humane care following the Guide for the Care and Use of Laboratory Animals approved by Shandong University. Throughout the study period, all animals had access to food and water ad libitum and were maintained on a 12 h light/dark cycle (21 ± 2℃ with a relative humidity of 45 ± 5%).
After one week of adaptation, animals were randomly divided into the following four groups (n=8):
normal diet (C group), sham-operated+ high fat diet (HFD, 60 kcal% Fat, research diets, New Brunswick, Canada) (SH group), OVX+HFD (OH group), OVX+HFD+FMT (OHF group). Two weeks after OVX, the mice were given HFD. FMT was performed based on an established protocol [17] . Control animals received the same volume of saline solution. Animals were sacri ced 4 w after HFD administration; whole livers were immediately prepared for histological analysis; some materials were stored at -80°C for RNA and western blot preparation, and blood samples were collected for biochemical analysis. Liver function was assayed by the serological activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by using the Hitachi 7600-020 clinical analyzer (Hitachi, Tokyo, Japan).
After one week of adaptation, animals were randomly divided into the following four groups (n=8):
normal diet (C group), sham-operated+ high fat diet (HFD, 60 kcal% Fat, research diets, New Brunswick, Canada) (SH group), OVX+HFD (OH group), OVX+HFD+FMT (OHF group). Two weeks after OVX, the mice were given HFD. FMT was performed based on an established protocol [17] . Control animals received the same volume of saline solution. Animals were sacri ced 4 w after HFD administration; whole livers were immediately prepared for histological analysis; some materials were stored at -80°C for RNA and western blot preparation, and blood samples were collected for biochemical analysis. Liver function was assayed by the serological activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) by using the Hitachi 7600-020 clinical analyzer (Hitachi, Tokyo, Japan).
3
Histological and Biochemical Liver Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 12 weeks of STZ + SITA, mice were killed by injecting pentobarbital (150 mg/kg body weight) at the termination of the experiments. Liver tissues were fixed in 4% paraformaldehyde and embedded in paraffin for hematoxylin and eosin staining and Sirius red staining.
For a-SMA and F4/80 staining, anti-a-SMA (ab124964, Abcam, Cambridge, UK) and anti-F4/80 (28463-1-AP, Proteintech, Chicago, USA) primary antibodies at 1 : 300 dissolved in 3% bovine serum albumin (BSA)–phosphate-buffered saline at 4 °C overnight. Quantitative results were calculated by Image-Pro Plus software to average the percentage of positive area for five images in each section.
Serum samples were collected for the assay of ALT, AST, and LDH levels with Hitachi 7600-020 clinical analyzer (Tokyo, Japan).
For a-SMA and F4/80 staining, anti-a-SMA (ab124964, Abcam, Cambridge, UK) and anti-F4/80 (28463-1-AP, Proteintech, Chicago, USA) primary antibodies at 1 : 300 dissolved in 3% bovine serum albumin (BSA)–phosphate-buffered saline at 4 °C overnight. Quantitative results were calculated by Image-Pro Plus software to average the percentage of positive area for five images in each section.
Serum samples were collected for the assay of ALT, AST, and LDH levels with Hitachi 7600-020 clinical analyzer (Tokyo, Japan).
4
Metabolic Profiling of Diet-Induced Liver Disorders
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After 4 weeks of MCD or CDE diet, or 16 weeks of HFD, mice were sacrificed at termination of the experiments. The livers were collected for histological analysis, and blood samples were collected for the assay of alanine aminotransferase (ALT), aspartate aminotransferase (AST), triglyceride (TG), cholesterol (TC), high‐density lipoprotein cholesterol (HDL‐C), and low‐density lipoprotein cholesterol (LDL‐C) with an Hitachi 7600‐020 clinical analyzer (Tokyo, Japan). Briefly, the liver tissues (50 mg) were homogenized for measurement of tissue lipid content of TG/TC and HDL‐C/LDL‐C as well using commercial kits (for TG/TC, Applygen Technologies [Beijing, China]; for HDL‐C/LDL‐C, Jiancheng Bioengineering Institute [Nanjing, China]).
5
Hepatic Lipid Content Analysis in Lrp6 Mutant Mice
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!