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Ht 29 luc2

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The HT-29-luc2 is a human colorectal adenocarcinoma cell line that has been genetically modified to express the luciferase reporter gene. It is commonly used in cellular assays and research applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Ht 29 luc2, by Thermo Fisher Scientific (4 mentions) Sw480, by Thermo Fisher Scientific (3 mentions) Ht 29, by Thermo Fisher Scientific (3 mentions) Dmem f12, by Thermo Fisher Scientific (3 mentions) Caco 2, by Thermo Fisher Scientific (2 mentions) Rpmi 1640, by Thermo Fisher Scientific (2 mentions) Nci h1373, by American Type Culture Collection (1 mentions) Nci h1838, by American Type Culture Collection (1 mentions) Du4475, by American Type Culture Collection (1 mentions) A2780, by American Type Culture Collection (1 mentions) Cal27, by American Type Culture Collection (1 mentions) E myco pcr kit, by iNtRON Biotechnology (1 mentions) Nutrient mixture f 12 dmem f 12, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)

7 protocols using ht 29 luc2

1

Authenticated HT29 Cell Culture

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Luciferase-tagged HT29 cells (HT-29-luc2 from Caliper Life Sciences) were authenticated by STR typing and regularly tested for mycoplasma and shown to be contamination free. They were cultured in DMEM supplemented with 10% FCS, L-glutamine and penicillin/streptomycin at 37°C in an atmosphere of 5% CO2.
Frentzas S., Simoneau E., Bridgeman V.L., Vermeulen P.B., Foo S., Kostaras E., Nathan M., Wotherspoon A., Gao Z.H., Shi Y., Van den Eynden G., Daley F., Peckitt C., Tan X., Salman A., Lazaris A., Gazinska P., Berg T.J., Eltahir Z., Ritsma L., Van Rheenen J., Khashper A., Brown G., Nystrom H., Sund M., Van Laere S., Loyer E., Dirix L., Cunningham D., Metrakos P, & Reynolds A.R. (2016). Vessel co-option mediates resistance to anti-angiogenic therapy in liver metastases. Nature medicine, 22(11), 1294-1302.
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2

Cell Line Provenance and Authentication

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CAL27, FaDu, A549 (obtained 2013), PJ34, PJ41 (obtained 2014), T24, A2780, RT4 (obtained 2012), NCI-H1838 (2013), NCI-H1373 (2015), DU-4475 (year unknown, STR profiled 2016) cell lines were purchased from ATCC. SCC090, and SCC131 were purchased from DSMZ (2014). SK-OV-3 (2011) was obtained from HPA culture collection (Porton Down). HCT116 p53 Xman isogenic cell lines were purchased from Horizon Discovery (2014), DLD1 BRCA2 wild-type and deficient cells were provided to the ICR as part of a collaborative agreement. HT-29-luc2 was purchased from Caliper (2015). LON-LICR-HN4 and LON-LICR-HN5 cell lines from Professor Sue Eccles (ICR, London, 2012). STR profiling was carried out by Bio-Synthesis Inc. Mycoplasma testing used the e-Myco PCR kit (Intron Biotechnology). Cell lines received from noncommercial sources were STR profiled prior to freezing, all cell lines were mycoplasma tested, and experiments were carried out within 3 months of resuscitation.
Dillon M.T., Barker H.E., Pedersen M., Hafsi H., Bhide S.A., Newbold K.L., Nutting C.M., McLaughlin M, & Harrington K.J. (2016). Radiosensitization by the ATR Inhibitor AZD6738 through Generation of Acentric Micronuclei. Molecular cancer therapeutics, 16(1), 25-34.
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3

Colorectal Cancer Cell Lines for Research

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Human colorectal cancer cell lines (HT-29, Caco2 and SW480) were acquired from the Tissue Culture Facility at UNC Lineberger Comprehensive Cancer Center. The luciferase-expressing cell line, HT-29-luc2, was purchased from Caliper Life Sciences (Hopkinton, MA). Another luciferase-expressing cell line, CRC119, was derived from human colorectal cancer liver metastases and obtained from Dr. David Hsu from Duke University. Cell lines were authenticated using short tandem repeat and were tested for mycoplasma contamination. HT-29, HT-29-luc2, and SW480 cells were cultured in DMEM/F12 (Gibco) supplemented with 10% fetal bovine serum (Gibco) and penicillin/streptomycin (Mediatech). Caco2 cells were cultured in DMEM/F12 (Gibco) supplemented with 20% fetal bovine serum (Gibco) and penicillin/streptomycin (Mediatech). CRC119 cells were cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco) and penicillin/streptomycin (Mediatech). Cells were passaged on normal tissue culture plates or tissue culture plates coated with collagen, Matrigel, liver BMSs, and lung BMSs (100 ug/cm2).
Tian X., Werner M.E., Roche K.C., Hanson A.D., Foote H.P., Yu S.K., Warner S.B., Copp J.A., Lara H., Wauthier E.L., Caster J.M., Herring L.E., Zhang L., Tepper J.E., Hsu D.S., Zhang T., Reid L.M, & Wang A.Z. (2018). Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds. Nature biomedical engineering, 2(6), 443-452.
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4

Authenticated HT29 Cell Culture

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Luciferase-tagged HT29 cells (HT-29-luc2 from Caliper Life Sciences) were authenticated by STR typing and regularly tested for mycoplasma and shown to be contamination free. They were cultured in DMEM supplemented with 10% FCS, L-glutamine and penicillin/streptomycin at 37°C in an atmosphere of 5% CO2.
Frentzas S., Simoneau E., Bridgeman V.L., Vermeulen P.B., Foo S., Kostaras E., Nathan M., Wotherspoon A., Gao Z.H., Shi Y., Van den Eynden G., Daley F., Peckitt C., Tan X., Salman A., Lazaris A., Gazinska P., Berg T.J., Eltahir Z., Ritsma L., Van Rheenen J., Khashper A., Brown G., Nystrom H., Sund M., Van Laere S., Loyer E., Dirix L., Cunningham D., Metrakos P, & Reynolds A.R. (2016). Vessel co-option mediates resistance to anti-angiogenic therapy in liver metastases. Nature medicine, 22(11), 1294-1302.
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5

Culturing Colorectal Cancer Cell Lines

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Human colorectal cancer cell lines HT-29 (ATCC batch F-9246) and SW480 cells (ATCC batch 7265) were obtained from the Tissue Culture Facility at the LCCC at UNC in 2014. Cell line authentication was performed via short tandem repeat in 2014. The luciferase-expressing cell line HT-29-luc2 was purchased from Caliper Life Sciences (Hopkinton, MA) in 2014. All cell lines were used within 10 passages after initial plating. HT-29, SW480, and HT-29-luc2 cells were cultured at 37°C in 5% CO2 humidified atmosphere in Dulbecco’s Modified Eagle Medium: Nutrient Mixture F-12 (DMEM/F12) (Gibco) supplemented with 10% (vol/vol) fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Gibco).
Tian X., Nguyen M., Foote H.P., Caster J.M., Roche K.C., Peters C.G., Wu P., Jayaraman L., Garmey E.G., Tepper J.E., Eliasof S, & Wang A.Z. (2016). CRLX101, a nanoparticle-drug conjugate containing camptothecin, improves rectal cancer chemoradiotherapy by inhibiting DNA repair and HIF-1α. Cancer research, 77(1), 112-122.
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6

Cell Line Maintenance and Characterization

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HT-29-luc2, was purchased from Caliper Life Sciences (Hopkinton, MA) and murine CRC CT26-Luc cells were obtained from Tissue Culture Facility—UNC Lineberger Comprehensive Cancer Center. Cell lines used were authenticated by the vendors and tested for mycoplasma contamination by source. HT-29-luc2 cells were cultured in DMEM/F12 (Gibco, Gaithersburg, MD) supplemented with 10% fetal bovine serum (Gibco, MD) and penicillin/streptomycin (Mediatech, Manassas, VA). CT26-Luc cells were cultured by DMEM (Gibco, MD), supplemented with 10% fetal bovine serum (Gibco, MD), penicillin/streptomycin (Mediatech, VA) and 0.4 mg/mL G418 (Gibco, MD).
Sendi H., Yazdimamaghani M., Hu M., Sultanpuram N., Wang J., Moody A.S., McCabe E., Zhang J., Graboski A., Li L., Rojas J.D., Dayton P.A., Huang L, & Wang A.Z. (2021). Nanoparticle delivery of miR-122 inhibits colorectal cancer liver metastasis. Cancer research, 82(1), 105-113.
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7

Colorectal Cancer Cell Lines for Research

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Human colorectal cancer cell lines (HT-29, Caco2 and SW480) were acquired from the Tissue Culture Facility at UNC Lineberger Comprehensive Cancer Center. The luciferase-expressing cell line, HT-29-luc2, was purchased from Caliper Life Sciences (Hopkinton, MA). Another luciferase-expressing cell line, CRC119, was derived from human colorectal cancer liver metastases and obtained from Dr. David Hsu from Duke University. Cell lines were authenticated using short tandem repeat and were tested for mycoplasma contamination. HT-29, HT-29-luc2, and SW480 cells were cultured in DMEM/F12 (Gibco) supplemented with 10% fetal bovine serum (Gibco) and penicillin/streptomycin (Mediatech). Caco2 cells were cultured in DMEM/F12 (Gibco) supplemented with 20% fetal bovine serum (Gibco) and penicillin/streptomycin (Mediatech). CRC119 cells were cultured in RPMI 1640 (Gibco) supplemented with 10% fetal bovine serum (FBS) (Gibco) and penicillin/streptomycin (Mediatech). Cells were passaged on normal tissue culture plates or tissue culture plates coated with collagen, Matrigel, liver BMSs, and lung BMSs (100 ug/cm2).
Tian X., Werner M.E., Roche K.C., Hanson A.D., Foote H.P., Yu S.K., Warner S.B., Copp J.A., Lara H., Wauthier E.L., Caster J.M., Herring L.E., Zhang L., Tepper J.E., Hsu D.S., Zhang T., Reid L.M, & Wang A.Z. (2018). Organ-specific metastases obtained by culturing colorectal cancer cells on tissue-specific decellularized scaffolds. Nature biomedical engineering, 2(6), 443-452.
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