Pure APIs were used to prepare 6 dry mixtures. The mass ratio of TAZ:PIP ranged from 50:50 to 5:95. Using an analytical balance (Kern Inc., Grove City, OH, USA, ABJ 220-4NM) and depending on the desired ratio, an appropriate amount of each API was obtained in order for a total mass of 100 mg of the standard mixture to be prepared (e.g., for the dry mixture 25:75, 25 mg TAZ and 75 mg PIP were obtained). Each mixture was placed in a special plastic container (NALGENE®, Rochester, NY, USA) having a magnetic rod and was homogenized by placing the latter on a magnetic stirrer (HANNA instruments, HI 190M (Woonsocket, RI, USA)) for 5 min.
Hi 190m
Manufactured by Hanna Instruments
Sourced in United States
The HI 190M is a multi-parameter laboratory meter designed for measuring pH, ORP, and temperature. It features a large LCD display and intuitive user interface. The meter supports automatic calibration and temperature compensation, providing precise and reliable measurements.
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Lab products found in correlation
4 protocols using hi 190m
1
Dry Mixture Preparation of API
2
Surfactant-Stabilized PTFE Ink Preparation
3
Preparation of Dry API Mixtures
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Pure APIs were used to prepare five dry mixtures. The mass ratio of TAZ:PIP ranged from 30:70 to 10:90. Using an analytical balance (Kern Inc., Grove City, OH, USA, ABJ 220-4NM) and depending on the desired ratio, an appropriate amount of each API was obtained in order a total mass of 100 mg of the standard mixture to be prepared (e.g., for the dry mixture 20:80, 20 mg TAZ and 80 mg PIP were obtained). Each mixture was placed in a special plastic container (NALGENE®, Rochester, NY, USA) with a magnetic rod and was homogenized by placing the latter on a magnetic stirrer (HANNA instruments, HI 190M (Woonsocket, RI, USA)) for 5 min.
4
Liposome Preparation with Surface-Active Agents
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MLV, LUV and SUV liposomes with incorporated SA were prepared according to the thin-film hydration method, as previously described (Milenkovic et al., 2013 (link), Petrović et al., 2014 ) with slight modifications. The PC lipid was dissolved in chloroform at the molar concentration of 5 × 10−4 M. The SA dissolved in PBS was added to the lipid at the concentration of 1 × 10–5 M, considering the final buffer volume of liposome suspension. The obtained liposome dispersions after preparation procedure were MLVs. In order to obtain LUVs and SUVs, the liposome dispersion was vortexed (HI 190M, Hanna instruments, Italy) at a speed of 500 rpm, and then extruded (LiposoFast-Basic Extruder, Avestin, Inc. Canada) through poly-carbon filters of 400 and 100 nm pore size respectively. UV–Vis absorption spectroscopy was used to rapidly estimate the dimension and lamellarity of liposomes with incorporated SA. All the operations have been performed above the critical temperature (Tc) of lipid in order to avoid defects. After preparation, all dispersions were stored at 4 °C and after 24 h their characterization was performed. The spectral data have been processed by using the software Origin 8.0.
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