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Ultra 2 directional rna library preparation kit

Manufactured by New England Biolabs
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The Ultra II directional RNA library preparation kit is a tool used in molecular biology laboratories to prepare RNA samples for sequencing. It is designed to generate directional RNA libraries, which preserve the original orientation of the RNA molecules. The kit includes reagents and protocols necessary for RNA fragmentation, adapter ligation, and cDNA synthesis.

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Lab products found in correlation

Nebnext poly a mrna magnetic isolation module, by New England Biolabs (1 mentions) Nextseq, by Illumina (1 mentions)

Close spelling variants of this product

NEBNext Ultra II Directional RNA library preparation kit (15 citations) RNA Ultra Directional kit (7 citations) Ultra II Directional RNA Library kit (4 citations) Ultra directional RNA library kit (3 citations) Ultra II (3 citations)

2 protocols using ultra 2 directional rna library preparation kit

1

Methylation-Based RNA Sequencing

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MeRIP sequencing (MeRIP-seq) libraries were prepared using the Ultra II directional RNA library preparation kit (NEB) as per manufacturer’s instructions for already fragmented samples. As input 5–50 ng RNA was used. Libraries were prepared for MeRIP input and elution samples. RNA-sequencing libraries were prepared from 500 ng total RNA using the Ultra II directional RNA library preparation kit (NEB) in combination with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Single end sequencing of 76 bp was performed on NextSeq (Illumina).
Artz O., Ackermann A., Taylor L., Koo P.K, & Pedmale U.V. (2023). Light and temperature regulate m6A-RNA modification to regulate growth in plants. bioRxiv.
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2

Diverse Library Preparation Protocols

Cited 1 time
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Library preparations for PacBio (Lewis et al. 2016 (link)), Hi-C (Lewis et al. 2019 (link)), and ATAC-seq (Lewis and Reed 2018 ) libraries were performed as described previously. RNA-seq libraries were prepared using the NEB Ultra II Directional RNA library preparation kit. PRO-seq libraries were performed following a low-cost protocol with minor modifications for insect cells (Chu et al. 2018 (link)). Libraries were sequenced at either 2 × 150 bp, 1 × 75 bp (PRO-seq), or 2 × 37 bp (ATAC-seq) on a HiSeq400 or NextSeq500 (ATAC-seq and PRO-seq).
Lewis J.J., Cicconardi F., Martin S.H., Reed R.D., Danko C.G, & Montgomery S.H. (2021). The Dryas iulia Genome Supports Multiple Gains of a W Chromosome from a B Chromosome in Butterflies. Genome Biology and Evolution, 13(7), evab128.
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