The antibodies used in this study were anti-Myc antibody (sc-40, Santa Cruz), anti-HA antibody (sc-57592, Santa Cruz), anti-HBO1 antibody (sc-13284, Santa Cruz), anti-DDB2 antibody (sc-81246, Santa Cruz), anti-XPC antibody (sc-30156, Santa Cruz), anti-TFIIH p89 antibody (sc-293, Santa Cruz), anti-CPD antibody (NMDND001, Cosmo Bio), anti-Orc2 antibody (sc-13238, Santa Cruz), anti-acetyl-Histone H4 antibody (#06-866 Millipore), anti-Histone H4 antibody (ab10158, Abcam), anti-acetyl-Histone H3K14 antibody (A-4023, EPIGENTEK), anti-Histone H3 (tri methyl K4) antibody (ab1012, Abcam), anti-Histone H3 antibody (39763, ACTIVE MOTIF), anti-γH2AX antibody (50-171-736, Upstate), anti-SNF2H antibody (ab3749, Abcam), anti-ACF1 antibody (NB100-61041, Novusbio), anti-CSB antibody (553C5a, BIO MATRIX RESEARCH), anti-UVsS-A antibody (H00057654-B01, Abnova) and anti-β-actin antibody (sc-47778, Santa Cruz). Phospho Ser50 and Ser53 HBO1 rabbit polyclonal antibodies were generated by immunization with a synthetic phosphopeptide (CSARLpSQSpSQD). To perform immunoprecipitation of GFP-SNF2H, anti-GFP mAb-Agarose (D153-8, MBL) was used.
Anti cpd antibody
Manufactured by Cosmo Bio
Sourced in Japan
The Anti-CPD antibody is a laboratory reagent used in research applications. It is designed to detect and bind to cyclobutane pyrimidine dimers (CPDs), which are a type of DNA damage caused by ultraviolet radiation exposure. The antibody can be utilized in various analysis techniques, such as immunoassays and immunohistochemistry, to study DNA repair mechanisms and the effects of UV exposure on cellular processes.
Automatically generated - may contain errors
Lab products found in correlation
Ab1012, by Abcam (1 mentions)
Ab3749, by Abcam (1 mentions)
Ab10158, by Abcam (1 mentions)
Anti myc antibody sc 40, by Santa Cruz Biotechnology (1 mentions)
Anti xpc antibody, by Santa Cruz Biotechnology (1 mentions)
Sc 47778, by Santa Cruz Biotechnology (1 mentions)
Anti acetyl histone h4 antibody, by Merck Group (1 mentions)
Dapi containing mounting medium, by Vector Laboratories (1 mentions)
2 protocols using anti cpd antibody
1
Antibody Characterization for Protein Study
2
Quantifying Cyclobutane Pyrimidine Dimers in UVB-Exposed Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The amount of CPD was determined by ELISA and immunofluorescence microscopy, as described previously [42 (link)]. For ELISA, genomic DNA was reacted with anti-CPD antibody (Cosmo Bio, Tokyo, Japan). For immunofluorescence microscopy, cells were seeded onto 4-well chamber slides at 0.35 × 105 cells per cm2 (0.6 × 105 cells/well). The cells were treated with horse oil (0.312%), and after 1 h exposure to UVB, the cells were incubated at 37 °C for an additional 1 h. After fixation, permeabilization, and denaturation of the DNA, the cells were blocked with FBS, exposed to anti-CPD antibody and Alexa Fluor 594-conjugated F(ab′)2 fragment of anti-mouse IgG, and transferred to microscope slides in DAPI containing mounting medium (Vector Laboratories, Burlingame, CA, USA). Images were collected on a Zeiss confocal microscope using the LSM 510 program.
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