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Glutathione (gsh)

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Lab products found in correlation

Lc ms grade formic acid, by Thermo Fisher Scientific (2 mentions) N acetyl l cysteine, by Roche (2 mentions) Deoxyribonuclease 2, by Merck Group (2 mentions) N acetyl cysteine (nac), by Cambridge Isotopes (2 mentions) Ribonuclease t1, by Merck Group (2 mentions) L cysteine cys, by Merck Group (2 mentions) Acetonitrile hplc ms grade, by Avantor (2 mentions) Phosphodiesterase 2, by Merck Group (2 mentions) Phosphodiesterase 1, by Worthington (2 mentions) Nuclease p1, by Merck Group (2 mentions) Ribonuclease a, by Merck Group (2 mentions) Ammonium formate, by Merck Group (2 mentions) Hplc grade methanol, by Merck Group (2 mentions) Alkaline phosphatase, by Merck Group (2 mentions)

3 protocols using glutathione (gsh)

1

RBC Glutathione Quantification by LC-MS

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RBC-GSH concentration was measured by an exogenous isotope dilution method using [Gly-13C2, 15N]-GSH (Cambridge Isotope Laboratories) as an internal standard. Briefly, 100 μL of the baseline RBC-free sample was spiked with a known quantity of isotopically labeled [Gly-13C2, 15N]-GSH. The samples were then analyzed by liquid chromatography mass spectrometry as described above. The spiked RBC-GSH was analyzed by selected reaction monitoring at precursor ions m/z 308 and 311 to product ions m/z 162 and 165, respectively.
Lutchmansingh F.K., Hsu J.W., Bennett F.I., Badaloo A.V., McFarlane-Anderson N., Gordon-Strachan G.M., Wright-Pascoe R.A., Jahoor F, & Boyne M.S. (2018). Glutathione metabolism in type 2 diabetes and its relationship with microvascular complications and glycemia. PLoS ONE, 13(6), e0198626.
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2

Analysis of DNA/RNA Adducts by LC-MS

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Reduced L-glutathione (GSH), (2-chloroethyl)-ethyl sulfide (CEES) and L-cysteine (Cys) were purchased from Sigma Aldrich (Saint Quentin Falavier, France). N-Acetyl-L-cysteine (NAC) was purchased from Roche (Boehringen Mannheim) (Mannheim, Germany). 2'-Deoxyguanosine (dGuo) was purchased from Pharma Waldhof (Düsseldorf, Germany). Formic acid (LC-MS grade) was purchased from Thermo Fisher Scientific (Rockford, USA), acetonitrile (HPLC-MS grade) from VWR (Fontenay-sous-Bois, France), methanol (HPLC grade) and ammonium formate from Sigma Aldrich (Saint Quentin Falavier, France). Ribonuclease T1, ribonuclease A, phosphodiesterase II, deoxyribonuclease II, alkaline phosphatase and nuclease P1 were obtained from Sigma. Protease was purchased from Qiagen and phosphodiesterase I from Worthington (Lakewood, NJ, USA). Isotopically labelled molecules Cys* ( 13 C3 99%, 15 N 99%), NAC* ( 13 C3 97-99% 15 N 97-99%), GSH* ( 13 C2 98% 15 N 96-99%) and dGuo* ( 15 N5 98%) were purchased from Cambridge Isotope Laboratories (Andover, USA).
Roser M., Béal D., Eldin C., Gudimard L., Caffin F., Gros-Désormeaux F., Léonço D., Fenaille F., Junot C., Piérard C., & Douki T. (2021). Glutathione conjugates of the mercapturic acid pathway and guanine adduct as biomarkers of exposure to CEES, a sulfur mustard analog. Analytical and Bioanalytical Chemistry, 413(5), 1337-1351.
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3

Analysis of DNA/RNA Adducts by LC-MS

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Reduced L-glutathione (GSH), (2-chloroethyl)-ethyl sulfide (CEES) and L-cysteine (Cys) were purchased from Sigma Aldrich (Saint Quentin Falavier, France). N-Acetyl-L-cysteine (NAC) was purchased from Roche (Boehringen Mannheim) (Mannheim, Germany). 2'-Deoxyguanosine (dGuo) was purchased from Pharma Waldhof (Düsseldorf, Germany). Formic acid (LC-MS grade) was purchased from Thermo Fisher Scientific (Rockford, USA), acetonitrile (HPLC-MS grade) from VWR (Fontenay-sous-Bois, France), methanol (HPLC grade) and ammonium formate from Sigma Aldrich (Saint Quentin Falavier, France). Ribonuclease T1, ribonuclease A, phosphodiesterase II, deoxyribonuclease II, alkaline phosphatase and nuclease P1 were obtained from Sigma. Protease was purchased from Qiagen and phosphodiesterase I from Worthington (Lakewood, NJ, USA). Isotopically labelled molecules Cys* ( 13 C3 99%, 15 N 99%), NAC* ( 13 C3 97-99% 15 N 97-99%), GSH* ( 13 C2 98% 15 N 96-99%) and dGuo* ( 15 N5 98%) were purchased from Cambridge Isotope Laboratories (Andover, USA).
Roser M., Béal D., Eldin C., Gudimard L., Caffin F., Gros-Désormeaux F., Léonço D., Fenaille F., Junot C., Piérard C, & Douki T. (2021). Glutathione conjugates of the mercapturic acid pathway and guanine adduct as biomarkers of exposure to CEES, a sulfur mustard analog. Analytical and bioanalytical chemistry, 413(5).
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