HeLa cells were plated and allowed to adhere and proliferate for 24 h. Cells were treated with compounds for 18 h and harvested on ice. Krishan’s reagent was used to stain the DNA [27 (link)]. The cell cycle distribution was evaluated using a BD FACSCalibur (BD Biosciences, San Jose, CA, USA) and data were analyzed using the FlowJo v10.2 single cell analysis software (FlowJo LLC, Ashland, OR, USA). Quantification of the concentration-dependent effects of compounds on G2/M accumulation was performed using the Muse Cell Analyzer (Millipore, Billerica, MA, USA).
V10.2 single cell analysis software
Manufactured by FlowJo
Sourced in United States
FlowJo v10.2 is a single cell analysis software. It provides tools for comprehensive data analysis and visualization of flow cytometry data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using v10.2 single cell analysis software
1
Cell Cycle Analysis of Compound Effects
2
Cell Cycle Analysis of Compound Effects
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HeLa cells were plated and allowed to adhere and proliferate for 24 h. Cells were treated with compounds for 18 h and harvested on ice. Krishan’s reagent was used to stain the DNA [27 (link)]. The cell cycle distribution was evaluated using a BD FACSCalibur (BD Biosciences, San Jose, CA, USA) and data were analyzed using the FlowJo v10.2 single cell analysis software (FlowJo LLC, Ashland, OR, USA). Quantification of the concentration-dependent effects of compounds on G2/M accumulation was performed using the Muse Cell Analyzer (Millipore, Billerica, MA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!