Sc 352x

Manufactured by Santa Cruz Biotechnology

Sourced in United States

The Sc-352X is a lab equipment product manufactured by Santa Cruz Biotechnology. It is a high-performance centrifuge designed for a variety of laboratory applications. The Sc-352X features a compact and ergonomic design, with a robust construction that ensures reliable operation. Its key function is to separate different components of a sample through the application of centrifugal force.

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Lab products found in correlation

Proteinase k, by Thermo Fisher Scientific (2 mentions) Ab8895, by Abcam (1 mentions) Ab992, by Abcam (1 mentions) Ab70303, by Abcam (1 mentions) Ab26721, by Abcam (1 mentions) Ab9050, by Abcam (1 mentions) Ab31387, by Abcam (1 mentions) Phenol chloroform, by Thermo Fisher Scientific (1 mentions) Protein a sepharose beads, by Thermo Fisher Scientific (1 mentions) Sfx250, by Emerson (1 mentions) G7126, by Merck Group (1 mentions) Sc 150x, by Santa Cruz Biotechnology (1 mentions) Anti irf 1 antibody, by Santa Cruz Biotechnology (1 mentions)

3 protocols using sc 352x

ChIP-qPCR for Histone Modifications

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ChIP was performed as described before.^{26 (link)} In brief, cells were fixed in 0.8% formaldehyde for 6 min at room temperature. Sonicated chromatin was immunoprecipitated with antibodies for H3K4me1 (ab8895; Abcam, Cambridge, MA, USA), H3K36me3 (ab9050; Abcam), RNA polymerase II CTD (ab26721; Abcam), Rad21 (ab992; Abcam), PU.1 (sc-352X; Santa Cruz), CREB (ab31387; Abcam), Hoxa9 (sc-17155X; Santa Cruz Biotechnology, Santa Cruz, CA, USA), CTCF (ab70303; Abcam), or rabbit IgG (15006; Sigma-Aldrich). A 10% aliquot was removed as an input fraction. Quantitative real-time PCR was performed with ChIP DNA and input DNA using a Light Cycler 480II. Relative quantitation was carried out by the comparative threshold cycle (CT) method. Statistical analysis was performed using the GraphPad Prism 5 software. The Student's t-test was used on measurements of enrichment from different condition samples from three experimental replicates. Primers for ChIP-qPCR are shown in Table 1.

Zhang J., Han B., Li X., Bies J., Jiang P., Koller R.P, & Wolff L. (2016). Distal regulation of c-myb expression during IL-6-induced differentiation in murine myeloid progenitor M1 cells. Cell Death & Disease, 7(9), e2364-.

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Chromatin Immunoprecipitation from Mouse Hippocampus

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Hippocampi from male mice were collected as previously described (Gjoneska et al., 2015 (link)) immediately after euthanasia. ChIP was then performed as described in Gjoneska et al. (2015) (link). In brief, tissues were minced and crosslinked in 1% formaldehyde (28906; Thermo Fisher Scientific) for 15 min at room temperature and quenched with glycine for 5 min (g7126; Sigma-Aldrich). The samples were homogenized in cell lysis buffer containing proteinase inhibitors (C762Q77; Thermo Fisher Scientific) and chromatin was then fragmented to a size range of 200–500 bp using a digital sonifier (SFX 250; Branson). Solubilized chromatin was then diluted and incubated with 1 µg antibody (sc-352x; Santa Cruz) at 4°C overnight. Immune complexes were captured with Protein A sepharose beads (101041; Thermo Fisher Scientific), washed, and eluted. Enriched chromatin was then subjected to crosslink reversal and proteinase K (25530049; Thermo Fisher Scientific) digestion at 65°C, phenol-chloroform (17908; Thermo Fisher Scientific) extraction, and ethanol precipitation.

Ralvenius W.T., Mungenast A.E., Woolf H., Huston M.M., Gillingham T.Z., Godin S.K., Penney J., Cam H.P., Gao F., Fernandez C.G., Czako B., Lightfoot Y., Ray W.J., Beckmann A., Goate A.M., Marcora E., Romero-Molina C., Ayata P., Schaefer A., Gjoneska E, & Tsai L.H. (2023). A novel molecular class that recruits HDAC/MECP2 complexes to PU.1 motifs reduces neuroinflammation. The Journal of Experimental Medicine, 220(11), e20222105.

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ChIP-Seq Analysis of Transcription Factors

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In this study, we generated ChIP-seq data from PR9 and PR9+Zn cells for TFs of P300, PU.1, CEBPB, and IRF1, using the following antibodies: anti-P300 (Abcam, ab14984), anti-PU.1 (Santa Cruz, sc-352X), anti-IRF1 antibody (Santa Cruz, sc-497x), and anti-CEBPB antibody (Santa Cruz, sc-150x), and followed standard ChIP-seq protocol [8 (link)].

Wang P., Tang Z., Lee B., Zhu J.J., Cai L., Szalaj P., Tian S.Z., Zheng M., Plewczynski D., Ruan X., Liu E.T., Wei C.L, & Ruan Y. (2020). Chromatin topology reorganization and transcription repression by PML-RARα in acute promyeloid leukemia. Genome Biology, 21, 110.

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