The following chemicals were purchased from Sigma‐Aldrich, St. Louis, MO: Apomorphine (A4393‐1G), 3,3′‐diaminobenzidine (DAB, D5905), ExtrAvidin Peroxidase Staining Kit (EXTRA2), Bovine serum albumine (BSA, A2058) and anti‐TH antibody (T2928), Triton X‐100 (X100), and 6‐OHDA (H116). Antibodies against Syntenin‐1, MFG‐E8, and LGR5 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti‐HSP 70 antibody was from BD Transduction Laboratories (Becton, Dickinson and Company, Franklin Lakes, NY). Artificial cerebrospinal fluid (aCSF) was prepared ex tempore. Apomorphine was dissolved in saline prior to the injection.
6 ohda h116
Manufactured by Merck Group
Sourced in United States
6-OHDA (H116) is a chemical compound used in research applications. It functions as a neurotoxin, specifically targeting and damaging dopaminergic neurons. This product is intended for use in scientific research settings.
Automatically generated - may contain errors
Lab products found in correlation
Syntenin 1, by Santa Cruz Biotechnology (1 mentions)
Mfg e8, by Santa Cruz Biotechnology (1 mentions)
Penicillin streptomycin p s, by Beyotime (1 mentions)
Female wistar rats, by Janvier Labs (1 mentions)
Stereoscopic frame, by Stoelting (1 mentions)
Gsk j4, by Selleck Chemicals (1 mentions)
Dznep hcl, by Selleck Chemicals (1 mentions)
Gsk j1, by Selleck Chemicals (1 mentions)
Gsk126, by Cayman Chemical (1 mentions)
Cpi 455, by Selleck Chemicals (1 mentions)
7 protocols using 6 ohda h116
1
Immunohistochemical Analysis of Dopaminergic Neurons
2
6-OHDA-Induced Cell Stress Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The MES23.5 and MN9D DA cell lines, and 293T cells were obtained from the Shanghai Institutes for Cell Resource Center at the Chinese Academy of Sciences (Shanghai, China). All cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM/High) supplemented with 10% foetal bovine serum (Hyclone, Logan, UT, USA) and penicillin/streptomycin (PS) (Beyotime Biotechnology, Shanghai, China) in a 5% CO2 atmosphere at 37°C. The 6-OHDA (H116) were purchased from Sigma (St. Louis, MO, USA). Cell lines were treated with 6-OHDA (100 μM) for different times (30 min, 1 h, 3 h).
3
Unilateral 6-OHDA Lesion Model in Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female Wistar rats (200–270 g; Janvierlabs, France) were anesthetized with Isoflurane (75% N2O, 20% O2, 4.5–5%) followed by an intraperitoneal (i.p.) injection of Narketan (75 mg/kg; Vétoquind AG, Ittigen, CH, Switzerland) and Xylaxine (5 mg/kg; Vétoquind AG, Ittigen, CH, Switzerland) and a subcutaneous (s.c.) injection of Buprenorphine (0.5 mg/kg; Reckitt Benckiser AG, Wallisellen, Switzerland) applied 30 min before surgical intervention. Thereafter, the rats were placed in a stereoscopic frame (Stoelting Co.) on a heating pad. Each rat received an injection of 4 μl of 6-OHDA (20 mM 6-OHDA; H116 Sigma-Aldrich Chemie GmbH) through a small burr hole created in the skull into the right striatum. The injection was performed over 4 min using a 10 μl Hamilton syringe. The following coordinates in relation to bregma were used (Paxinos Watson rat brain atlas): anterior 1.0 mm, lateral 3.0 mm and 5.0 mm ventral to the dura, the incisor bar was set at 0.0 mm. After the surgery rats were allowed to recover for 5 weeks.
4
6-OHDA-Induced Parkinson's Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CAPE (C8221) and 6-OHDA (H116; contains 0.01% (w/v) ascorbic acid as stabilizer) were purchased from Sigma-Aldrich Corporation, St. Louis, MO, USA. CAPE was dissolved in ethanol and further dilutions were made in saline. Chemicals used for histological verification were purchased from Sigma (Germany). TH was obtained from Bioss, USA (bs-0016R).
5
Dopaminergic Neuron Immunohistochemistry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The 6-OHDA (H116) and GDNF (G1401) were purchased from Sigma (St. Louis, MO, USA). Rabbit anti-Six2 (SAB2103006), rabbit anti-GDNF antibody (SAB1401150), mouse anti-tyrosine hydroxylase (TH) (T2928), and rabbit anti-Smurf1 antibody (S8449) were also from Sigma. The rabbit anti-Six2 antibody (sc-135274) was from Santa Cruz (St. Louis, MO, USA).The rabbit anti-p53 antibody (BS3736) was from Bioworld Technology (Shanghai, China). The plasmid mini kit (74104) and midi kit (12143) were from Qiagen (Venlo, The Netherlands).
6
Intranasal hNSCs Therapy in 6-OHDA-Induced PD Rat Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The animal protocol was approved by the Institutional Ethics Committee of the Second Affiliated Hospital of Soochow University (201511A113). Adult Sprague Dawley rats of both genders (180 ± 10 g) were sourced from Shanghai SLAC Laboratory Animal Co., Ltd (Shanghai, China). The rats were accommodated (five rats per cage) in a temperature-controlled room (22–25°C) with 12 h light/dark cycle. Food and water were offered to the rats without limitation. All animal procedures adhered to the established guidelines of the Animal Use and Care Committee of Soochow University. Fifty-six rats were used to build PD models by 6-hydroxydopamine (6-OHDA, H116, Sigma, USA) injection and 42 rats were successfully modeled. The rats were allocated to four groups at random: (1) sham group (n = 10), (2) PD model group (6-OHDA, n = 14, male, 7; female, 7), (3) low-dose hNSCs group (2 × 105 hNSCs, n = 9, male, 4; female, 5), and (4) high-dose hNSCs group (1 × 106 hNSCs, n = 19, male, 9; female, 10). All rats in (2), (3), and (4) groups were given saline or hNSCs suspension intranasally once a week for 4 weeks. After the first hNSCs/saline treatment, apomorphine (APO)-induced rotation test, stepping test, and open field test were performed every 2 weeks for 12 weeks.
7
Epigenetic Modulation in Cell Experiments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!