Rea control pe

Manufactured by Miltenyi Biotec

REA Control-PE is a control reagent used in flow cytometry analysis. It provides a reference population for setting instrument parameters and gating strategies. The reagent contains PE-conjugated antibodies that bind to a specific cell surface marker, allowing for the identification of positive and negative cell populations.

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Lab products found in correlation

Rea control fitc, by Miltenyi Biotec (1 mentions) Facscalibur, by BD (1 mentions) Accuri c6, by BD (1 mentions) Annexin 5 apc 7 aad apoptosis kit, by Abnova (1 mentions)

2 protocols using rea control pe

Quantifying Pluripotent and Cardiac Cell Populations

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Cells were treated with the appropriate dissociation solution (Accutase for iPS cells, 0.25% trypsin for CMs), fixed with 4% paraformaldehyde, and permeated with 0.1% Triton X-100 at room temperature. Pluripotent stem cells were counted and stained using phycoerythrin conjugated antibodies against SSEA-4 (1:50 dilution; Miltenyi Biotec, Germany) for 30 minutes at 4 °C shielded from light. Cardiomyocytes were co-incubated with anti-cTnT-FITC (1:50 dilution; Miltenyi Biotec) and anti-MLC2v-PE (1:50 dilution; Miltenyi Biotec) in PBS at 4 °C for 30 minutes. REA Control-FITC (1:50 dilution; Miltenyi Biotec) and REA Control-PE (1:50 dilution; Miltenyi Biotec) were used as isotype controls. The cells were measured using FACSCalibur (BD, USA) and subsequent analyses were performed using FlowJo software (Tree Star, version 10.5.3).

Zhu Y., Wang L., Cui C., Qin H., Chen H., Chen S., Lin Y., Cheng H., Jiang X, & Chen M. (2021). Pathogenesis and drug response of iPSC-derived cardiomyocytes from two Brugada syndrome patients with different Nav1.5-subunit mutations. Journal of Biomedical Research, 35(5), 395-407.

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Flow Cytometry Analysis of Apoptosis, Stemness, and Cell Cycle

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An Annexin V-APC/7-AAD Apoptosis Kit (Abnova, #KA3808) was used for the apoptosis analysis as described previously [18 (link)]. ALDH assays were performed as described previously [22 (link)]. Cell cycle assays were performed as described, and propidium iodide(30 μg/ml) was used as DNA content dye [16 (link), 32 (link)]. For CD117 staining, 1 × 10⁶ primary culture tumor cells were stained based on a protocol recommended by the manufacturer. The anti-mouse CD117-PE(Miltenyi Biotec, #REA791) antibody was used. The REA control-PE (Miltenyi Biotec, # REA293) was used as a background control. A BD Accuri C6 flow cytometer (BD Biosciences, San Jose, CA) was used for the analysis. Results were analyzed by the Flow Jo software (Version 10.1, OR, USA). All experiments were performed in triplicates.

Wang J., Ferrena A., Zhang R., Singh S., Viscarret V., Al-Harden W., Aldahamsheh O., Borjihan H., Singla A., Yaguare S., Tingling J., Zi X., Lo Y., Gorlick R., Schwartz E.L., Zhao H., Yang R., Geller D.S., Zheng D, & Hoang B.H. (2024). Targeted inhibition of SCFSKP2 confers anti-tumor activities resulting in a survival benefit in osteosarcoma. Oncogene, 43(13), 962-975.

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