Lc3 1 2

Manufactured by Proteintech

Sourced in United States

LC3-I/II is a primary antibody that recognizes the microtubule-associated protein 1 light chain 3 (LC3) protein. LC3 exists in two forms, LC3-I and LC3-II, which are involved in the process of autophagy. This antibody can be used to detect and quantify the levels of both LC3-I and LC3-II in biological samples.

Automatically generated - may contain errors

Lab products found in correlation

β actin, by Proteintech (2 mentions) α sma, by Proteintech (2 mentions) Beclin 1, by Proteintech (2 mentions) Tgf β1, by Cell Signaling Technology (1 mentions) Bergenin, by Merck Group (1 mentions) Timp1, by Proteintech (1 mentions) Pparγ, by Cell Signaling Technology (1 mentions) P smad2 3, by Cell Signaling Technology (1 mentions) Smad2, by Cell Signaling Technology (1 mentions) Smad3, by Cell Signaling Technology (1 mentions) Sybr premix ex taq, by Takara Bio (1 mentions) Gapdh ap0063, by Bioworld Technology (1 mentions) Bnip3 ab109362, by Abcam (1 mentions) Pgc1α sc 13067, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Beyotime (1 mentions) No 14695 1 ap, by Proteintech (1 mentions) No 14395 1 ap, by Proteintech (1 mentions) No 21898 1 ap, by Proteintech (1 mentions) Tom20, by Proteintech (1 mentions) Tim23, by Proteintech (1 mentions)

7 protocols using lc3 1 2

Bergenin Ameliorates Liver Fibrosis in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Bergenin (CAS: 477-90-7, purity ≥ 98.0%) was purchased from Sigma-Aldrich and dissolved in physiological saline. Primary antibodies α-SMA, CoI-I, TIMP1, LC3-I/II, Beclin-1, and β-actin were acquired from Proteintech Group (Chicago, IL, USA), and PPAR-γ, RXR-α, TGF-β1, Smad2, Smad3, and p-Smad2/3 were from Cell Signaling Technology (Danvers, MA, USA). SYBR Premix Ex Taq was purchased from TaKaRa Biotechnology (Dalian, China).
A total of 64 mice were randomly divided into the CCl₄ model group and the BDL model group, and serum and liver tissue samples were obtained as follows:
Sham operation group (sham,n = 8): intragastric administration of normal saline.
Model group (CCl₄ or BDL,n = 8): model established as above.
Low dose group (CCl₄/BDL+B20,n = 8): daily gavage, Bergenin (20 mg/kg).
High dose group (CCl₄/BDL+B40,n = 8): daily gavage, Bergenin (40 mg/kg).

Xia Y., Li J., Chen K., Feng J, & Guo C. (2020). Bergenin Attenuates Hepatic Fibrosis by Regulating Autophagy Mediated by the PPAR-γ/TGF-β Pathway. PPAR Research, 2020, 6694214.

+ Open protocol

+ Expand

Muscle Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

For protein content analysis, skeletal (quadriceps femoris) and cardiac (left ventricle) muscle samples were homogenized and analyzed by Western blotting. The following antibodies were used: BNIP3 (ab109362, rabbit, 1 : 1000), p-AMPK (ab131357, rabbit, 1 : 500) from Abcam (USA); PGC-1α (sc-13067, rabbit, 1 : 200) from Santa Cruz (USA); CS (16131-1-AP, rabbit, 1 : 1000), LC 3 I/II (14600-1-AP, rabbit, 1 : 500), AMPK (10929-2-AP, rabbit, 1 : 500), DRP1 (12957-1-AP, rabbit, 1 : 500), MFN1 (13798-1-AP, rabbit, 1 : 500), and p62 (18420-1-AP, rabbit, 1 : 1000) from Proteintech (USA); and GAPDH (AP0063, rabbit, 1 : 5000) from Bioworld (USA). Membranes were analyzed and quantified using the Quantity One Imaging System (BIO-RAS, USA). Protein expression was normalized to that of GAPDH.

Dun Y., Liu S., Zhang W., Xie M, & Qiu L. (2017). Exercise Combined with Rhodiola sacra Supplementation Improves Exercise Capacity and Ameliorates Exhaustive Exercise-Induced Muscle Damage through Enhancement of Mitochondrial Quality Control. Oxidative Medicine and Cellular Longevity, 2017, 8024857.

+ Open protocol

+ Expand

Comprehensive Cardiac Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heart tissues or cultured cells were sonicated in RIPA lysis buffer and homogenized. The debris was removed and the supernatant was obtained after centrifugation at 12,000 g for 10 min at 4°C. About 30–50 μg proteins was loaded for electrophoresis, and probed with primary antibodies against collagen I (1:1000; No.14695-1-AP; Proteintech Co., Wuhan, China), α-SMA (1:1000; No.14395-1-AP; Proteintech Co.), TGF-β (1:1000; No.21898-1-AP; Proteintech Co.), LC3 I/II (1:1000; No.14600-1-AP; Proteintech Co., Wuhan, China), TIM23 (1:1000; No.11123-1-AP; Proteintech Co., Wuhan, China), TOM20 (1:1000; No.66777-1-Ig; Proteintech Co., Wuhan, China), OPTN (1:1000; No.10837-1-AP; Proteintech Co., Wuhan, China), COX4 (1:1000; No.11242-1-AP; Proteintech Co., Wuhan, China). GAPDH (1:1000, AF0006; Beyotime Biotechnology Co., Shanghai, China) was used as internal control. Images were analyzed using the Image-Pro Plus software.

Li Y., Zhao K., Hu Y., Yang F., Li P, & Liu Y. (2024). MicroRNA-142-3p alleviated high salt-induced cardiac fibrosis via downregulating optineurin-mediated mitophagy. iScience, 27(5), 109764.

+ Open protocol

+ Expand

Western Blot Analysis of UPF1, mTOR, and Autophagy Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell lysis was taken by RIPA buffer (Beyotime, China) containing PMSF. We separated aliquots of protein by 8% SDS-PAGE, and then electro-transfer to PVDF membrane (Millipore, USA). Subsequently, the membrane was blocked and the designated primary antibody was added overnight at 4° C. The next day, incubated the membrane with the secondary antibody for 2 hours. chemiluminescence assays (Pierce, USA) was used to observe signal and by a ChemiDoc-XRS + (Bio-Rad, CA), we detected and quantified protein. the band detection was within the linear range. Antibodies against UPF1 (Abcam, USA), mTOR, phospho-mTOR, P70S6K, phospho-P70S6K,4EBP1 and phospho-4EBP1 were purchased from Proteintech Technology (Wuhan, China). And autophagy related proteins, LC3I/II, p62, beclin1, atg5 and atg7 were also purchased from Proteintech Technology (Wuhan, China).

Zhang M., Chen H., Qin P., Cai T., Li L., Chen R., Liu S., Chen H., Lin W., Chen H., Strickland A.L., Xiong H, & Jiang Q. (2021). UPF1 impacts on mTOR signaling pathway and autophagy in endometrioid endometrial carcinoma. Aging (Albany NY), 13(17), 21202-21215.

+ Open protocol

+ Expand

Protein Expression Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular proteins (60 μg) were transferred onto a polyvinylidene fluoride membrane (Bio-Rad Laboratories), and after being blocked by 5% fat-free milk for 1.5 h, the membranes were incubated with the following primary antibodies: active caspase-3 (1:1,000, CST), LC3I/II (1:1,000, Proteintech Group, Inc.), and GAPDH (Cat: RT1210-1, Huabio) overnight at 4°C. Then, the membranes were incubated with a secondary antibody for 1 h. The signals were visualized with the ChemiDoc^TM XRS + Imaging System (Bio-Rad Laboratories, Hercules, CA, United States).

Zhang M., Lin J., Jin J., Yu W., Qi Y, & Tao H. (2021). Delivery of siRNA Using Functionalized Gold Nanorods Enhances Anti-Osteosarcoma Efficacy. Frontiers in Pharmacology, 12, 799588.

+ Open protocol

+ Expand

Histological Analysis of Xenograft Tumors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tissue samples isolated from xenograft tumours were subjected to histological analysis. Briefly, tissue samples were first fixed with formalin and then embedded in paraffin, cut into 5 µm sections and stained with H&E for IHC staining. IHC staining was conducted in a DAKO Autostainer system (Dako, Glostrup, Denmark). The primary antibodies used were Bcl-2 (Abcam, ab32124), Ki-67 (Abcam, Cambridge, MA, UK), LC3-I/II (Proteintech, 14600-1-AP), p62 (MBL, M162-3). Primary tumours and major organ sections were stained with H&E. Images were visualised using an Olympus microscope (Japan), and image analysis was performed by Image-Pro Plus 6.0.

Gao L., Wang Z., Lu D., Huang J., Liu J, & Hong L. (2019). Paeonol induces cytoprotective autophagy via blocking the Akt/mTOR pathway in ovarian cancer cells. Cell Death & Disease, 10(8), 609.

+ Open protocol

+ Expand

Protein Extraction and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Harvested protein from clinical sample tissue (grind with a homogenizer on ice)/cell by using the whole protein extraction kit (Keygen, China). Then sodium dodecyl sulfate-polyacrylamide gel electrophoresis was performed and transferred to a polyvinylidene fluoride membrane. Results were quantified by ImageJ software (NIH, United States) after being incubated with the primary antibodies overnight. p-AMPK, AMPK, p-mTOR, mTOR, p-P70S6K, and P70S6K antibodies were from Cell Signaling Technology (Massachusetts, United States), LC3 II/I, SESN2, GAPDH and β-actin were from proteintech (State of New Jersey, United States).

Qu N., Qu J., Huang N., Zhang K., Ye T., Shi J., Chen B., Kan C., Zhang J., Han F., Hou N., Sun X, & Pan R. (2022). Calycosin induces autophagy and apoptosis via Sestrin2/AMPK/mTOR in human papillary thyroid cancer cells. Frontiers in Pharmacology, 13, 1056687.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!