Log In Sign up
The largest database of trusted experimental protocols

Spinning disc confocal scanner

Manufactured by PerkinElmer
Visit Supplier

The Spinning Disc Confocal Scanner is a laboratory equipment that utilizes a spinning disc to capture high-speed, high-resolution images. It illuminates the sample with a laser and collects the resulting fluorescent signal through a series of optical components, including a spinning disc with an array of pinholes. This allows for the efficient capture of in-focus images from multiple planes within the sample simultaneously.

Automatically generated - may contain errors

Lab products found in correlation

Inverted microscope, by Zeiss (4 mentions) Alexa fluor conjugated phalloidin, by Thermo Fisher Scientific (2 mentions) Prolong anti fade media, by Thermo Fisher Scientific (2 mentions) T6074, by Merck Group (2 mentions) Triton x 100, by Merck Group (2 mentions)

Close spelling variants of this product

UltraView spinning-disc confocal scanner unit

2 protocols using spinning disc confocal scanner

1

Immunocytochemistry of Cytoskeletal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
NIH/3T3 cells were fixed with 0.25% glutaraldehyde and permeabilized with 0.1% Triton x100 (Sigma). Mouse anti-α-tubulin (T6074, Sigma, 1:5,000) and goat anti-FLAG (A190–101A, Bethyl Laboratories, 1:500) antibodies were incubated overnight at 4 °C. Appropriate secondary AlexaFluor-conjugated antibodies (Life Technologies, 1:1,000) along with AlexaFluor-conjugated phalloidin to visualize F-actin (A12379, Life Technologies, 1:100) were applied for 1 hour at room temperature. Cover glasses were mounted in ProLong anti-fade media (Life Technologies) and visualized with 100x oil objective on inverted microscope (Zeiss) fitted with spinning disc confocal scanner (Perkin-Elmer). Imaging analysis was performed using ImageJ software as follows: Confocal stacks were projected into a single plane (Z-project, Maximal Intensity), images were thresholded and fluorescence intensity measured as a mean gray value. The investigator collecting images was blinded to the experimental groups. During analysis of immunocytochemistry data, the investigator was blinded to the identity of the experimental groups.
Vabres P., Sorlin A., Kholmanskikh S.S., Demeer B., St-Onge J., Duffourd Y., Kuentz P., Courcet J.B., Carmignac V., Garret P., Bessis D., Boute O., Bron A., Captier G., Carmi E., Devauchelle B., Geneviève D., Gondry-Jouet C., Guibaud L., Lafon A., Mathieu-Dramard M., Thevenon J., Dobyns W.B., Bernard G., Polubothu S., Faravelli F., Kinsler V.A., Thauvin C., Faivre L., Ross M.E, & Rivière J.B. (2019). Postzygotic inactivating mutations of RHOA cause a mosaic neuroectodermal syndrome. Nature genetics, 51(10), 1438-1441.
+ Open protocol
+ Expand
2

Immunocytochemistry of Cytoskeletal Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
NIH/3T3 cells were fixed with 0.25% glutaraldehyde and permeabilized with 0.1% Triton x100 (Sigma). Mouse anti-α-tubulin (T6074, Sigma, 1:5,000) and goat anti-FLAG (A190–101A, Bethyl Laboratories, 1:500) antibodies were incubated overnight at 4 °C. Appropriate secondary AlexaFluor-conjugated antibodies (Life Technologies, 1:1,000) along with AlexaFluor-conjugated phalloidin to visualize F-actin (A12379, Life Technologies, 1:100) were applied for 1 hour at room temperature. Cover glasses were mounted in ProLong anti-fade media (Life Technologies) and visualized with 100x oil objective on inverted microscope (Zeiss) fitted with spinning disc confocal scanner (Perkin-Elmer). Imaging analysis was performed using ImageJ software as follows: Confocal stacks were projected into a single plane (Z-project, Maximal Intensity), images were thresholded and fluorescence intensity measured as a mean gray value. The investigator collecting images was blinded to the experimental groups. During analysis of immunocytochemistry data, the investigator was blinded to the identity of the experimental groups.
Vabres P., Sorlin A., Kholmanskikh S.S., Demeer B., St-Onge J., Duffourd Y., Kuentz P., Courcet J.B., Carmignac V., Garret P., Bessis D., Boute O., Bron A., Captier G., Carmi E., Devauchelle B., Geneviève D., Gondry-Jouet C., Guibaud L., Lafon A., Mathieu-Dramard M., Thevenon J., Dobyns W.B., Bernard G., Polubothu S., Faravelli F., Kinsler V.A., Thauvin C., Faivre L., Ross M.E, & Rivière J.B. (2019). Postzygotic inactivating mutations of RHOA cause a mosaic neuroectodermal syndrome. Nature genetics, 51(10), 1438-1441.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!