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Anti δ catenin

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Anti-δ-catenin is a laboratory reagent used for the detection and quantification of delta-catenin protein in biological samples. It is a monoclonal antibody that specifically binds to the delta-catenin protein, which is involved in cell-cell adhesion and signaling pathways. The anti-δ-catenin reagent can be used in various analytical techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of delta-catenin in cells and tissues.

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Lab products found in correlation

Anti e cadherin, by Santa Cruz Biotechnology (3 mentions) Anti e cadherin, by BD (2 mentions) Anti gfp, by Santa Cruz Biotechnology (2 mentions) Anti py20, by Santa Cruz Biotechnology (2 mentions) Anti β actin, by Merck Group (2 mentions) Anti tubulin, by Merck Group (1 mentions) Anti flag, by Merck Group (1 mentions) Anti actin, by Santa Cruz Biotechnology (1 mentions) Anti lamin b, by Santa Cruz Biotechnology (1 mentions) Anti pcaf, by Santa Cruz Biotechnology (1 mentions) Anti myc, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Merck Group (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti p erk1 2, by Santa Cruz Biotechnology (1 mentions) Anti egfr 1005, by Santa Cruz Biotechnology (1 mentions) Anti myc tag 9b11, by Cell Signaling Technology (1 mentions) M mlv reverse transcriptase kit, by Thermo Fisher Scientific (1 mentions) Anti cleaved parp, by Cell Signaling Technology (1 mentions) Epidermal growth factor (egf), by Merck Group (1 mentions) Paclitaxel, by Merck Group (1 mentions)

5 protocols using anti δ catenin

1

Antibody Panel for Protein Analysis

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The following antibodies were used in the present study: anti-δ-catenin (#611537, BD Bioscience, San Jose, CA, USA), anti-GFP (#G1544, Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-tubulin (#T9026, Sigma-Aldrich, St Louis, MO, USA), anti-lamin B (SC-6216, Santa Cruz Biotechnology), anti-ß-actin (Santa Cruz Biotechnology), anti-flag (Sigma-Aldrich), anti-PCAF (SC-13124, Santa Cruz Biotechnology), anti-E-cadherin (BD Bioscience), anti-acetylated-Lys (Cell Signaling, Beverly, MA, USA), anti-p120-δ-catenin (BD Bioscience), anti-autophagy-related protein 5 (Atg5) (Cell Signaling), anti-myc (Santa Cruz Biotechnology), and anti-cyclin-D1 (Calbiochem, San Diego, CA, USA).
Zhou R., Yang Y., Park S.Y., Seo Y.W., Jung S.C., Kim K.K., Kim K, & Kim H. (2019). p300/CBP-associated factor promotes autophagic degradation of δ-catenin through acetylation and decreases prostate cancer tumorigenicity. Scientific Reports, 9, 3351.
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2

Antibody Validation for Immunoblotting and IF

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Antibodies for immunoblotting were from commercially available sources. anti-δ-catenin (#611537, BD Bioscience); anti-GFP (#G1544, Sigma); anti-p-Erk1/2 (#16982, Santa Cruz); anti-Erk1 (SC-94, Santa Cruz); anti-EGFR 1005 (#03, Santa Cruz); anti-EGFR (D20) (#31156, Santa Cruz) anti-E-cadherin (SC-7870, Santa Cruz); anti-py20 (SC-508, Santa Cruz) and anti-Myc-Tag (9B11) (#2276,Cell signaling).
Antibodies for immunofluorescence were also obtained commercially: anti-E-cadherin (#3195, Cell signaling), anti-EGFR 225 (MA5-12880, ThermoFisher Scientific).
Control siRNA and siRNA anti-δ-catenin were purchased from Sigma-Aldrich.
He Y., Ryu T., Shrestha N., Yuan T., Kim H., Shrestha H., Cho Y.C., Seo Y.W., Song W.K, & Kim K. (2016). Interaction of EGFR to δ-catenin leads to δ-catenin phosphorylation and enhances EGFR signaling. Scientific Reports, 6, 21207.
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3

Anti-Cancer Agent Screening Protocol

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The anti-δ-catenin (#611537) antibody was purchased from BD Bioscience (USA). The anti-GFP (#G1544) and anti-β-actin (#A5441) antibodies were bought from Sigma-Aldrich (USA). The anti-EGFR (1005) (#SC-03), anti-c-Cbl (C-15) (#SC-170), anti-Erk (C-140) (#SC-154) and anti-E-cadherin (SC-7870) antibodies were purchased from Santa Cruz Bio-technology, Inc., USA. The anti-p-Erk (P-p44/42 MAPK) (#9101S), anti-α-tubulin (#T9026) and anti-cleaved PARP (#9541) antibodies were from Cell Signaling Technology (USA). The HA epitope was detected using media from 12CA5 hybridomas provided by Professor Kwang Youl Lee from Chonnam National University.
The epidermal growth factor (EGF) (#E9644) was obtained from Sigma-Aldrich (USA). Paclitaxel (#T7191) and MTS solution (#G3580) were purchased from Sigma-Aldrich (USA) and Promega Biotechnology company (USA) respectively. The M-MLV reverse transcriptase kit was obtained from Invitrogen (USA). The SYBR green was bought from Enzynomics (Korea). The CFX System was from Bio-Rad (USA).
Shrestha N., Shrestha H., Ryu T., Kim H., Simkhada S., Cho Y.C., Park S.Y., Cho S., Lee K.Y., Lee J.H, & Kim K. (2018). δ-Catenin Increases the Stability of EGFR by Decreasing c-Cbl Interaction and Enhances EGFR/Erk1/2 Signaling in Prostate Cancer. Molecules and Cells, 41(4), 320-330.
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4

Antibody Validation for Western Blotting

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The following antibodies were purchased from commercial companies: anti-δ-catenin (#611537, BD Bioscience, Franklin Lakes, NJ, USA), anti-p21Waf1/Cip1 (#SC6246, Santa Cruz Biotechnology, Dallas, TX, USA), anti-phospho-p21Thr 145 (#SC-377569, Santa Cruz Biotechnology, Dallas, TX, USA), anti-AKT (#4691, Cell Signaling Technology, Danvers, MA, USA), anti-phospho-AKTSer 473 (#4060, Cell Signaling Technology, Danvers, MA, USA), anti-GFP (#SC9996, Santa Cruz Biotechnology, Dallas, TX, USA), anti-LamB (#13435, Cell Signaling Technology, Danvers, MA, USA), and anti-β-actin (#A5441, Sigma-Aldrich, St. Louis, MO, USA).
Shen Y., Lee H.J., Zhou R., Kim H., Chen G., Cho Y.C, & Kim K. (2021). δ-Catenin Participates in EGF/AKT/p21Waf Signaling and Induces Prostate Cancer Cell Proliferation and Invasion. International Journal of Molecular Sciences, 22(10), 5306.
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5

Antibody Sourcing and Detection

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Antibodies were purchased from commercial companies as follows: anti-δ-catenin (#611537), anti-E-cadherin (#610182, BD Bioscience); anti-GFP (#G1544), anti-β-actin (#A5441), anti-β-catenin (#C2206), and anti-α-tubulin (#T9026,sigam) anti-Lamin B (SC-6216), anti-py20 (SC-508) and anti-E-cadherin (SC-7080, Santa Cruz); anti-Src (#05-184), anti-Fyn (#4023), anti-Fgr (#2755), anti-Lyn (#2732), anti-myc (#2276) and anti-GSK3α/β(#5676, Cell Signaling). HA epitope was detected using media from 12CA5 hybridoma.
He Y., Kim H., Ryu T., Lee K.Y., Choi W.S., Kim K.M., Zheng M., Joh Y., Lee J.H., Kwon D.D., Lu Q, & Kim K. (2014). C-Src-mediated phosphorylation of δ-catenin increases its protein stability and the ability of inducing nuclear distribution of β-catenin. Biochimica et biophysica acta, 1843(4), 758-768.
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