Akt inhibitor x

Manufactured by Cayman Chemical

Sourced in United States

AKT inhibitor X is a small molecule that inhibits the activity of the AKT protein kinase. AKT is a key regulator of cellular processes such as metabolism, proliferation, and survival. AKT inhibitor X can be used in research applications to study the role of AKT in various biological systems.

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Lab products found in correlation

Oil red o, by Merck Group (2 mentions) Facsuite software, by BD (1 mentions) Bms 345541, by Abcam (1 mentions) Facsverse, by BD (1 mentions) Fitc brdu flow kit, by BD (1 mentions) Annexin 5 fitc, by BD (1 mentions) Ly294002, by Fujifilm (1 mentions) Normal goat igg, by Santa Cruz Biotechnology (1 mentions) 3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions) Baicalein, by Cayman Chemical (1 mentions) 2 deoxy 2 7 nitro 2 1 3 benzoxadiazol 4 yl amino d glucose 2 nbdg, by Cayman Chemical (1 mentions) Horseradish peroxidase conjugated anti rabbit or anti mouse igg antibody, by Santa Cruz Biotechnology (1 mentions) Insulin, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Monoclonal anti β actin, by Merck Group (1 mentions) Recombinant human insulin, by Merck Group (1 mentions) Wortmannin, by Merck Group (1 mentions)

5 protocols using akt inhibitor x

Osteoclast Formation and Function Assays

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BMMs were cultured in the presence of macrophage colony-stimulating factor (M-CSF) (20 ng/ml) and RANKL (20 ng/ml) for 5 days, and TRAP staining and the pit formation assay were performed as previously described (63 (link), 64 (link)). Cells were treated with BMS-345541 (Abcam) at 0.16 μM or Akt inhibitor X (Cayman) at 0.2 μM followed by TRAP staining. Cells were cultured with 10 μM BrdU for 45 min and were prepared for analysis of BrdU incorporation using the FITC BrdU Flow Kit (BD Biosciences) by flow cytometric analysis using the FACSVerse (BD Biosciences). Cell death assay was performed with FITC–Annexin V (BD Biosciences) and propidium iodide by FACSVerse. Data were analyzed by FACSuite software (BD Biosciences).

Ozaki K., Yamada T., Horie T., Ishizaki A., Hiraiwa M., Iezaki T., Park G., Fukasawa K., Kamada H., Tokumura K., Motono M., Kaneda K., Ogawa K., Ochi H., Sato S., Kobayashi Y., Shi Y.B., Taylor P.M, & Hinoi E. (2019). The L-type amino acid transporter LAT1 inhibits osteoclastogenesis and maintains bone homeostasis through the mTORC1 pathway. Science signaling, 12(589), eaaw3921.

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Investigating AKT Inhibition in Breast Cancer

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LY294002 (FUJIFILM Wako Pure Chemical, Osaka, Japan) 20 µM, AKT inhibitor X (Cayman Chemical, MI, USA) 20 µM, and AZD-8055 (ChemScene, NJ, USA) 1 µM were added to medium, OC, and vehicle (0.04% DMSO). MDA-MB-231 cells were seeded into 6-well plates at a cell density of 2.0 × 10⁵ cells/ml for 3 days. The cells were with vehicle alone or with OC with added vehicle, LY294002, AKT inhibitor X, or AZD-8055. We performed western blot at 2 weeks and alizarin staining at 6 weeks.

Fushimi A., Takeyama H., Tachibana T, & Manome Y. (2020). Osteogenic cocktail induces calcifications in human breast cancer cell line via placental alkaline phosphatase expression. Scientific Reports, 10, 12669.

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Adipocyte Differentiation Pathway Analysis

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Oil Red O, 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, and insulin were purchased from Sigma (St. Louis, MO, USA). Baicalein, 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]-D-glucose (2-NBDG), and Akt Inhibitor X were from Cayman Chemical (Ann Arbor, MI, USA). Anti-Akt and anti-phospho-Akt (p-Akt; Thr308) polyclonal antibodies were obtained from Cell Signaling (Danvers, MA, USA). Anti-C/EBPα (C-18), anti-AMPKα (H-300), and anti-phospho-AMPKα (p-AMPK; Thr172) polyclonal antibodies and normal goat IgG were from Santa Cruz Biotech. (Santa Cruz, CA, USA). Anti-β-actin (AC-15) monoclonal antibody was from Sigma. Horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG antibody was from Santa Cruz Biotech.

Nakao Y., Yoshihara H, & Fujimori K. (2016). Suppression of Very Early Stage Of Adipogenesis by Baicalein, a Plant-Derived Flavonoid through Reduced Akt-C/EBPα-GLUT4 Signaling-Mediated Glucose Uptake in 3T3-L1 Adipocytes. PLoS ONE, 11(9), e0163640.

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Adipocyte Differentiation and Lipogenesis

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3-Isobutyl-1-methylxanthine (IBMX), dexamethasone (Dex), insulin, and Oil Red O were purchased from Sigma (St. Louis, MO, USA). Akt Inhibitor X was obtained from Cayman Chemical (Ann Arbor, MI, USA). Anti-Akt, anti-phospho-Akt (p-Akt; Thr308), anti-phospho-FOXO1 (p-FOXO1; Ser256), anti-C/EBPα, and anti-hormone-sensitive lipase (HSL) polyclonal antibodies were from Cell Signaling (Danvers, MA, USA). Anti-PPARγ, anti-fatty acid synthase (FAS), and anti-FOXO1 polyclonal antibodies, and normal rabbit IgG, anti-mouse, anti-goat, or anti-rabbit IgG antibody conjugated with horseradish peroxidase (HRP) were from Santa Cruz Biotech. (Dallas, TX, USA). Anti-β-actin monoclonal and anti-glucose transporter 4 (GLUT4) polyclonal antibodies were from Sigma.

Nagai S., Matsumoto C., Shibano M, & Fujimori K. (2018). Suppression of Fatty Acid and Triglyceride Synthesis by the Flavonoid Orientin through Decrease of C/EBPδ Expression and Inhibition of PI3K/Akt-FOXO1 Signaling in Adipocytes. Nutrients, 10(2), 130.

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Biochemical Reagent Procurement and Utilization

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Recombinant human insulin and wortmannin were obtained from Sigma-Aldrich (St. Louis, MO). LY204002 was purchased from Cell Signaling Technology (Danvers, MA). AKT inhibitor X was purchased from Cayman Chemicals (Ann Arbor, MI).

Eichler T., Becknell B., Easterling R.S., Ingraham S.E., Cohen D.M., Schwaderer A., Hains D.S., Li B., Cohen A., Metheny J., Trindandapani S, & Spencer J.D. (2016). Insulin and the PI3K/AKT Signaling Pathway Regulate Ribonuclease 7 Expression in the Human Urinary Tract. Kidney international, 90(3), 568-579.

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