To further validate the identified DEPs, ELISA was performed to measure plasma proteins concentrations according to the manufacturer’s instructions. The concentrations of C-reactive protein (CRP), heat shock protein beta-1 (HSPB1), vinculin (VINC) and growth/differentiation factor 15 (GDF15) were respectively quantified using a Human CRP Quantikine ELISA Kit (DCRP00, R&D Systems), HSPB1 Human ELISA Kit (EHHSPB1, Thermo Fisher Scientific), Human Vinculin ELISA Kit (LS-F34925, LifeSpan Biosciences) and Human GDF15 ELISA Kit (KIT10936, Sino Biological).
Human crp quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human CRP Quantikine ELISA Kit is an in vitro enzyme-linked immunosorbent assay (ELISA) designed for the quantitative measurement of human C-reactive protein (CRP) levels in serum, plasma, and cell culture supernatants.
Automatically generated - may contain errors
Lab products found in correlation
Human cxcl12 sdf 1α quantikine elisa kit, by R&D Systems (2 mentions)
Il 1β human il 1β il 1β f2 quantikine elisa kit, by R&D Systems (2 mentions)
Mouse tnf α duoset elisa kit, by R&D Systems (1 mentions)
Human il 6 quantikine elisa kit, by R&D Systems (1 mentions)
Human tnf alpha quantikine elisa kit, by R&D Systems (1 mentions)
Human il 8 quantikine elisa kit, by R&D Systems (1 mentions)
Quantikine human bdnf kit, by R&D Systems (1 mentions)
Spectramax m2, by Molecular Devices (1 mentions)
7 protocols using human crp quantikine elisa kit
1
Plasma Protein Validation by ELISA
2
Standardized ELISA Methods for CRP and MIA
3
Quantitative Determination of Inflammatory Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The method used for the quantitative determination of CRP, IL-1β and SDF-1α was the enzyme-linked immunosorbent assay (ELISA) method. The protocol for each of the three ILs was performed following the instructions of R&D Systems Human Kit designated for each cytokine in part (Human CRP Quantikine ELISA Kit, IL-1β Human IL-1β/IL-1β F2 Quantikine ELISA Kit and Human CXCL12/SDF-1α Quantikine ELISA Kit, R&D Systems Inc., Minneapolis, MN). The normal range of values for CRP was between 1.09 and 4.291 mg/L, with a mean of 1.769 mg/L, with the limit of detection 0.005–0.022 ng/mL (mean value 0.010 ng/mL) and no significant cross-reactivity or interference; the coefficient of variance for intra-assay precision was 3.8–4.3% and for inter-assay precision 6–7%. For IL-1β, the normal range of values was between 1 and 3.9 pg/mL, with the limit of detection 1 pg/mL and no significant cross-reactivity or interference; the coefficient of variance for intra-assay precision was in the range 2.8–8.5% and for inter-assay precision, 4.1–8.4%. For SDF-1α, the normal range of values was between 1330 and 2720 pg/mL (mean 1830 pg/mL), with the limit of detection 1.0–47 pg/mL (mean value 18 pg/mL) and no significant cross-reactivity or interference; the coefficient of variance for intra-assay precision was between 3.4% and 3.9% and for inter-assay precision, 8.2–13.4%
4
CISD2, IL6, and CRP Quantification in CNS Insults
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The plasma and CSF levels of CISD2, IL6, and CRP in patients with CNS insults were examined using human CISD2 ELISA kit (catalog: MBS7244473, MyBiosource, San Diego, CA, USA), human IL-6 Quantikine ELISA kit (catalog: D6050, R&D Systems, Minneapolis, MN, USA), and human CRP Quantikine ELISA kit (catalog: DCRP00, R&D Systems), respectively, whereas the brain levels of Tnfa in SCI mice were examined using a DuoSet® mouse TNF-α ELISA kit (Catalog: DY410, R&D Systems). Briefly, 300 μL human plasma or CSF samples or mouse brain tissue lysates diluted (1:100) in lysis buffer were subjected to the immunoassay, following the manufacturer’s instructions. The standards of the target protein were analyzed in duplicates, whereas the samples were analyzed in triplicates. At least three independent human samples and six independent mouse samples were used for the analysis.
5
Plasma BDNF and Cytokine Measurement
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plasma BDNF levels were measured using a BDNF Kit (Quantikine Human BDNF kit; R&D Systems, Minneapolis, MN, USA) (www.RnDSystems.com ) and an ELISA reader with a minimum detectable dose of 80 pg/mL (SpectraMax-M2; Molecular Devices, Sunnyvale, CA, USA). All samples were analyzed twice. The cytokine ELISA kits used were also purchased from R&D systems and included a Human CRP Quantikine ELISA Kit, a Human TNF-alpha Quantikine ELISA Kit, and a Human IL-8 Quantikine ELISA Kit for the assessment of plasma levels of CRP, TNF-α, and IL-8, respectively.
6
Quantitative Determination of Inflammatory Biomarkers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The method used for the quantitative determination of CRP, IL-1β and SDF-1α was the enzyme-linked immunosorbent assay (ELISA) method. The protocol for each of the three ILs was performed following the instructions of R&D Systems Human Kit designated for each cytokine in part (Human CRP Quantikine ELISA Kit, IL-1β Human IL-1β/IL-1β F2 Quantikine ELISA Kit and Human CXCL12/SDF-1α Quantikine ELISA Kit, R&D Systems Inc., Minneapolis, MN). The normal range of values for CRP was between 1.09 and 4.291 mg/L, with a mean of 1.769 mg/L, with the limit of detection 0.005–0.022 ng/mL (mean value 0.010 ng/mL) and no significant cross-reactivity or interference; the coefficient of variance for intra-assay precision was 3.8–4.3% and for inter-assay precision 6–7%. For IL-1β, the normal range of values was between 1 and 3.9 pg/mL, with the limit of detection 1 pg/mL and no significant cross-reactivity or interference; the coefficient of variance for intra-assay precision was in the range 2.8–8.5% and for inter-assay precision, 4.1–8.4%. For SDF-1α, the normal range of values was between 1330 and 2720 pg/mL (mean 1830 pg/mL), with the limit of detection 1.0–47 pg/mL (mean value 18 pg/mL) and no significant cross-reactivity or interference; the coefficient of variance for intra-assay precision was between 3.4% and 3.9% and for inter-assay precision, 8.2–13.4%
7
Quantifying Serum C-Reactive Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum levels of C-reactive protein (CRP) were measured using a human CRP quantikine ELISA Kit (DCRP00, R&D Systems). All operations were performed in strict accordance with manufacturer's instructions. Serum levels of CRP were normalized to mg/L.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!