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Bond 3

Manufactured by Roche
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The Bond III is a fully automated analyzer designed for clinical chemistry and immunochemistry testing. It offers high throughput capabilities and is suitable for a wide range of in vitro diagnostic applications in clinical laboratories.

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Lab products found in correlation

Pd l1 ihc 22c3 pharmdx kit, by Agilent Technologies (1 mentions) Sp142, by Roche (1 mentions) Benchmark ultra, by Agilent Technologies (1 mentions) Sp263, by Roche (1 mentions)

3 protocols using bond 3

1

Optimizing PD-L1 IHC Assay Protocols

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PD-L1 IHC using the PD-L1 IHC 22C3 pharmDx kit on the Dako ASL48 platform was performed according to manufacturer recommendations [10 ]. The 22C3 antibody in this kit is provided already diluted at an unspecified ratio. For development of high-quality laboratory-developed tests (LDTs), several technical conditions were evaluated (eg, slide thickness 3 μm versus 4 μm, pre-treatment delays, primary antibody dilution, incubation time, amplification systems) using the 22C3 antibody concentrate on the Dako ASL48, VENTANA BenchMark ULTRA and Bond-III platforms (S1 Table). Details of the optimised protocols are described below.
Ilie M., Khambata-Ford S., Copie-Bergman C., Huang L., Juco J., Hofman V, & Hofman P. (2017). Use of the 22C3 anti–PD-L1 antibody to determine PD-L1 expression in multiple automated immunohistochemistry platforms. PLoS ONE, 12(8), e0183023.
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2

Comprehensive Immunohistochemical Profiling

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Standard basic workup included all diagnostic antibodies: CD20, CD3, CD45, Pax5, CD30, and CD15. In addition, OCT2, CD79a, PD1, and programmed death ligand-1 (PD-L1) (Abcam, rabbit monoclonal, clone 28–8) were performed in a subset of cases. All immunohistochemical work except for p-STAT6Y641 staining was performed in the Clinical Immunohistochemistry Lab of the Pathology Department of University of Chicago Medicine. Immunohistochemical staining for all standard antibodies was performed on 4 μm thick tissue sections using either on the Leica Bond III or Ventana XT instruments with appropriate positive and negative controls. Sections were baked for 3 h at 60 °C in a dehydration oven.
Van Slambrouck C., Huh J., Suh C., Song J.Y., Menon M.P., Sohani A.R., Duffield A.S., Goldberg R.C., Dama P., Kiyotani K., Godfrey J., Fitzpatrick C., Kline J., Smith S.M., Jaffe E.S., Hartmann S, & Venkataraman G. (2019). Diagnostic utility of STAT6YE361 expression in classical Hodgkin lymphoma and related entities. Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 33(5), 834-845.
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3

Standardization of PD-L1 Immunostaining Assays

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For the standardization/training set, we used 5 PD-L1 antibodies and 3 automated immunostaining platforms (Leica Bond III, Ventana Benchmark Ultra, and Dako AS Link 48), thus producing 8 combinations of antibodies and platforms. Four standardized PD-L1 assays were performed: 22C3 (Agilent/Dako, PD-L1 pharmDx assay tested in 2 centers), 28-8 (Agilent/Dako, PD-L1 pharmDx assay tested in 2 centers), SP142 (Ventana, Tucson, AZ, USA), and SP263 (Ventana). Tests were performed in accordance with the manufacturer's protocols. LDTs with clone QR1 (Quartett, Berlin, Germany) were tested on the 3 automated immunostaining platforms; LDTs with clone 22C3 were performed on a Leica Bond platform. These LDTs were previously validated in non-small cell lung cancer (21) .
For the validation set of 40 cases, we selected one protocol on each platform based on the best reproducibility (QR1 Leica Bond, SP263 Ventana, and 28-8 Dako).
Darmon-Novello M., Adam J., Lamant L., Battistella M., Ortonne N., Balme B., de la Fouchardière A., Chaltiel L., Gerard E., Franchet C, & Vergier B. (2022). Harmonization of programmed death-ligand 1 immunohistochemistry and mRNA expression scoring in metastatic melanoma: a multicentre analysis. Histopathology, 80(7).
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