Fifty micrograms of total protein was digested with Trypsin Gold (Promega, UK) in the presence of 8 M urea in 50 mM ammonium bicarbonate pH 8.0 and the surfactant ProteaseMaxTM (Promega). The sample was reduced in 5 mM DTT at 37°C for 1 h, and alkylated in 15 mM iodoacetamide at ambient temperature for 20 min. Trypsin digestion was performed at an enzyme‐to‐protein ratio of 1:25 at 37°C for 16 h. Peptides were collected by centrifugation and acidified with formic acid to a concentration of 0.5%.
Proteasemaxtm
Manufactured by Promega
Sourced in United Kingdom
ProteaseMaxTM is a laboratory product designed to facilitate the extraction and isolation of proteins from biological samples. It serves as a reliable tool for researchers and scientists working in the field of proteomics and protein analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using proteasemaxtm
1
Trypsin-Based Protein Digestion Protocol
2
Protease Activity Assay Protocol
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Materials. Aeromonas proteolytica aminopeptidase (BLAP, PDB 1RTQ, Sigma, 116.51 units mg -1 ), ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA, BioUltra, 99.5-101.5 %), iodoacetamide (IAM, 99 %), DL-dithio-threitol (DTT, 99 %), perinaphthenone (PN, 97 %), 7-hydroxy-4-methylcoumarin, 7-leucine-7-amido-4-methylcoumarin hydrochloride, were purchased from Sigma. Furfuryl alcohol (FFA, Merck, 98%) was distilled prior to use.
ProteaseMAX TM (Promega, lyophilized, V207A), sequencing grade chymotrypsin (Promega, lyophilized, V106A), iRT peptides (iRT Kit, Biognosys) were used for protein digestion and analytical measurements, respectively. Further chemicals purchased from commercial vendors are listed in Section S1.
ProteaseMAX TM (Promega, lyophilized, V207A), sequencing grade chymotrypsin (Promega, lyophilized, V106A), iRT peptides (iRT Kit, Biognosys) were used for protein digestion and analytical measurements, respectively. Further chemicals purchased from commercial vendors are listed in Section S1.
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