Anti fgfr1 fgfr1

Primary antibodies: anti-phospho-FGFR (Tyr653/Tyr654) (p-FGFR) (#06-1433) were from Merck (Darmstadt, Germany); anti-phospho-mTOR (Ser2448) (p-mTOR) (#2971), anti-FGFR1 (FGFR1) (#9740), anti-phospho-EGFR (Y1173) (p-EGFR) (#4407), anti-phospho-AKT (Ser473) (p-AKT p-S473) (#9271), anti-phospho-AKT (Thr308) (p-AKT p-T308) (#9275), anti-AKT1/2/3 (AKT) (#9272), anti-phospho-p44/42 (Thr202/Tyr204) MAP kinase (p-ERK1/2) (#9101), anti-p44/42 MAP kinase (ERK1/2) (#9102), anti-MDR1/ABCB1 (MDR1) (#12683), and anti-poly-[ADP-ribose] polymerase (PARP) (#9542) were from Cell Signaling Technology (Danvers, MA, USA); anti-mTOR (mTOR) (#T2949), anti-γ-tubulin (tubulin) (#T6557) and anti-acetylated-α-tubulin (ac-tubulin) (#T7451) were from Sigma Aldrich (St Louis, MO, USA). Horseradish peroxidase-conjugated secondary antibodies were from Jackson Immuno-Research Laboratories (Cambridge, UK) and a chemiluminescent substrate was used to visualize them in the ChemiDoc station (BioRad, Hercules, CA, USA). AlexaFluor^®594-conjugated secondary antibodies were from Abcam (Cambridge, UK). NucBlue Live ReadyProbes Reagent was from Thermo Fisher (Waltham, MA, USA). Geneticin (G-418) was from BioShop (Puck, Poland). Penicillin-Streptomycin Solution was from Biowest (Nuaille, France). Heparin came from Sigma-Aldrich.

The primary antibodies: anti-phospho-FGFR (Tyr653/Tyr654) (p-FGFR) (#06-1433), anti-FGFR1 (FGFR1) (#9740), anti-phospho-p44/42 (Thr202/Tyr204) MAP kinase antibody (p-Erk1/2) (#9101), anti-p44/42 MAP kinase antibody (Erk1/2) (#9102), anti-caspase 3 (caspase 3) (#9662) and anti-poly [ADP-ribose] polymerase (PARP) (#9542) were from Cell Signaling Technology. The primary antibodies anti-FHF1 antibody (#PA5-67182) was from Thermo Fisher Scientific. Anti-tubulin primary antibody (#T6557) were from Sigma-Aldrich. Anti-human IgG (Fc) antibody coupled to HRP (#ab97225) was from Abcam. Horseradish peroxidase-conjugated secondary antibodies were from Jackson ImmunoResearch and chemiluminescent substrate was used for their visualization in the ChemiDoc station (BioRad). heparin Sepharose resin was from GE Healthcare. Geneticin (G-418) was from BioShop. PD173074 and heparin were from Sigma-Aldrich.