Anti tcr vδ1

Manufactured by Miltenyi Biotec

Sourced in United States

The Anti-TCR Vδ1 is a lab reagent designed to detect the expression of the Vδ1 T cell receptor on cell samples. It provides a straightforward method to identify and quantify Vδ1-positive cells.

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Lab products found in correlation

Facscanto a, by BD (1 mentions) Ficoll hypaque, by GE Healthcare (1 mentions) Anti tcrγδ, by BD (1 mentions) Fixable near ir dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Lsrfortessa cell analyzer, by BD (1 mentions) Anti cd8, by BD (1 mentions) Anti cd4, by BD (1 mentions) Anti cd3, by BioLegend (1 mentions) Anti tcrγδ, by BioLegend (1 mentions)

2 protocols using anti tcr vδ1

Identification of T-cell Subsets from PBMCs

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Peripheral blood mononuclear cells (PBMCs) were isolated from peripheral blood by standard Ficoll-Hypaque (GE Healthcare) density gradient centrifugation. Dead cells were excluded using fixable viability dye Fixable Near-IR Dead Cell Stain Kit (Invitrogen; dilution 1:10). For surface marker staining, cells were incubated with antibodies at 4°C for 30 min and then washed and fixed. The following fluorescence antibodies were applied: anti-TCR Vγ9 (BioLegend; clone B3), anti-CR Vδ2 (BioLegend; clone B6), anti-TCRγδ (BD; clone B1), anti-TCR Vδ1, human (Miltenyi; clone REA 173), and anti-CD3 (Biolegend, clone UCHT1). All samples were acquired with a BD FACS CANTO A (BD Biosciences) and analyzed with FlowJo software.

Ruggieri M., Ducasa N., Juraske C., Polo V.G., Berini C., Quiroga M.F., Christopoulos P., Minguet S., Biglione M, & Schamel W.W. (2022). Phenotypic and functional analysis of γδ T cells in the pathogenesis of human T-cell lymphotropic virus type 1 infection. Frontiers in Immunology, 13, 920888.

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Immunophenotyping post-haploHCT

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At days 30, 60, and 90 after haploHCT, peripheral blood samples were collected from recipients and stained with the fluorochrome-labeled antibodies: including anti-CD3, anti-TCRγδ, and anti-TCRVδ2 purchased from BioLegend (USA); anti-TCRαβ, anti-CD4, and anti-CD8 bought from Becton Dickinson (BD, USA); anti-TCRVδ1 from Miltenyi Biotec (Germany). Polychromatic flow cytometric analyses were performed on a BD LSRFortessaTM Cell Analyzer and further analyzed using BD FACSDivaTM software.

Liu J., Gao H., Xu L.P., Mo X.D., Liu R., Liang S., Wu N., Wang M., Wang Z., Chang Y.J., Wang Y., Zhang X.H, & Huang X.J. (2020). Immunosuppressant indulges EBV reactivation and related lymphoproliferative disease by inhibiting Vδ2+ T cells activities after hematopoietic transplantation for blood malignancies. Journal for Immunotherapy of Cancer, 8(1), e000208.

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