Legs and details of the male and female genitalia were drawn by means of an Abbe’s drawing apparatus on a compound microscope (Zeiss Jenaval) at magnification 130–500×. Wings were photographed on an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult (dry-mounted) specimens and heads were photographed by means of a Canon EOS 5D Mark III digital camera with a Nikon CFI Plan 10×/0.25NA 10.5mm WD objective attached to a Canon EF 70–200mm f/4L USM zoom lens. The specimen photographed by means of the latter equipment was repositioned upwards between each exposure using a Cognisys StackShot Macro Rail and the final photograph was compiled from multiple layers (~ 40) using Helicon Focus Pro 7.0.2. The final images were edited in Adobe Photoshop CS6. All morphological illustrations were prepared by the first author.
Cfi plan 10 0.25na 10 5mm wd objective
Manufactured by Canon
The CFI Plan 10x/0.25NA 10.5mm WD objective is a microscope objective lens manufactured by Canon. It has a magnification of 10x and a numerical aperture (NA) of 0.25. The working distance (WD) of this objective is 10.5 millimeters.
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Lab products found in correlation
3 protocols using cfi plan 10 0.25na 10 5mm wd objective
1
Detailed Morphological Documentation of Insects
2
Detailed Microscopic Documentation of Insect Morphology
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Legs were drawn on squared paper using a Reichert binocular microscope with an ocular screen. Details of the male and female genitalia were drawn by means of Abbe’s drawing apparatus on a compound microscope (Zeiss Jenaval) at larger magnification (130–500×). Wings were photographed on the compound microscope Olympus BX51 with an attached digital camera (Canon EOS 1200D). Whole adult (dry-mounted) specimens and wings were photographed by means of a digital camera Canon EOS 5D Mark III with a Nikon CFI Plan 10×/0.25NA 10.5 mm WD objective attached to a Canon EF 70–200 mm f/4L USM zoom lens. The specimen photographed by means of the latter equipment was repositioned upwards between each exposure using a Cognisys StackShot Macro Rail and the final photograph was compiled from multiple layers (20–40) using Helicon Focus Pro 7.0.2. The final images were edited in Adobe Photoshop CS6.
3
Detailed Morphological Documentation Protocol
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Legs were drawn on squared paper using a Reichert binocular microscope with an ocular screen. Details of the male and female genitalia and all preimaginal stages were drawn by means of an Abbe's drawing apparatus on a compound microscope (JENAVAL) at larger magnification (130-500 ×). Wings were photographed using an Olympus BX51 compound microscope with an attached digital camera (Canon EOS 1200D). Whole adult specimens and their details were photographed by P. Krásenský by means of a Canon 5D Mark II digital camera with a Nikon CFI Plan 10 × / 0.25NA 10.5 mm WD objective attached on a Canon EF 70-200 mm f / 4L USM lens. The specimens were moved upwards between each exposure using a WeMacro Rail (http://www.wemacro.com/, each step was 0.01 mm) and the final photographs were stacked from multiple layers using Zerene Stacker. The final images were edited in Adobe Photoshop CS6.
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