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Horseradish peroxidase hrp linked rabbit igg secondary antibody

Manufactured by Cell Signaling Technology
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Horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody is a laboratory reagent used to detect and quantify target proteins in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry. The antibody is produced by immunizing rabbits with IgG and conjugating the resulting antibodies with horseradish peroxidase, an enzyme that catalyzes a colorimetric reaction for signal detection.

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Lab products found in correlation

Phospho akt, by Cell Signaling Technology (2 mentions) Stat3, by Cell Signaling Technology (2 mentions) Phospho stat3, by Cell Signaling Technology (2 mentions) Epidermal growth factor (egf), by R&D Systems (1 mentions) Phospho pi3k, by Cell Signaling Technology (1 mentions) Phospho stat5, by Cell Signaling Technology (1 mentions) Phospho plcγ1, by Cell Signaling Technology (1 mentions) Anti phospho egfr, by Cell Signaling Technology (1 mentions) Plcγ1, by Cell Signaling Technology (1 mentions) Phospho src, by Cell Signaling Technology (1 mentions) Anti phospho egfr, by Merck Group (1 mentions) Pfrs2 y196, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Tgf β, by Cell Signaling Technology (1 mentions) Anti fgfr1, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) Heparin sulfate, by STEMCELL (1 mentions)

Close spelling variants of this product

Horseradish peroxidase (175 citations) HRP-linked secondary antibody (124 citations) Horseradish peroxidase-linked secondary antibodies (63 citations) HRP-linked antibody (54 citations) Horseradish peroxidase (HRP)-linked secondary antibodies (40 citations) Horseradish peroxidase (HRP) (40 citations) Horseradish peroxidase (HRP)-linked antibody (9 citations) IgG rabbit (9 citations) Anti-rabbit IgG horseradish peroxidase (HRP)-linked secondary antibody (8 citations) IgG horseradish peroxidase–linked secondary antibody (4 citations)

2 protocols using horseradish peroxidase hrp linked rabbit igg secondary antibody

1

EGF-Mediated Signaling Pathway Analysis

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Epidermal growth factor (EGF) was purchased from R&D Systems (Minneapolis, MN). We utilized the following antibodies: anti-phospho-EGFR (to phosphotyrosines 1045, 1068, 1148, and 1173), total EGFR, Akt (#4691), phospho-Akt (Thr308, #9275), MAPK (#4695), phospho-MAPK (Thr202/Tyr204, #4370), PI3K (#11889), phospho-PI3K (Tyr458, #4228), PLCγ1 (#5690), phospho-PLCγ1 (Tyr783, #2821), Src (#2123), phospho-Src (Tyr416, #2101), Stat3 (#4904), phospho-Stat3 (Tyr705, #9145), phospho-Stat5 (Tyr694, #4322), Cbl (#2747),and Horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody from Cell Signaling (Danvers, MA);anti-phospho-EGFR (phosphotyrosines845 and 992) from Millipore (Billerica, MA); and Stat5 (sc-835), phospho-Cbl (Tyr700, sc-377571), and goat anti-mouse IgM-HRP (sc-2064) from Santa Cruz Biotechnology (Dallas, TX).
Kim Y., Apetri M., Luo B., Settleman J.E, & Anderson K.S. (2015). Differential Effects of Tyrosine Kinase Inhibitors on Normal and Oncogenic EGFR Signaling and Downstream Effectors. Molecular cancer research : MCR, 13(4), 765-774.
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2

FGF2 and FGFR1 Signaling Pathway Assay

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Recombinant FGF2 was purchased from Gibco (#PHG0024). Heparin sulfate was purchased from StemCell Technologies (#07980). The following antibodies were used (all primary antibodies are rabbit derived unless otherwise noted): anti-FGFR1 (#9740), phospho-FGFR (Y653/654, #3476, mouse derived), phospho-FRS2 (Y436, #3861; Y196, #3864), PLCγ (#5690), phospho-PLCγ (Y783, #2821), ERK (#4695), phospho-ERK (T202/Y204 #4370), Akt (#4691), phospho-Akt (S473 #4060), STAT3 (#4904), phospho-STAT3 (Y705, #9145), E-cadherin (#3195), Vimentin (#5741), ZEB1 (#3396), N-cadherin (#13116), HIF1α (#14179), Bcl-XL (#2764), Hsp90 (#4877), FGF2 (#61977), TGFβ (#3711), Cyclin D1 (#2978), GAPDH (#2118), horseradish peroxidase (HRP)-linked rabbit IgG secondary antibody (#7074) and HRP-linked mouse IgG secondary antibody (#7076), from Cell Signaling Technology. Anti-FRS2 (#10425, mouse derived) was purchased from Abcam. AZD4547 was purchased from Selleck Chemistry. The following CyTOF antibodies were purchased from Fluidigm Inc.: STAT3 (#3173003A), pSTAT3 (#3158005A), Vimentin (#3154014A), TGFβ (#3163010B), SOX2 (#3150019B), and Nanog (#3169014A). Custom antibody-metal conjugations were performed using kits purchased from Fluidigm, Inc. (PRD002) and carrier-free antibodies targeting FGFR1 (#9740), pFGFR1 (#3476), pFRS2 Y196 (#3864), and ZEB1 (#3396) were purchased from Cell Signaling Technology.
Ryan M.R., Sohl C.D., Luo B, & Anderson K.S. (2018). The FGFR1 V561M Gatekeeper Mutation Drives AZD4547 Resistance through STAT3 Activation and EMT. Molecular cancer research : MCR, 17(2), 532-543.
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