Rabbit anti tom20

Manufactured by Proteintech

Sourced in United States

Rabbit anti-TOM20 is a primary antibody that recognizes the translocase of outer mitochondrial membrane 20 (TOM20) protein. TOM20 is a key component of the protein import machinery in the outer mitochondrial membrane and plays a crucial role in the transport of proteins into the mitochondria. This antibody can be used to detect and study the expression and localization of TOM20 in various cell and tissue types.

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Lab products found in correlation

Prolong diamond, by Thermo Fisher Scientific (2 mentions) Mantra, by PerkinElmer (1 mentions) Goat anti rabbit igg, by Santa Cruz Biotechnology (1 mentions) Rabbit anti α sma, by Proteintech (1 mentions) Rabbit anti cx43, by Cell Signaling Technology (1 mentions) Alexa fluor 594 goat anti mouse igg h l, by Proteintech (1 mentions) Hoechst 33342, by Beyotime (1 mentions) Rabbit anti tom20, by Cell Signaling Technology (1 mentions) Mouse anti tom70, by Santa Cruz Biotechnology (1 mentions) Rabbit anti bip, by Cell Signaling Technology (1 mentions) Mouse anti strep mab, by IBA Lifesciences (1 mentions) Rabbit anti tom40, by Santa Cruz Biotechnology (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Mouse anti β tubulin, by Merck Group (1 mentions) Mouse anti strep tag, by Qiagen (1 mentions) Mouse anti eea1, by BD (1 mentions) Rabbit anti mmp9, by Proteintech (1 mentions) Electron microscopy grade paraformaldehyde, by Electron Microscopy Sciences (1 mentions) Triton x 100, by Merck Group (1 mentions) Mouse anti dlp1, by BD (1 mentions)

Close spelling variants of this product

TOM20 (26 citations) Anti-Tom20 (18 citations) Anti-TM (2 citations)

6 protocols using rabbit anti tom20

Immunohistochemical Staining of Cell Markers

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Tissue sections of 3 µm thickness were baked in oven at 62°C for 6 h before dewaxing and rehydration. The slides were washed with phosphate buffered saline (PBS) three times before and after heat-induced antigen retrieval. LX-2 cells seeded on coverslips were fixed in methanol for 20 m and permeabilized with 0.1% Triton X-100 (Genview Life Scientific Inc, Tampa, FL, USA) for more than 10 m. Endogenous peroxidases were blocked with 3% H₂O₂ for 20 m. Nonspecific staining was preblocked with PBS containing 2.5% bovine serum albumin. The primary antibodies were rabbit anti-Cx43 (Cell Signaling Technology; Danvers, Massachusetts, USA, #3512S, 1:500), rabbit anti-TOM20 (Proteintech; #11802-1-AP, 1:1,000), rabbit anti-α-SMA (Proteintech; #14395-1-AP, 1:200) or chicken anti-glial fibrillary acidic protein (GFAP) (Abcam, Cambridge, UK; #ab4674) at 4°C overnight. The secondary antibodies were goat anti-rabbit IgG (Santa Cruz Biotechnology, Dallas, TX, USA; 1:200), Alexa Fluor 594 goat anti-mouse IgG (H+L); Proteintech; #SA00006-3) or Goat anti-chicken IgY H+L (Alexa Fluor 647; Abcam; ab150171) or Hoechst 33342 (Beyotime Biotechnology, Shanghai, China) for 10 m. Stained sections were scanned by fluorescence optical microscopy (Mantra; PerkinElmer).

Cao Y., Xia Y., Wang Y., Shi H., Wu Y, & Lu Y. (2022). MgIG Attenuates Oxaliplatin-induced Hepatotoxicity through Suppression of Connexin 43 in Hepatic Stellate Cells. Journal of Clinical and Translational Hepatology, 11(3), 584-594.

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Immunofluorescence Staining of SARS-CoV-2 Organelles

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Rabbit anti-beta-actin (Cell Signaling Technology #4967, RRID:AB_330288); mouse anti-beta tubulin (Sigma-Aldrich #T8328, RRID:AB_1844090); rabbit anti-BiP (Cell Signaling Technology #3177S, RRID:AB_2119845); mouse anti-EEA1 (BD Biosciences #610457, RRID:AB_397830, used at 1:200); mouse anti-ERGIC53 (Enzo Life Sciences #ALX-804-602-C100, RRID:AB_2051363, used at 1:200); anti-GM130; rabbit anti-GRP78 BiP (Abcam #Ab21685, RRID:AB_2119834); rabbit anti–SARS-CoV–nucleocapsid protein (NP) (Rockland #200-401-A50, RRID:AB_828403); rabbit anti-PDI (protein disulfide isomerase) (Cell Signaling Technology #3501, RRID:AB_2156433); mouse anti-Strep tag (QIAGEN #34850, RRID:AB_2810987, used at 1:5000); mouse anti-strepMAB (IBA Lifesciences #2-1507-001, used at 1:1000); rabbit anti–Strep-tag II (Abcam #ab232586); rabbit anti-Tom20 (Proteintech #11802-1-AP, RRID:AB_2207530, used at 1:1000); rabbit anti-Tom20 (Cell Signaling Technology #42406, RRID:AB_2687663); mouse anti-Tom22 (Santa Cruz Biotechnology #sc-101286, RRID:AB_1130526); rabbit anti-Tom40 (Santa Cruz Biotechnology #sc-11414, RRID:AB_793274); mouse anti-Tom70 (Santa Cruz #sc-390545, RRID:AB_2714192, used at 1:500); Rabbit anti-STX5 (Synaptic Systems 110 053, used at 1:500); and ActinStaining Kit 647-Phalloidin (Hypernol #8817-01, used at 1:400).

Gordon D.E., Hiatt J., Bouhaddou M., Rezelj V.V., Ulferts S., Braberg H., Jureka A.S., Obernier K., Guo J.Z., Batra J., Kaake R.M., Weckstein A.R., Owens T.W., Gupta M., Pourmal S., Titus E.W., Cakir M., Soucheray M., McGregor M., Cakir Z., Jang G., O’Meara M.J., Tummino T.A., Zhang Z., Foussard H., Rojc A., Zhou Y., Kuchenov D., Hüttenhain R., Xu J., Eckhardt M., Swaney D.L., Fabius J.M., Ummadi M., Tutuncuoglu B., Rathore U., Modak M., Haas P., Haas K.M., Naing Z.Z., Pulido E.H., Shi Y., Barrio-Hernandez I., Memon D., Petsalaki E., Dunham A., Marrero M.C., Burke D., Koh C., Vallet T., Silvas J.A., Azumaya C.M., Billesbølle C., Brilot A.F., Campbell M.G., Diallo A., Dickinson M.S., Diwanji D., Herrera N., Hoppe N., Kratochvil H.T., Liu Y., Merz G.E., Moritz M., Nguyen H.C., Nowotny C., Puchades C., Rizo A.N., Schulze-Gahmen U., Smith A.M., Sun M., Young I.D., Zhao J., Asarnow D., Biel J., Bowen A., Braxton J.R., Chen J., Chio C.M., Chio U.S., Deshpande I., Doan L., Faust B., Flores S., Jin M., Kim K., Lam V.L., Li F., Li J., Li Y.L., Li Y., Liu X., Lo M., Lopez K.E., Melo A.A., Moss FR I.I.I., Nguyen P., Paulino J., Pawar K.I., Peters J.K., Pospiech TH J.r., Safari M., Sangwan S., Schaefer K., Thomas P.V., Thwin A.C., Trenker R., Tse E., Tsui T.K., Wang F., Whitis N., Yu Z., Zhang K., Zhang Y., Zhou F., Saltzberg D., Hodder A.J., Shun-Shion A.S., Williams D.M., White K.M., Rosales R., Kehrer T., Miorin L., Moreno E., Patel A.H., Rihn S., Khalid M.M., Vallejo-Gracia A., Fozouni P., Simoneau C.R., Roth T.L., Wu D., Karim M.A., Ghoussaini M., Dunham I., Berardi F., Weigang S., Chazal M., Park J., Logue J., McGrath M., Weston S., Haupt R., Hastie C.J., Elliott M., Brown F., Burness K.A., Reid E., Dorward M., Johnson C., Wilkinson S.G., Geyer A., Giesel D.M., Baillie C., Raggett S., Leech H., Toth R., Goodman N., Keough K.C., Lind A.L., Klesh R.J., Hemphill K.R., Carlson-Stevermer J., Oki J., Holden K., Maures T., Pollard K.S., Sali A., Agard D.A., Cheng Y., Fraser J.S., Frost A., Jura N., Kortemme T., Manglik A., Southworth D.R., Stroud R.M., Alessi D.R., Davies P., Frieman M.B., Ideker T., Abate C., Jouvenet N., Kochs G., Shoichet B., Ott M., Palmarini M., Shokat K.M., García-Sastre A., Rassen J.A., Grosse R., Rosenberg O.S., Verba K.A., Basler C.F., Vignuzzi M., Peden A.A., Beltrao P, & Krogan N.J. (2020). Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms. Science (New York, N.y.), 370(6521), eabe9403.

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Antibody-based protein detection protocol

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The following antibodies were used: rabbit anti-MMP9 (Proteintech, cat.no.: 10375-2-AP); rabbit anti-Ki67 (Thermo Fisher Scientific Invitrogen, MA5-14520); rabbit anti-Gsta1 (Proteintech, cat.no.: 14475-1-AP); rabbit anti-Col3a1 (Proteintech, cat.no.: 22734-1-AP); rabbit anti-Tom20 (Proteintech, cat.no.: 11802-1-AP) and rabbit anti-SGLT2 (gift. Of H. Koepsell).

Dahlke E., Engmann T., Anan Y., Häsler R., Solinas G, & Theilig F. (2022). Tubular IKKβ Deletion Alleviates Acute Ischemic Kidney Injury and Facilitates Tissue Regeneration. International Journal of Molecular Sciences, 23(17), 10199.

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Immunofluorescence Staining of Mitochondrial Proteins

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Cells were seeded onto coverslips and cultured for 2 hr prior to fixing and staining. Thereafter cells were fixed with 4% electron microscopy grade paraformaldehyde (Electron Microscopy Sciences) for 10 min at RT and then permeabilized for 3 minutes with 0.2% Triton-X 100 (Millipore Sigma). Over-extracted cells were treated with 0.4% Triton-X 100 for 10 min. Cells were then washed three times with DPBS and stained overnight at 4°C with primary antibodies diluted in PBS. Next, they were washed twice with PBS for 5 min, incubated with secondary antibodies (diluted 1:1000) for 2 hr at RT in PBS. Actin filaments were stained with Alexa Fluor 488, 568 or 647 phalloidin (Life Technologies) for 30 min at RT in DPBS. Cells were washed three times with DPBS before mounting with Prolong Diamond (Life Technologies). The following antibodies were used: Rabbit anti-Tom 20 (cat# 11802-1-AP, Proteintech) 1:300 dilution; rabbit-anti-parkin (cat # PA5-13399, Invitrogen) 1:50 dilution, mouse anti-DLP1 (cat# 611113 Clone 8/DLP1 (RUO), BD biosciences) 1:100 dilution, Preabsorbed secondary antibodies used were anti-mouse IgG 647, anti-rabbit IgG 568 at a 1:1000 dilution. Coverslips were mounted onto slides using Prolong Diamond (Thermo Fisher). All slides were cured at RT in the dark for 2 days prior to imaging.

Read T.A., Cisterna B.A., Skruber K., Ahmadieh S., Lindamood H.L., Vitriol J.A., Shi Y., Lefebvre A.E., Black J.B., Butler M.T., Bear J.E., Cherezova A., Ilatovskaya D.V., Weintraub N.L, & Vitriol E.A. (2023). The actin binding protein profilin 1 is critical for mitochondria function. bioRxiv.

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Immunofluorescent Staining of Cultured Cells

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Cells were fixed with 4% paraformaldehyde (ThermoFisher, 50980487) for 15 min at 37 °C. Coverslips were incubated in blocking solution (5% goat serum (Sigma, G9023) and 0.3% Triton-X100 (Acros Organics, 215682500) in PBS) for 60 min. Coverslips were then incubated in primary antibody solution (1:1000 Mouse Anti-ATPB (Abcam, ab14730), 1:200 Rabbit Anti-TOM20 (ProteinTech, 11802–1-AP), 1:1000 Chicken Anti-MAP2 (Abcam, ab5392), 1% BSA (Sigma, A9647) and 0.3% Triton-X100 in PBS) at 4 °C overnight. After primary incubation, coverslips were washed 3 × in PBS and incubated in secondary antibody solution (1:200 Anti-Mouse Alexa Fluor 488 (Invitrogen, A11029), 1:200 Anti-Rabbit Alexa Fluor 546 (Invitrogen, A10040), 1:200 Anti-Chicken Alexa Fluor 647 (Invitrogen, A21449), 1% BSA (Sigma, A9647) and 0.3% Triton-X100 in PBS) for 60 min. Following secondary incubation, coverslips were washed 3 × with PBS and mounted on glass slides using Fluoroshield with DAPI (Sigma, F6057).

Fogo G.M., Anzell A.R., Maheras K.J., Raghunayakula S., Wider J.M., Emaus K.J., Bryson T.D., Bukowski M.J., Neumar R.W., Przyklenk K, & Sanderson T.H. (2021). Machine learning-based classification of mitochondrial morphology in primary neurons and brain. Scientific Reports, 11, 5133.

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Comprehensive Antibody Panel for OXPHOS

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The following antibodies were used in our work: rabbit anti-NDUFB8 (Proteintech, 1479-1-AP); mouse anti-MTCO1 (Abcam, ab14705); mouse anti-total OXPHOS antibody cocktail (Abcam, ab110413); mouse anti-GFP (Roche, 11814460001); rabbit anti-VPS39 (Proteintech, 16219-1-AP and Novus Biologicals, NBP1-76535); mouse anti-KDEL (Millipore, 10C3); mouse anti-TOM40 (Santa Cruz, sc-365467); rabbit anti-TOM20 (Proteintech, 11802-1-AP); rabbit anti-LC3B (Sigma, L7543); rabbit anti-MICU1 (Sigma, PA5-83371); guinea pig anti-p62 (Progen, GP62-C); rabbit anti-pS473-AKT (Cell Signaling, 4060S), rabbit anti-AKT (Cell Signaling, 4685); rabbit anti-pT246-PRAS40 (Cell Signaling, 13175); anti-PRAS40 (Cell Signaling, 2691); rabbit anti-GAPDH (Santa Cruz, sc-25778); mouse anti-actin (Sigma, A5316 and abcam, ab14128); mouse anti-tubulin (Sigma, T6074); rabbit anti-SGPL1 (Atlas Antibodies, HPA021125); rabbit anti-AIF (Cell Signaling, 5318).

Jackson J., Wischhof L., Scifo E., Pellizzer A., Wang Y., Piazzesi A., Gentile D., Siddig S., Stork M., Hopkins C.E., Händler K., Weis J., Roos A., Schultze J.L., Nicotera P., Ehninger D, & Bano D. (2022). SGPL1 stimulates VPS39 recruitment to the mitochondria in MICU1 deficient cells. Molecular Metabolism, 61, 101503.

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