The content of H2O2 of cotton root was determined using a Micro Hydrogen Peroxide Assay Kit (BC3590, Solarbio, Beijing, China) and AsA was measured using an Ascorbic Acid Assay Kit (BC1230, Solarbio, Beijing, China) based on the methods in Wu et al. (2017) (link).
Ascorbic acid assay kit
Manufactured by Solarbio
Sourced in China
The Ascorbic Acid Assay Kit is a laboratory equipment used to measure the concentration of ascorbic acid (vitamin C) in various samples. The kit provides a colorimetric detection method that allows for quantitative analysis of ascorbic acid levels.
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Lab products found in correlation
2 protocols using ascorbic acid assay kit
1
Quantifying H2O2 and AsA in Cotton Roots
2
Uptake of AA-Loaded mExo by HCECs
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After the completion of the reduction reaction, the residual NaBH 4 and AA were removed by centrifugation at 100 000g for 30 min. The concentrations of AA in the supernatant and solution before the reaction were measured using the Ascorbic Acid Assay Kit (Beijing Solarbio Science & Technology Co., Ltd). The loading capacity of AA was calculated using the following equation.
where c b and c a are the concentrations of AA before and after the reaction and c mExo is the concentration of mExo. nM mg -1 is the amount of AA on the surface of 1 mg of mExo.
3.6. Uptake of mExo@AA by human cultured corneal endothelial cells (HCECs)
The mExo@AA solution was stained with DiD for 1 h at 37 °C. The excess dye was removed using exosome spin columns (Thermo Fisher). HCECs were incubated with mExo@AA for 12 h, followed by fixation and imaging.
where c b and c a are the concentrations of AA before and after the reaction and c mExo is the concentration of mExo. nM mg -1 is the amount of AA on the surface of 1 mg of mExo.
3.6. Uptake of mExo@AA by human cultured corneal endothelial cells (HCECs)
The mExo@AA solution was stained with DiD for 1 h at 37 °C. The excess dye was removed using exosome spin columns (Thermo Fisher). HCECs were incubated with mExo@AA for 12 h, followed by fixation and imaging.
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