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Vs110 slide scanner microscope

Manufactured by Olympus
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The VS110 slide scanner microscope is a high-performance digital imaging system designed for scanning and digitizing microscope slides. The device captures high-resolution images of biological samples and tissue sections, enabling digital archiving and analysis of microscopic specimens.

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Lab products found in correlation

Biocytin, by Thermo Fisher Scientific (2 mentions) Picrosirius red staining kit, by Abcam (1 mentions)

4 protocols using vs110 slide scanner microscope

1

Subthalamic Nucleus Labeling in Mice

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Acute parasagittal slices (350 μm thick, 10° off-sagittal) were prepared from wild type mice (post natal day 34) as described above. This cutting orientation preserves the reciprocal connections between the subthalamic nucleus (STN) and the GP42 (link). In circulating warm ACSF (32–34°C), patch pipets filled with biocytin (0.2%, Molecular Probes) containing internal solution containing (in mM) 135 mM KMeSO4, 5 mM KCl, 5 mM HEPES, 4 mM Mg-ATP, 0.3 mM Na2-Phosphocreatine (pH 7.4 adjusted using KOH; 295 mOsm·kg−1) were targeted to the STN under DIC illumination. A picospritzer (Picospritzer III; Parker Instrumentation) was used to puff the solution into the center of STN for 1 hour (400 ms pulse at 1 Hz, 5–10 PSI). The slice was allowed to recover for 0.5 hour before being transferred to 4% PFA in 1x PBS overnight. The following morning, slices were rinsed in 1x PBS before avidin-biotinylated HRP complex (ABC) processing (Vectastain)43 (link). Slices were then wet-mounted onto glass slides, coverslipped, and imaged under bright-field illumination using the 10x objective of an Olympus VS110 slide scanner microscope.
Saunders A., Oldenburg I.A., Berezovskii V.K., Johnson C.A., Kingery N.D., Elliott H.L., Xie T., Gerfen C.R, & Sabatini B.L. (2015). A direct GABAergic output from the basal ganglia to frontal cortex. Nature, 521(7550), 85-89.
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2

Subthalamic Nucleus Labeling in Mice

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Acute parasagittal slices (350 μm thick, 10° off-sagittal) were prepared from wild type mice (post natal day 34) as described above. This cutting orientation preserves the reciprocal connections between the subthalamic nucleus (STN) and the GP42 (link). In circulating warm ACSF (32–34°C), patch pipets filled with biocytin (0.2%, Molecular Probes) containing internal solution containing (in mM) 135 mM KMeSO4, 5 mM KCl, 5 mM HEPES, 4 mM Mg-ATP, 0.3 mM Na2-Phosphocreatine (pH 7.4 adjusted using KOH; 295 mOsm·kg−1) were targeted to the STN under DIC illumination. A picospritzer (Picospritzer III; Parker Instrumentation) was used to puff the solution into the center of STN for 1 hour (400 ms pulse at 1 Hz, 5–10 PSI). The slice was allowed to recover for 0.5 hour before being transferred to 4% PFA in 1x PBS overnight. The following morning, slices were rinsed in 1x PBS before avidin-biotinylated HRP complex (ABC) processing (Vectastain)43 (link). Slices were then wet-mounted onto glass slides, coverslipped, and imaged under bright-field illumination using the 10x objective of an Olympus VS110 slide scanner microscope.
Saunders A., Oldenburg I.A., Berezovskii V.K., Johnson C.A., Kingery N.D., Elliott H.L., Xie T., Gerfen C.R, & Sabatini B.L. (2015). A direct GABAergic output from the basal ganglia to frontal cortex. Nature, 521(7550), 85-89.
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3

Histological Evaluation of Glomerulosclerosis

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Silver staining was done according to Jones' Methenamine Silver Stain (JMS) -Staining protocol (29) . Stained slides were scanned using an Olympus VS110 slide scanner microscope. Five randomly chosen high-power fields in the renal cortical section (magnification 200X) were taken, and glomerulosclerosis scores were evaluated by two independent investigators blinded to experimental conditions. Based on involved glomerular percentage, glomerulosclerosis score was classified as 0-3 (0: no glomerulopathy-double contours affecting <10% peripheral capillary loop in the most severe attacked glomerulus. 1: double contours affecting up to 25% peripheral capillary loop in most affected non-sclerotic glomeruli. 2: double contours affecting up to 50% peripheral capillary loop in most affected non-sclerotic glomeruli. 3: double contours affecting >50% peripheral capillary loop in most affected non-sclerotic glomeruli) (30) .
Lan S., Yang B., Migneault F., Turgeon J., Bourgault M., Dieudé M., Cardinal H., Hickey M.J., Patey N, & Hébert M.J. (2021). Caspase-3-dependent peritubular capillary dysfunction is pivotal for the transition from acute to chronic kidney disease after acute ischemia-reperfusion injury. American journal of physiology. Renal physiology, 321(3).
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4

Quantifying Interstitial Fibrosis via Sirius Red

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Sirius Red staining was performed using Picro-Sirius Red Staining Kit (ab150681, Abcam, Cambridge, MA, USA) according to the manufacturer's instructions. All slides were scanned using an Olympus VS110 slide scanner microscope. Five randomly chosen high-power fields at the cortical-medullary junction (magnification 200X) were taken. Sirius red positive area within PTC and glomeruli were evaluated with ImageJ (National Institutes of Health) by two independent investigators blinded to experimental conditions.
Lan S., Yang B., Migneault F., Turgeon J., Bourgault M., Dieudé M., Cardinal H., Hickey M.J., Patey N, & Hébert M.J. (2021). Caspase-3-dependent peritubular capillary dysfunction is pivotal for the transition from acute to chronic kidney disease after acute ischemia-reperfusion injury. American journal of physiology. Renal physiology, 321(3).
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