Cd4 apc clone rm4 5

Manufactured by BioLegend

CD4-APC (clone RM4-5) is a fluorochrome-conjugated monoclonal antibody that binds to the CD4 antigen, which is expressed on the surface of T helper cells. It is used in flow cytometry applications to identify and quantify CD4+ T cells in biological samples.

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Lab products found in correlation

Cd45 bv605 clone 30 f11, by BD (2 mentions) Facsymphony a5 cytometer, by FlowJo (2 mentions) Cd11b pe clone m1 70, by BioLegend (2 mentions) Cd11c fitc clone n418, by Cytek Biosciences (2 mentions) Cd3e pe cd594 clone 145 2c11, by BD (2 mentions) Cd62l pe cy7 clone mel 14, by Cytek Biosciences (2 mentions) Cd8a a700 clone 53 6, by BioLegend (2 mentions) Ly6g bv421 clone 1a8, by BD (2 mentions) B220 buv496 clone ra3 6b2, by BD (2 mentions) Strataclean resin, by Agilent Technologies (1 mentions) Atp tlrl atpl, by InvivoGen (1 mentions) Anti ha h6908, by Merck Group (1 mentions) Aicar s1802, by Selleck Chemicals (1 mentions) Compound c p5499, by Merck Group (1 mentions) Pt172 ampk, by Cell Signaling Technology (1 mentions) Anti gsdmd ab219800, by Abcam (1 mentions) Anti il18 a1115, by ABclonal (1 mentions) Cd8 percp cy5.5 clone 53 6 7, by BioLegend (1 mentions) Cd19 percp cy5, by Cytek Biosciences (1 mentions)

3 protocols using cd4 apc clone rm4 5

AMPK Signaling Pathway Modulation

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LPS O111:B4 (L2630), Doxycycline (D3447), 2-Deoxy-D-glucose (D8375) and compound C (P5499) were obtained from Sigma-Aldrich. Subcellular protein fractionation kit (78840) was obtained from Thermo Fisher. Metformin (S1950) and AICAR (S1802) were obtained from Selleck Chemicals. ATP (tlrl-atpl) was obtained from InvivoGen. StrataClean resin (400714) and chemical competent cells (200315) were obtained from Agilent.
For immunoblot, AMPKα1(#2795), AMPKα2 (#2757), AMPKα1/2 (#5831) and pT172-AMPK (#2535) were purchased from Cell Signaling Technology. Anti-Flag (F1804), anti-Flag (F7425) and anti-HA (H6908) were obtained from Sigma-Aldrich. Anti-GSDMD (ab219800) and anti-Caspase-1 (ab108362) were obtained from Abcam. Anti-β-Actin (sc-47778), anti-GST (sc-138) and anti-Na/K-ATPase α1 (sc-21712) were purchased from Santa Cruz Biotechnology. Anti-IL18 (A1115) was obtained from Abclonal Technology. phospho-GSDMD S46 was generated by Abclonal Technology.
For FCAS analysis, CD45-APC-Cy7(clone 30-F11), CD3-FITC (clone 145-2C11), CD8-PerCP-Cy5.5 (clone 53-6.7) and CD4-APC (clone RM4-5) were obtained from BioLegend.

Chu X., Xiao X., Wang G., Uosef A., Lou X., Arnold P., Wang Y., Kong G., Wen M., Minze L.J, & Li X.C. (2023). Gasdermin D-mediated pyroptosis is regulated by AMPK-mediated phosphorylation in tumor cells. Cell Death & Disease, 14(7), 469.

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Comprehensive Murine Immune Cell Profiling

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Blood samples collected from cardiac punctures were first processed by lysing red blood cells, followed by staining with the following antibodies: CD11c FITC (clone N418, TONBO #350114U100, 1:600), CD19 PerCP‐Cy5.5 (clone 1D3, TONBO #650193U100, 1:480), CD11b PE (clone M1/70, Biolegend #101207, 1:960), CD3e PE‐CD594 (clone 145‐2C11, BD Biosciences #562286, 1:120), CD62L PE‐Cy7 (clone MEL‐14, TONBO #600621U100, 1:600), CD4 APC (clone RM4‐5, Biolegend #100516, 1:480), CD8a A700 (clone 53‐6.7, Biolegend #100729, 1:600), Ly6G BV421 (clone 1A8, BD Biosciences #562737, 1:480), CD45 BV605 (clone 30‐F11, BD Biosciences #563053, 1:240), B220 BUV496 (clone RA3‐6B2, BD Biosciences #564662, 1:120). Samples were processed on a FACSymphony A5 cytometer and analyzed using FlowJo (v10) software.

Heuer S.E., Keezer K.J., Hewes A.A., Onos K.D., Graham K.C., Howell G.R, & Bloss E.B. (2023). Control of hippocampal synaptic plasticity by microglia–dendrite interactions depends on genetic context in mouse models of Alzheimer's disease. Alzheimer's & Dementia, 20(1), 601-614.

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Multicolor Flow Cytometry Analysis

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Blood collected from cardiac punctures were first processed by lysing red blood cells, followed by staining with the following antibodies: CD11c FITC (clone N418, TONBO, 1:600), CD19 PerCP-Cy5.5 (clone 1D3, TONBO, 1:480), CD11b PE (clone M1/70, Biolegend, 1:960), CD3e PE-CD594 (clone 145–2C11, BD Biosciences, 1:120), CD62L PE-Cy7 (clone MEL-14, TONBO, 1:600), CD4 APC (clone RM4–5, Biolegend, 1:480), CD8a A700 (clone 53–6.7, Biolegend, 1:600), Ly6G BV421 (clone 1A8, BD Biosciences, 1:480), CD45 BV605 (clone 30-F11, BD Biosciences, 1:240), B220 BUV496 (clone RA3–6B2, BD Biosciences, 1:120). Samples were processed on a FACSymphony A5 cytometer and analyzed using FlowJo (v10) software.

Heuer S.E., Keezer K.J., Hewes A.A., Onos K.D., Graham K.C., Howell G.R, & Bloss E.B. (2023). Genetic context controls early microglia-synaptic interactions in mouse models of Alzheimer’s disease. bioRxiv.

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